Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), an...
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todo:paper_10440305_v19_n12_p1841_Gholipour2023-10-03T15:58:14Z Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract Gholipour, Y. Nonami, H. Erra-Balsells, R. Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa |
| spellingShingle |
Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Gholipour, Y. Nonami, H. Erra-Balsells, R. Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| topic_facet |
Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa |
| description |
Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry. |
| format |
JOUR |
| author |
Gholipour, Y. Nonami, H. Erra-Balsells, R. |
| author_facet |
Gholipour, Y. Nonami, H. Erra-Balsells, R. |
| author_sort |
Gholipour, Y. |
| title |
Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| title_short |
Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| title_full |
Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| title_fullStr |
Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| title_full_unstemmed |
Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract |
| title_sort |
application of pressure probe and uv-maldi-tof ms for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract |
| url |
http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour |
| work_keys_str_mv |
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