Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients
SUMMARY: Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression o...
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todo:paper_1040452X_v81_n8_p748_Scotti2023-10-03T15:57:53Z Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients Scotti, L. Parborell, F. Irusta, G. De Zuñiga, I. Bisioli, C. Pettorossi, H. Tesone, M. Abramovich, D. angiopoietin 1 angiopoietin 2 angiopoietin receptor beta catenin claudin 5 estradiol platelet derived growth factor platelet derived growth factor BB platelet derived growth factor DD progesterone protein ZO1 unclassified drug vascular endothelial cadherin vasculotropin angiopoietin 1 angiopoietin 2 ANGPT1 protein, human ANGPT2 protein, human claudin 5 CLDN5 protein, human estradiol platelet derived growth factor B platelet-derived growth factor BB progesterone vasculotropin A adult article blood vessel permeability cell junction clinical article cohort analysis comparative study controlled study endothelium cell enzyme linked immunosorbent assay estradiol blood level female human infertility therapy oocyte ovary follicle fluid ovary polycystic disease priority journal progesterone blood level protein blood level protein expression Western blotting metabolism Ovarian Hyperstimulation Syndrome ovary ovary follicle fluid ovary polycystic disease radioimmunoassay tight junction Adherens Junctions Adult Angiopoietin-1 Angiopoietin-2 Blotting, Western Claudin-5 Endothelial Cells Estradiol Female Follicular Fluid Humans Ovarian Hyperstimulation Syndrome Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-sis Radioimmunoassay Reproductive Techniques, Assisted Tight Junctions Vascular Endothelial Growth Factor A SUMMARY: Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growth factor (VEGF) are increased in PCOS patients. In this study, we examined the protein levels of platelet-derived growth factor (PDGF) BB and DD (PDGFBB and PDGFDD), angiopoietin 1 and 2 (ANGPT1 and ANGPT2), and their soluble receptor sTIE2 in FF from PCOS and control patients undergoing assisted reproductive techniques. We also analyzed the effect of FF from PCOS and control patients on tight and adherens junction protein expression in an endothelial cell line. PDGFBB and PDGFDD were significantly lower whereas ANGPT1 concentration was significantly higher in FF from PCOS patients than from control patients. No changes were found in the concentration of ANGPT2 or sTIE2. Expression of claudin-5 was significantly increased in endothelial cells incubated for 24hr in the presence of FF from PCOS versus from control patients, while vascular-endothelial cadherin, β-catenin, and zonula occludens 1 expression were unchanged. The changes observed in the levels of PDGF isoforms and ANGPT1 may prevent VEGF-induced vascular permeability in the PCOS ovary by regulating endothelial-cell-junction protein levels. Restoring the levels of angiogenic factors may provide new insights into PCOS treatment and the prevention of ovarian hyperstimulation syndrome in affected women. Mol. Reprod. Dev. 81: 748-756, 2014. © 2014 Wiley Periodicals, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v81_n8_p748_Scotti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiopoietin 1 angiopoietin 2 angiopoietin receptor beta catenin claudin 5 estradiol platelet derived growth factor platelet derived growth factor BB platelet derived growth factor DD progesterone protein ZO1 unclassified drug vascular endothelial cadherin vasculotropin angiopoietin 1 angiopoietin 2 ANGPT1 protein, human ANGPT2 protein, human claudin 5 CLDN5 protein, human estradiol platelet derived growth factor B platelet-derived growth factor BB progesterone vasculotropin A adult article blood vessel permeability cell junction clinical article cohort analysis comparative study controlled study endothelium cell enzyme linked immunosorbent assay estradiol blood level female human infertility therapy oocyte ovary follicle fluid ovary polycystic disease priority journal progesterone blood level protein blood level protein expression Western blotting metabolism Ovarian Hyperstimulation Syndrome ovary ovary follicle fluid ovary polycystic disease radioimmunoassay tight junction Adherens Junctions Adult Angiopoietin-1 Angiopoietin-2 Blotting, Western Claudin-5 Endothelial Cells Estradiol Female Follicular Fluid Humans Ovarian Hyperstimulation Syndrome Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-sis Radioimmunoassay Reproductive Techniques, Assisted Tight Junctions Vascular Endothelial Growth Factor A |
| spellingShingle |
angiopoietin 1 angiopoietin 2 angiopoietin receptor beta catenin claudin 5 estradiol platelet derived growth factor platelet derived growth factor BB platelet derived growth factor DD progesterone protein ZO1 unclassified drug vascular endothelial cadherin vasculotropin angiopoietin 1 angiopoietin 2 ANGPT1 protein, human ANGPT2 protein, human claudin 5 CLDN5 protein, human estradiol platelet derived growth factor B platelet-derived growth factor BB progesterone vasculotropin A adult article blood vessel permeability cell junction clinical article cohort analysis comparative study controlled study endothelium cell enzyme linked immunosorbent assay estradiol blood level female human infertility therapy oocyte ovary follicle fluid ovary polycystic disease priority journal progesterone blood level protein blood level protein expression Western blotting metabolism Ovarian Hyperstimulation Syndrome ovary ovary follicle fluid ovary polycystic disease radioimmunoassay tight junction Adherens Junctions Adult Angiopoietin-1 Angiopoietin-2 Blotting, Western Claudin-5 Endothelial Cells Estradiol Female Follicular Fluid Humans Ovarian Hyperstimulation Syndrome Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-sis Radioimmunoassay Reproductive Techniques, Assisted Tight Junctions Vascular Endothelial Growth Factor A Scotti, L. Parborell, F. Irusta, G. De Zuñiga, I. Bisioli, C. Pettorossi, H. Tesone, M. Abramovich, D. Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| topic_facet |
angiopoietin 1 angiopoietin 2 angiopoietin receptor beta catenin claudin 5 estradiol platelet derived growth factor platelet derived growth factor BB platelet derived growth factor DD progesterone protein ZO1 unclassified drug vascular endothelial cadherin vasculotropin angiopoietin 1 angiopoietin 2 ANGPT1 protein, human ANGPT2 protein, human claudin 5 CLDN5 protein, human estradiol platelet derived growth factor B platelet-derived growth factor BB progesterone vasculotropin A adult article blood vessel permeability cell junction clinical article cohort analysis comparative study controlled study endothelium cell enzyme linked immunosorbent assay estradiol blood level female human infertility therapy oocyte ovary follicle fluid ovary polycystic disease priority journal progesterone blood level protein blood level protein expression Western blotting metabolism Ovarian Hyperstimulation Syndrome ovary ovary follicle fluid ovary polycystic disease radioimmunoassay tight junction Adherens Junctions Adult Angiopoietin-1 Angiopoietin-2 Blotting, Western Claudin-5 Endothelial Cells Estradiol Female Follicular Fluid Humans Ovarian Hyperstimulation Syndrome Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-sis Radioimmunoassay Reproductive Techniques, Assisted Tight Junctions Vascular Endothelial Growth Factor A |
| description |
SUMMARY: Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growth factor (VEGF) are increased in PCOS patients. In this study, we examined the protein levels of platelet-derived growth factor (PDGF) BB and DD (PDGFBB and PDGFDD), angiopoietin 1 and 2 (ANGPT1 and ANGPT2), and their soluble receptor sTIE2 in FF from PCOS and control patients undergoing assisted reproductive techniques. We also analyzed the effect of FF from PCOS and control patients on tight and adherens junction protein expression in an endothelial cell line. PDGFBB and PDGFDD were significantly lower whereas ANGPT1 concentration was significantly higher in FF from PCOS patients than from control patients. No changes were found in the concentration of ANGPT2 or sTIE2. Expression of claudin-5 was significantly increased in endothelial cells incubated for 24hr in the presence of FF from PCOS versus from control patients, while vascular-endothelial cadherin, β-catenin, and zonula occludens 1 expression were unchanged. The changes observed in the levels of PDGF isoforms and ANGPT1 may prevent VEGF-induced vascular permeability in the PCOS ovary by regulating endothelial-cell-junction protein levels. Restoring the levels of angiogenic factors may provide new insights into PCOS treatment and the prevention of ovarian hyperstimulation syndrome in affected women. Mol. Reprod. Dev. 81: 748-756, 2014. © 2014 Wiley Periodicals, Inc. |
| format |
JOUR |
| author |
Scotti, L. Parborell, F. Irusta, G. De Zuñiga, I. Bisioli, C. Pettorossi, H. Tesone, M. Abramovich, D. |
| author_facet |
Scotti, L. Parborell, F. Irusta, G. De Zuñiga, I. Bisioli, C. Pettorossi, H. Tesone, M. Abramovich, D. |
| author_sort |
Scotti, L. |
| title |
Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| title_short |
Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| title_full |
Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| title_fullStr |
Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| title_full_unstemmed |
Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| title_sort |
platelet-derived growth factor bb and dd and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients |
| url |
http://hdl.handle.net/20.500.12110/paper_1040452X_v81_n8_p748_Scotti |
| work_keys_str_mv |
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