The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear
Opioid peptides have been detected in the auditory and vestibular efferent neurons where they colocalize with the major neurotransmitter, acetylcholine. We investigated the function of opioids to modulate neurotransmission mediated by hair cell's α9/α10-containing nicotinic acetylcholine recept...
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todo:paper_10447431_v20_n4_p695_Lioudyno2023-10-03T15:58:18Z The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear Lioudyno, M.I. Verbitsky, M. Glowatzki, E. Holt, J.C. Boulter, J. Zadina, J.E. Elgoyhen, A.B. Guth, P.S. acetylcholine delta opiate receptor agonist dynorphin B endomorphin 1 enkephalin[2,5 dextro penicillamine] kappa opiate receptor agonist kappa opiate receptor antagonist mu opiate receptor agonist mu opiate receptor antagonist naloxone nicotinic receptor opiate peptide acetylcholine agents interacting with transmitter, hormone or drug receptors dynorphin dynorphin B endomorphin 1 endorphin enkephalin[2,5 dextro penicillamine] isoprotein nicotinic receptor oligopeptide opiate receptor animal cell animal tissue article competitive inhibition controlled study drug effect electric current electric potential evoked response female frog hair cell inner ear neurotransmission nonhuman oocyte priority journal protein expression protein interaction protein targeting rat reduction sacculus Xenopus animal chemistry cochlea cytology drug antagonism electric conductivity frogs and toads in vitro study inner ear metabolism physiology Sprague Dawley rat synapse vestibule Xenopus laevis Animalia Anura Acetylcholine Animals Anura Cochlea Dynorphins Ear, Inner Electric Conductivity Endorphins Enkephalin, D-Penicillamine (2,5)- Hair Cells Neurotransmitter Agents Oligopeptides Oocytes Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Nicotinic Receptors, Opioid Saccule and Utricle Synapses Xenopus laevis Opioid peptides have been detected in the auditory and vestibular efferent neurons where they colocalize with the major neurotransmitter, acetylcholine. We investigated the function of opioids to modulate neurotransmission mediated by hair cell's α9/α10-containing nicotinic acetylcholine receptors (α9/α10nAChRs). The endogenous opioid peptides, endomorphin-1 (mu agonist) and dynorphin B (kappa agonist), but not a delta agonist [D-Pen2,D-Pen-5]enkephalin, inhibited the acetylcholine-evoked currents in frog saccular hair cells and rat inner hair cells. This inhibition was noncompetitive, voltage-independent, and was accompanied by an acceleration of the rate of current decay. Selective mu- and kappa-opioid receptor antagonists did not block the inhibition, although partial reduction by naloxone was observed. All opioid antagonists tested also reduced the acetylcholine response. Endomorphin-1 and dynorphin B inhibited the acetylcholine-evoked currents in α9/α10-expressing Xenopus oocytes. Because oocytes lack opioid receptors, it provides strong evidence for the direct interaction of opioid peptides with α9/α10nAChR. Conclusion: α9/α10nAChR is a target for modulation by endomorphin-1 and dynorphin B, efferent cotransmitters in the inner ear. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10447431_v20_n4_p695_Lioudyno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetylcholine delta opiate receptor agonist dynorphin B endomorphin 1 enkephalin[2,5 dextro penicillamine] kappa opiate receptor agonist kappa opiate receptor antagonist mu opiate receptor agonist mu opiate receptor antagonist naloxone nicotinic receptor opiate peptide acetylcholine agents interacting with transmitter, hormone or drug receptors dynorphin dynorphin B endomorphin 1 endorphin enkephalin[2,5 dextro penicillamine] isoprotein nicotinic receptor oligopeptide opiate receptor animal cell animal tissue article competitive inhibition controlled study drug effect electric current electric potential evoked response female frog hair cell inner ear neurotransmission nonhuman oocyte priority journal protein expression protein interaction protein targeting rat reduction sacculus Xenopus animal chemistry cochlea cytology drug antagonism electric conductivity frogs and toads in vitro study inner ear metabolism physiology Sprague Dawley rat synapse vestibule Xenopus laevis Animalia Anura Acetylcholine Animals Anura Cochlea Dynorphins Ear, Inner Electric Conductivity Endorphins Enkephalin, D-Penicillamine (2,5)- Hair Cells Neurotransmitter Agents Oligopeptides Oocytes Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Nicotinic Receptors, Opioid Saccule and Utricle Synapses Xenopus laevis |
| spellingShingle |
acetylcholine delta opiate receptor agonist dynorphin B endomorphin 1 enkephalin[2,5 dextro penicillamine] kappa opiate receptor agonist kappa opiate receptor antagonist mu opiate receptor agonist mu opiate receptor antagonist naloxone nicotinic receptor opiate peptide acetylcholine agents interacting with transmitter, hormone or drug receptors dynorphin dynorphin B endomorphin 1 endorphin enkephalin[2,5 dextro penicillamine] isoprotein nicotinic receptor oligopeptide opiate receptor animal cell animal tissue article competitive inhibition controlled study drug effect electric current electric potential evoked response female frog hair cell inner ear neurotransmission nonhuman oocyte priority journal protein expression protein interaction protein targeting rat reduction sacculus Xenopus animal chemistry cochlea cytology drug antagonism electric conductivity frogs and toads in vitro study inner ear metabolism physiology Sprague Dawley rat synapse vestibule Xenopus laevis Animalia Anura Acetylcholine Animals Anura Cochlea Dynorphins Ear, Inner Electric Conductivity Endorphins Enkephalin, D-Penicillamine (2,5)- Hair Cells Neurotransmitter Agents Oligopeptides Oocytes Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Nicotinic Receptors, Opioid Saccule and Utricle Synapses Xenopus laevis Lioudyno, M.I. Verbitsky, M. Glowatzki, E. Holt, J.C. Boulter, J. Zadina, J.E. Elgoyhen, A.B. Guth, P.S. The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| topic_facet |
acetylcholine delta opiate receptor agonist dynorphin B endomorphin 1 enkephalin[2,5 dextro penicillamine] kappa opiate receptor agonist kappa opiate receptor antagonist mu opiate receptor agonist mu opiate receptor antagonist naloxone nicotinic receptor opiate peptide acetylcholine agents interacting with transmitter, hormone or drug receptors dynorphin dynorphin B endomorphin 1 endorphin enkephalin[2,5 dextro penicillamine] isoprotein nicotinic receptor oligopeptide opiate receptor animal cell animal tissue article competitive inhibition controlled study drug effect electric current electric potential evoked response female frog hair cell inner ear neurotransmission nonhuman oocyte priority journal protein expression protein interaction protein targeting rat reduction sacculus Xenopus animal chemistry cochlea cytology drug antagonism electric conductivity frogs and toads in vitro study inner ear metabolism physiology Sprague Dawley rat synapse vestibule Xenopus laevis Animalia Anura Acetylcholine Animals Anura Cochlea Dynorphins Ear, Inner Electric Conductivity Endorphins Enkephalin, D-Penicillamine (2,5)- Hair Cells Neurotransmitter Agents Oligopeptides Oocytes Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Nicotinic Receptors, Opioid Saccule and Utricle Synapses Xenopus laevis |
| description |
Opioid peptides have been detected in the auditory and vestibular efferent neurons where they colocalize with the major neurotransmitter, acetylcholine. We investigated the function of opioids to modulate neurotransmission mediated by hair cell's α9/α10-containing nicotinic acetylcholine receptors (α9/α10nAChRs). The endogenous opioid peptides, endomorphin-1 (mu agonist) and dynorphin B (kappa agonist), but not a delta agonist [D-Pen2,D-Pen-5]enkephalin, inhibited the acetylcholine-evoked currents in frog saccular hair cells and rat inner hair cells. This inhibition was noncompetitive, voltage-independent, and was accompanied by an acceleration of the rate of current decay. Selective mu- and kappa-opioid receptor antagonists did not block the inhibition, although partial reduction by naloxone was observed. All opioid antagonists tested also reduced the acetylcholine response. Endomorphin-1 and dynorphin B inhibited the acetylcholine-evoked currents in α9/α10-expressing Xenopus oocytes. Because oocytes lack opioid receptors, it provides strong evidence for the direct interaction of opioid peptides with α9/α10nAChR. Conclusion: α9/α10nAChR is a target for modulation by endomorphin-1 and dynorphin B, efferent cotransmitters in the inner ear. |
| format |
JOUR |
| author |
Lioudyno, M.I. Verbitsky, M. Glowatzki, E. Holt, J.C. Boulter, J. Zadina, J.E. Elgoyhen, A.B. Guth, P.S. |
| author_facet |
Lioudyno, M.I. Verbitsky, M. Glowatzki, E. Holt, J.C. Boulter, J. Zadina, J.E. Elgoyhen, A.B. Guth, P.S. |
| author_sort |
Lioudyno, M.I. |
| title |
The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| title_short |
The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| title_full |
The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| title_fullStr |
The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| title_full_unstemmed |
The α9/α10-containing nicotinic ACh receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin B, proposed efferent cotransmitters in the inner ear |
| title_sort |
α9/α10-containing nicotinic ach receptor is directly modulated by opioid peptides, endomorphin-1, and dynorphin b, proposed efferent cotransmitters in the inner ear |
| url |
http://hdl.handle.net/20.500.12110/paper_10447431_v20_n4_p695_Lioudyno |
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