Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis

Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicula...

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Detalles Bibliográficos
Autores principales: Parborell, F., Dain, L., Tesone, M.
Formato: JOUR
Materias:
Apoptosis
Early antral follicles
GnRH
Growth factors
Ovary
diethylstilbestrol
DNA fragment
follitropin
gonadorelin agonist
growth factor
leuprorelin
animal cell
apoptosis
article
cell culture
controlled study
DNA cleavage
female
in vitro study
nonhuman
ovary development
ovary follicle
prepuberty
priority journal
rat
Animals
Bucladesine
Cells, Cultured
Culture Media, Serum-Free
Diethylstilbestrol
DNA Fragmentation
Epidermal Growth Factor
Estrogens, Non-Steroidal
Female
Fertility Agents, Female
Fibroblast Growth Factors
Follicle Stimulating Hormone
Follicular Atresia
Gonadotropin-Releasing Hormone
Insulin-Like Growth Factor I
Leuprolide
Ovarian Follicle
Rats
Rats, Sprague-Dawley
Sexual Maturation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_1040452X_v60_n2_p241_Parborell2023-10-03T15:57:49Z Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis Parborell, F. Dain, L. Tesone, M. Apoptosis Early antral follicles GnRH Growth factors Ovary diethylstilbestrol DNA fragment follitropin gonadorelin agonist growth factor leuprorelin animal cell apoptosis article cell culture controlled study DNA cleavage female in vitro study nonhuman ovary development ovary follicle prepuberty priority journal rat Animals Apoptosis Bucladesine Cells, Cultured Culture Media, Serum-Free Diethylstilbestrol DNA Fragmentation Epidermal Growth Factor Estrogens, Non-Steroidal Female Fertility Agents, Female Fibroblast Growth Factors Follicle Stimulating Hormone Follicular Atresia Gonadotropin-Releasing Hormone Insulin-Like Growth Factor I Leuprolide Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES-treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75-85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1-100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF-I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. © 2001 Wiley-Liss, Inc. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Apoptosis
Early antral follicles
GnRH
Growth factors
Ovary
diethylstilbestrol
DNA fragment
follitropin
gonadorelin agonist
growth factor
leuprorelin
animal cell
apoptosis
article
cell culture
controlled study
DNA cleavage
female
in vitro study
nonhuman
ovary development
ovary follicle
prepuberty
priority journal
rat
Animals
Apoptosis
Bucladesine
Cells, Cultured
Culture Media, Serum-Free
Diethylstilbestrol
DNA Fragmentation
Epidermal Growth Factor
Estrogens, Non-Steroidal
Female
Fertility Agents, Female
Fibroblast Growth Factors
Follicle Stimulating Hormone
Follicular Atresia
Gonadotropin-Releasing Hormone
Insulin-Like Growth Factor I
Leuprolide
Ovarian Follicle
Rats
Rats, Sprague-Dawley
Sexual Maturation
spellingShingle Apoptosis
Early antral follicles
GnRH
Growth factors
Ovary
diethylstilbestrol
DNA fragment
follitropin
gonadorelin agonist
growth factor
leuprorelin
animal cell
apoptosis
article
cell culture
controlled study
DNA cleavage
female
in vitro study
nonhuman
ovary development
ovary follicle
prepuberty
priority journal
rat
Animals
Apoptosis
Bucladesine
Cells, Cultured
Culture Media, Serum-Free
Diethylstilbestrol
DNA Fragmentation
Epidermal Growth Factor
Estrogens, Non-Steroidal
Female
Fertility Agents, Female
Fibroblast Growth Factors
Follicle Stimulating Hormone
Follicular Atresia
Gonadotropin-Releasing Hormone
Insulin-Like Growth Factor I
Leuprolide
Ovarian Follicle
Rats
Rats, Sprague-Dawley
Sexual Maturation
Parborell, F.
Dain, L.
Tesone, M.
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
topic_facet Apoptosis
Early antral follicles
GnRH
Growth factors
Ovary
diethylstilbestrol
DNA fragment
follitropin
gonadorelin agonist
growth factor
leuprorelin
animal cell
apoptosis
article
cell culture
controlled study
DNA cleavage
female
in vitro study
nonhuman
ovary development
ovary follicle
prepuberty
priority journal
rat
Animals
Apoptosis
Bucladesine
Cells, Cultured
Culture Media, Serum-Free
Diethylstilbestrol
DNA Fragmentation
Epidermal Growth Factor
Estrogens, Non-Steroidal
Female
Fertility Agents, Female
Fibroblast Growth Factors
Follicle Stimulating Hormone
Follicular Atresia
Gonadotropin-Releasing Hormone
Insulin-Like Growth Factor I
Leuprolide
Ovarian Follicle
Rats
Rats, Sprague-Dawley
Sexual Maturation
description Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES-treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75-85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1-100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF-I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. © 2001 Wiley-Liss, Inc.
format JOUR
author Parborell, F.
Dain, L.
Tesone, M.
author_facet Parborell, F.
Dain, L.
Tesone, M.
author_sort Parborell, F.
title Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
title_short Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
title_full Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
title_fullStr Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
title_full_unstemmed Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
title_sort gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with fsh and growth factors on the prevention of apoptosis
url http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell
work_keys_str_mv AT parborellf gonadotropinreleasinghormoneagonistaffectsratovarianfollicledevelopmentbyinterferingwithfshandgrowthfactorsonthepreventionofapoptosis
AT dainl gonadotropinreleasinghormoneagonistaffectsratovarianfollicledevelopmentbyinterferingwithfshandgrowthfactorsonthepreventionofapoptosis
AT tesonem gonadotropinreleasinghormoneagonistaffectsratovarianfollicledevelopmentbyinterferingwithfshandgrowthfactorsonthepreventionofapoptosis
_version_ 1782026657073201152

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