Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis
Objective: In the present work we studied the association of histamine receptors with second messengers during multistage carcinogenesis in Sencar mice skin. Methods: 96 Sencar female mouse, divided into six groups were used. Tumors appeared only in the 7,12-dimethyl-benz[a]anthracene-initiated and...
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todo:paper_10233830_v46_n8_p292_Fitzsimons2023-10-03T15:56:48Z Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis Fitzsimons, C. Molinari, B. Duran, H. Palmieri, M. Davio, C. Cricco, G. Bergoc, R. Rivera, E. cAMP Histamine receptors Inositol phosphates Mouse skin carcinogenesis 7,12 dimethylbenz[a]anthracene acetone cyclic amp histamine histamine agonist histamine h1 receptor histamine h2 receptor histamine receptor inositol phosphate phorbol 13 acetate 12 myristate phosphatidylinositol cimetidine cyclic AMP drug derivative histamine H1 receptor histamine H1 receptor antagonist histamine H2 receptor histamine H2 receptor antagonist mepyramine phosphatidylinositol tiotidine animal cell animal experiment animal model article cellular distribution controlled study drug hydrolysis female mast cell mouse nonhuman phenotype second messenger signal transduction skin carcinogenesis tumor growth animal cell count chemically induced disorder hydrolysis metabolism pathology pathophysiology physiology skin tumor 9,10-Dimethyl-1,2-benzanthracene Animals Cell Count Cimetidine Cyclic AMP Female Histamine H1 Antagonists Histamine H2 Antagonists Hydrolysis Mast Cells Mice Phosphatidylinositols Pyrilamine Receptors, Histamine H1 Receptors, Histamine H2 Second Messenger Systems Signal Transduction Skin Neoplasms Tetradecanoylphorbol Acetate Objective: In the present work we studied the association of histamine receptors with second messengers during multistage carcinogenesis in Sencar mice skin. Methods: 96 Sencar female mouse, divided into six groups were used. Tumors appeared only in the 7,12-dimethyl-benz[a]anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted group. Control groups received only TPA, or acetone or no treatment at all. Periodically during the promotion period, cAMP and inositol phosphate production were measured after stimulation with H1 or H2 agonists in samples from all groups. Results: In non-treated skin, H1 receptors were coupled to phosphatidylinositol hydrolysis and H2 receptors mediated cAMP production. Conversely, in tumors H2 receptors were associated with phosphatidylinositol hydrolysis and H1 mediated a rise in cAMP levels. The skin among tumors and the skin from all control groups maintained the same coupling as non-treated skin. An increase in mast cell number, with a homogeneous subepithelial distribution and marked phenotypic changes, was also observed in promoted skin. Conclusions: These findings indicate an atypical association of histamine receptors with second messengers that could be a critical feature for the postulated action of histamine in tumor growth. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10233830_v46_n8_p292_Fitzsimons |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP Histamine receptors Inositol phosphates Mouse skin carcinogenesis 7,12 dimethylbenz[a]anthracene acetone cyclic amp histamine histamine agonist histamine h1 receptor histamine h2 receptor histamine receptor inositol phosphate phorbol 13 acetate 12 myristate phosphatidylinositol cimetidine cyclic AMP drug derivative histamine H1 receptor histamine H1 receptor antagonist histamine H2 receptor histamine H2 receptor antagonist mepyramine phosphatidylinositol tiotidine animal cell animal experiment animal model article cellular distribution controlled study drug hydrolysis female mast cell mouse nonhuman phenotype second messenger signal transduction skin carcinogenesis tumor growth animal cell count chemically induced disorder hydrolysis metabolism pathology pathophysiology physiology skin tumor 9,10-Dimethyl-1,2-benzanthracene Animals Cell Count Cimetidine Cyclic AMP Female Histamine H1 Antagonists Histamine H2 Antagonists Hydrolysis Mast Cells Mice Phosphatidylinositols Pyrilamine Receptors, Histamine H1 Receptors, Histamine H2 Second Messenger Systems Signal Transduction Skin Neoplasms Tetradecanoylphorbol Acetate |
| spellingShingle |
cAMP Histamine receptors Inositol phosphates Mouse skin carcinogenesis 7,12 dimethylbenz[a]anthracene acetone cyclic amp histamine histamine agonist histamine h1 receptor histamine h2 receptor histamine receptor inositol phosphate phorbol 13 acetate 12 myristate phosphatidylinositol cimetidine cyclic AMP drug derivative histamine H1 receptor histamine H1 receptor antagonist histamine H2 receptor histamine H2 receptor antagonist mepyramine phosphatidylinositol tiotidine animal cell animal experiment animal model article cellular distribution controlled study drug hydrolysis female mast cell mouse nonhuman phenotype second messenger signal transduction skin carcinogenesis tumor growth animal cell count chemically induced disorder hydrolysis metabolism pathology pathophysiology physiology skin tumor 9,10-Dimethyl-1,2-benzanthracene Animals Cell Count Cimetidine Cyclic AMP Female Histamine H1 Antagonists Histamine H2 Antagonists Hydrolysis Mast Cells Mice Phosphatidylinositols Pyrilamine Receptors, Histamine H1 Receptors, Histamine H2 Second Messenger Systems Signal Transduction Skin Neoplasms Tetradecanoylphorbol Acetate Fitzsimons, C. Molinari, B. Duran, H. Palmieri, M. Davio, C. Cricco, G. Bergoc, R. Rivera, E. Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| topic_facet |
cAMP Histamine receptors Inositol phosphates Mouse skin carcinogenesis 7,12 dimethylbenz[a]anthracene acetone cyclic amp histamine histamine agonist histamine h1 receptor histamine h2 receptor histamine receptor inositol phosphate phorbol 13 acetate 12 myristate phosphatidylinositol cimetidine cyclic AMP drug derivative histamine H1 receptor histamine H1 receptor antagonist histamine H2 receptor histamine H2 receptor antagonist mepyramine phosphatidylinositol tiotidine animal cell animal experiment animal model article cellular distribution controlled study drug hydrolysis female mast cell mouse nonhuman phenotype second messenger signal transduction skin carcinogenesis tumor growth animal cell count chemically induced disorder hydrolysis metabolism pathology pathophysiology physiology skin tumor 9,10-Dimethyl-1,2-benzanthracene Animals Cell Count Cimetidine Cyclic AMP Female Histamine H1 Antagonists Histamine H2 Antagonists Hydrolysis Mast Cells Mice Phosphatidylinositols Pyrilamine Receptors, Histamine H1 Receptors, Histamine H2 Second Messenger Systems Signal Transduction Skin Neoplasms Tetradecanoylphorbol Acetate |
| description |
Objective: In the present work we studied the association of histamine receptors with second messengers during multistage carcinogenesis in Sencar mice skin. Methods: 96 Sencar female mouse, divided into six groups were used. Tumors appeared only in the 7,12-dimethyl-benz[a]anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted group. Control groups received only TPA, or acetone or no treatment at all. Periodically during the promotion period, cAMP and inositol phosphate production were measured after stimulation with H1 or H2 agonists in samples from all groups. Results: In non-treated skin, H1 receptors were coupled to phosphatidylinositol hydrolysis and H2 receptors mediated cAMP production. Conversely, in tumors H2 receptors were associated with phosphatidylinositol hydrolysis and H1 mediated a rise in cAMP levels. The skin among tumors and the skin from all control groups maintained the same coupling as non-treated skin. An increase in mast cell number, with a homogeneous subepithelial distribution and marked phenotypic changes, was also observed in promoted skin. Conclusions: These findings indicate an atypical association of histamine receptors with second messengers that could be a critical feature for the postulated action of histamine in tumor growth. |
| format |
JOUR |
| author |
Fitzsimons, C. Molinari, B. Duran, H. Palmieri, M. Davio, C. Cricco, G. Bergoc, R. Rivera, E. |
| author_facet |
Fitzsimons, C. Molinari, B. Duran, H. Palmieri, M. Davio, C. Cricco, G. Bergoc, R. Rivera, E. |
| author_sort |
Fitzsimons, C. |
| title |
Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| title_short |
Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| title_full |
Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| title_fullStr |
Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| title_full_unstemmed |
Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| title_sort |
atypical association of h1 and h2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis |
| url |
http://hdl.handle.net/20.500.12110/paper_10233830_v46_n8_p292_Fitzsimons |
| work_keys_str_mv |
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