Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells
The factors involved in the inhibition of ovarian follicular cellular growth after the luteinizing hormone (LH) surge are poorly established. The aim of this study was to investigate the production of an inhibitory growth factor by human ovarian cells. Luteinized granulosa cells were obtained from a...
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todo:paper_1040452X_v36_n2_p159_Dain2023-10-03T15:57:46Z Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells Dain, L.B. Bley, M.A. Barañao, J.L. Tesone, M. Follicular proliferation Growth factor Ovary estradiol follitropin growth factor luteinizing hormone article controlled study culture medium dna synthesis female granulosa cell hormone action human human cell ovary follicle development priority journal protein analysis protein synthesis Animal Blood Physiology Cell Division Cells, Cultured Corpus Luteum Culture Media, Conditioned Culture Media, Serum-Free DNA Replication Estradiol Female Follicle Stimulating Hormone Granulosa Cells Growth Inhibitors Human Luteinizing Hormone Rats Support, Non-U.S. Gov't Ultrafiltration The factors involved in the inhibition of ovarian follicular cellular growth after the luteinizing hormone (LH) surge are poorly established. The aim of this study was to investigate the production of an inhibitory growth factor by human ovarian cells. Luteinized granulosa cells were obtained from an assisted fertilization program and were cultured in the presence or absence of follicle‐stimulating hormone (FSH) and estradiol. Data obtained by cell counting showed that the number of human luteinized granulosa cells cultured in the presence of fetal bovine serum (10%) increased 1.8‐fold within a 2‐day period. In serum‐free medium, human luteinized granulosa cells were able to incorporate 3H‐thymidine, measured during consecutive 48 h periods. During all the periods tested (up to 7 days), low basal levels of thymidine incorporation were measured and were further reduced in the presence of FSH (200 ng/ml) and estradiol (500 ng/ml). To elucidate the possible production of an inhibitory growth factor, 3H‐thymidine incorporation by rat granulosa cell cultures was measured in the presence of conditioned media (CM; from human granulosa cell cultures). In this system, FSH and estradiol elicited a tenfold increase in thymidine incorporation. The addition of CM (10% v/v collected on day 2) to FSH‐ and estradiol‐treated granulosa cell cultures produced an inhibition (61%) of thymidine incorporation. The active factor in CM withstood freeze‐thawing, was stable for several weeks at – 20°C, became unstable at 4°C, and was heat labile and sensitive to proteolysis. Ultrafiltration using membranes with different molecular weight cutoffs suggested that the factor had a molecular weight >30,000 dalton. We suggest that an inhibitory growth factor produced by human luteinized granulosa cells could be involved in the differentiation of growing follicles to corpus luteum. © 1993 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc. Fil:Bley, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v36_n2_p159_Dain |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Follicular proliferation Growth factor Ovary estradiol follitropin growth factor luteinizing hormone article controlled study culture medium dna synthesis female granulosa cell hormone action human human cell ovary follicle development priority journal protein analysis protein synthesis Animal Blood Physiology Cell Division Cells, Cultured Corpus Luteum Culture Media, Conditioned Culture Media, Serum-Free DNA Replication Estradiol Female Follicle Stimulating Hormone Granulosa Cells Growth Inhibitors Human Luteinizing Hormone Rats Support, Non-U.S. Gov't Ultrafiltration |
| spellingShingle |
Follicular proliferation Growth factor Ovary estradiol follitropin growth factor luteinizing hormone article controlled study culture medium dna synthesis female granulosa cell hormone action human human cell ovary follicle development priority journal protein analysis protein synthesis Animal Blood Physiology Cell Division Cells, Cultured Corpus Luteum Culture Media, Conditioned Culture Media, Serum-Free DNA Replication Estradiol Female Follicle Stimulating Hormone Granulosa Cells Growth Inhibitors Human Luteinizing Hormone Rats Support, Non-U.S. Gov't Ultrafiltration Dain, L.B. Bley, M.A. Barañao, J.L. Tesone, M. Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| topic_facet |
Follicular proliferation Growth factor Ovary estradiol follitropin growth factor luteinizing hormone article controlled study culture medium dna synthesis female granulosa cell hormone action human human cell ovary follicle development priority journal protein analysis protein synthesis Animal Blood Physiology Cell Division Cells, Cultured Corpus Luteum Culture Media, Conditioned Culture Media, Serum-Free DNA Replication Estradiol Female Follicle Stimulating Hormone Granulosa Cells Growth Inhibitors Human Luteinizing Hormone Rats Support, Non-U.S. Gov't Ultrafiltration |
| description |
The factors involved in the inhibition of ovarian follicular cellular growth after the luteinizing hormone (LH) surge are poorly established. The aim of this study was to investigate the production of an inhibitory growth factor by human ovarian cells. Luteinized granulosa cells were obtained from an assisted fertilization program and were cultured in the presence or absence of follicle‐stimulating hormone (FSH) and estradiol. Data obtained by cell counting showed that the number of human luteinized granulosa cells cultured in the presence of fetal bovine serum (10%) increased 1.8‐fold within a 2‐day period. In serum‐free medium, human luteinized granulosa cells were able to incorporate 3H‐thymidine, measured during consecutive 48 h periods. During all the periods tested (up to 7 days), low basal levels of thymidine incorporation were measured and were further reduced in the presence of FSH (200 ng/ml) and estradiol (500 ng/ml). To elucidate the possible production of an inhibitory growth factor, 3H‐thymidine incorporation by rat granulosa cell cultures was measured in the presence of conditioned media (CM; from human granulosa cell cultures). In this system, FSH and estradiol elicited a tenfold increase in thymidine incorporation. The addition of CM (10% v/v collected on day 2) to FSH‐ and estradiol‐treated granulosa cell cultures produced an inhibition (61%) of thymidine incorporation. The active factor in CM withstood freeze‐thawing, was stable for several weeks at – 20°C, became unstable at 4°C, and was heat labile and sensitive to proteolysis. Ultrafiltration using membranes with different molecular weight cutoffs suggested that the factor had a molecular weight >30,000 dalton. We suggest that an inhibitory growth factor produced by human luteinized granulosa cells could be involved in the differentiation of growing follicles to corpus luteum. © 1993 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc. |
| format |
JOUR |
| author |
Dain, L.B. Bley, M.A. Barañao, J.L. Tesone, M. |
| author_facet |
Dain, L.B. Bley, M.A. Barañao, J.L. Tesone, M. |
| author_sort |
Dain, L.B. |
| title |
Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| title_short |
Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| title_full |
Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| title_fullStr |
Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| title_full_unstemmed |
Evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| title_sort |
evidence for the production of a growth‐inhibitory factor by human granulosa‐luteal cells |
| url |
http://hdl.handle.net/20.500.12110/paper_1040452X_v36_n2_p159_Dain |
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