Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis

Gonadotropin-releasing hormone (GnRH) has been found to be expressed within the ovary and to modulate cell differentiation in ovarian cells. In the present study we have analyzed the influence of GnRH on DNA synthesis in rat granulosa cells. Cells were obtained from immature DES-treated rats and cul...

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Detalles Bibliográficos
Autores principales: Saragüeta, P.E., Lanuza, G.M., Barañao, J.L.
Formato: JOUR
Materias:
DNA synthesis
gonadotropin releasing hormone
granulosa cell
LHRH
rat
gonadorelin
animal cell
article
cell proliferation
female
gonadorelin release
growth regulation
nonhuman
ovary follicle development
priority journal
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1040452X_v47_n2_p170_Saragueta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_1040452X_v47_n2_p170_Saragueta
record_format dspace
spelling todo:paper_1040452X_v47_n2_p170_Saragueta2023-10-03T15:57:48Z Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis Saragüeta, P.E. Lanuza, G.M. Barañao, J.L. DNA synthesis gonadotropin releasing hormone granulosa cell LHRH rat gonadorelin animal cell article cell proliferation DNA synthesis female gonadorelin release granulosa cell growth regulation nonhuman ovary follicle development priority journal rat Animalia Gonadotropin-releasing hormone (GnRH) has been found to be expressed within the ovary and to modulate cell differentiation in ovarian cells. In the present study we have analyzed the influence of GnRH on DNA synthesis in rat granulosa cells. Cells were obtained from immature DES-treated rats and cultured in defined medium (DMEM:F12) containing combinations of FSH, estradiol, and transforming growth factor-β (TGF-β), both in the presence and absence of GnRH. A GnRH analog, Leuprolide (GnRHa), caused a dose- dependent inhibition of 3H-thymidine incorporation in cells cultured in the presence of FSH (20 ng/ml) and TGFβ (2.5 ng/ml), at concentrations as low as 5 x 10-11 M. Similarly, a complete inhibition of hormonally stimulated DNA synthesis were observed with another analog (Buserelin, ED50 = 1.58 ± 0.22 x 10-10 M) and native GnRH (ED50 = 1.4 ± 0.3 x 10-6 M). A competitive antagonist of GnRH (Antide) was used to neutralize the GnRH agonist effects. Antide 10-8 M could prevent the inhibition elicited by 10-7 M of Leuprolide. These results suggest that GnRH may play a role in the regulation of rat granulosa cell proliferation during follicular development. Fil:Saragüeta, P.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v47_n2_p170_Saragueta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic DNA synthesis
gonadotropin releasing hormone
granulosa cell
LHRH
rat
gonadorelin
animal cell
article
cell proliferation
DNA synthesis
female
gonadorelin release
granulosa cell
growth regulation
nonhuman
ovary follicle development
priority journal
rat
Animalia
spellingShingle DNA synthesis
gonadotropin releasing hormone
granulosa cell
LHRH
rat
gonadorelin
animal cell
article
cell proliferation
DNA synthesis
female
gonadorelin release
granulosa cell
growth regulation
nonhuman
ovary follicle development
priority journal
rat
Animalia
Saragüeta, P.E.
Lanuza, G.M.
Barañao, J.L.
Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
topic_facet DNA synthesis
gonadotropin releasing hormone
granulosa cell
LHRH
rat
gonadorelin
animal cell
article
cell proliferation
DNA synthesis
female
gonadorelin release
granulosa cell
growth regulation
nonhuman
ovary follicle development
priority journal
rat
Animalia
description Gonadotropin-releasing hormone (GnRH) has been found to be expressed within the ovary and to modulate cell differentiation in ovarian cells. In the present study we have analyzed the influence of GnRH on DNA synthesis in rat granulosa cells. Cells were obtained from immature DES-treated rats and cultured in defined medium (DMEM:F12) containing combinations of FSH, estradiol, and transforming growth factor-β (TGF-β), both in the presence and absence of GnRH. A GnRH analog, Leuprolide (GnRHa), caused a dose- dependent inhibition of 3H-thymidine incorporation in cells cultured in the presence of FSH (20 ng/ml) and TGFβ (2.5 ng/ml), at concentrations as low as 5 x 10-11 M. Similarly, a complete inhibition of hormonally stimulated DNA synthesis were observed with another analog (Buserelin, ED50 = 1.58 ± 0.22 x 10-10 M) and native GnRH (ED50 = 1.4 ± 0.3 x 10-6 M). A competitive antagonist of GnRH (Antide) was used to neutralize the GnRH agonist effects. Antide 10-8 M could prevent the inhibition elicited by 10-7 M of Leuprolide. These results suggest that GnRH may play a role in the regulation of rat granulosa cell proliferation during follicular development.
format JOUR
author Saragüeta, P.E.
Lanuza, G.M.
Barañao, J.L.
author_facet Saragüeta, P.E.
Lanuza, G.M.
Barañao, J.L.
author_sort Saragüeta, P.E.
title Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
title_short Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
title_full Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
title_fullStr Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
title_full_unstemmed Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis
title_sort inhibitory effect of gonadotrophin-releasing hormone (gnrh) on rat granulosa cell deoxyribonucleic acid synthesis
url http://hdl.handle.net/20.500.12110/paper_1040452X_v47_n2_p170_Saragueta
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AT lanuzagm inhibitoryeffectofgonadotrophinreleasinghormonegnrhonratgranulosacelldeoxyribonucleicacidsynthesis
AT baranaojl inhibitoryeffectofgonadotrophinreleasinghormonegnrhonratgranulosacelldeoxyribonucleicacidsynthesis
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