Immunomodulatory activity of plasmatic substances.

In order to study circulating substances which could be involved in uremic immunodeficiency, the activity of plasma and plasmatic fractions of different molecular weight MW (A and B) from 12 patients with chronic renal failure (CRF) and 12 normal subjects (N) was assayed in vitro on PHA stimulated n...

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Autores principales: Lauricella, A.M., Goldman, H.L., Massouh, E.J., Nesse, A.B.
Formato: JOUR
Materias:
phytohemagglutinin
suppressor factor
article
blood
cellular immunity
chemistry
chronic kidney failure
human
immunology
in vitro study
lymphocyte activation
molecular weight
Humans
Immunity, Cellular
Kidney Failure, Chronic
Lymphocyte Activation
Molecular Weight
Phytohemagglutinins
Suppressor Factors, Immunologic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10189068_v1_n5_p341_Lauricella
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10189068_v1_n5_p341_Lauricella
record_format dspace
spelling todo:paper_10189068_v1_n5_p341_Lauricella2023-10-03T15:56:31Z Immunomodulatory activity of plasmatic substances. Lauricella, A.M. Goldman, H.L. Massouh, E.J. Nesse, A.B. phytohemagglutinin suppressor factor article blood cellular immunity chemistry chronic kidney failure human immunology in vitro study lymphocyte activation molecular weight Humans Immunity, Cellular Kidney Failure, Chronic Lymphocyte Activation Molecular Weight Phytohemagglutinins Suppressor Factors, Immunologic In order to study circulating substances which could be involved in uremic immunodeficiency, the activity of plasma and plasmatic fractions of different molecular weight MW (A and B) from 12 patients with chronic renal failure (CRF) and 12 normal subjects (N) was assayed in vitro on PHA stimulated normal lymphocyte cultures. Plasma from CRF patients inhibited lymphoproliferation compared to normal plasma activity (mean +/- SD: 9,990 +/- 3,980 cpm vs. 22,163 +/- 3,054 cpm; p < 0.001). Nevertheless, the corresponding plasmatic fractions failed to induce similar effects. Both normal fractions showed inhibitory effects on proliferation while most of the CRF fractions allowed greater cellular proliferation than the former. The dose-response curves showed that all the normal fractions contained inhibitor(s) whose effect decreased with increasing dilution. Most of the B normal fractions also produced stimulatory effects when they were diluted between 1:5 and 1:25. Variable dose-response curves were obtained in the presence of CRF fractions. However, the lack of inhibitory activity in 9 of 12 patients and the stimulatory effects produced by several A-CRF fractions suggest qualitative differences between CRF and normal fractions. Present findings demonstrate inhibitory and stimulatory activities in the normal fractions which might be due to immunomodulator substances. Disorders in this immunomodulator system could be responsible for the immunodeficiency described in CRF patients. Fil:Lauricella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10189068_v1_n5_p341_Lauricella
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic phytohemagglutinin
suppressor factor
article
blood
cellular immunity
chemistry
chronic kidney failure
human
immunology
in vitro study
lymphocyte activation
molecular weight
Humans
Immunity, Cellular
Kidney Failure, Chronic
Lymphocyte Activation
Molecular Weight
Phytohemagglutinins
Suppressor Factors, Immunologic
spellingShingle phytohemagglutinin
suppressor factor
article
blood
cellular immunity
chemistry
chronic kidney failure
human
immunology
in vitro study
lymphocyte activation
molecular weight
Humans
Immunity, Cellular
Kidney Failure, Chronic
Lymphocyte Activation
Molecular Weight
Phytohemagglutinins
Suppressor Factors, Immunologic
Lauricella, A.M.
Goldman, H.L.
Massouh, E.J.
Nesse, A.B.
Immunomodulatory activity of plasmatic substances.
topic_facet phytohemagglutinin
suppressor factor
article
blood
cellular immunity
chemistry
chronic kidney failure
human
immunology
in vitro study
lymphocyte activation
molecular weight
Humans
Immunity, Cellular
Kidney Failure, Chronic
Lymphocyte Activation
Molecular Weight
Phytohemagglutinins
Suppressor Factors, Immunologic
description In order to study circulating substances which could be involved in uremic immunodeficiency, the activity of plasma and plasmatic fractions of different molecular weight MW (A and B) from 12 patients with chronic renal failure (CRF) and 12 normal subjects (N) was assayed in vitro on PHA stimulated normal lymphocyte cultures. Plasma from CRF patients inhibited lymphoproliferation compared to normal plasma activity (mean +/- SD: 9,990 +/- 3,980 cpm vs. 22,163 +/- 3,054 cpm; p < 0.001). Nevertheless, the corresponding plasmatic fractions failed to induce similar effects. Both normal fractions showed inhibitory effects on proliferation while most of the CRF fractions allowed greater cellular proliferation than the former. The dose-response curves showed that all the normal fractions contained inhibitor(s) whose effect decreased with increasing dilution. Most of the B normal fractions also produced stimulatory effects when they were diluted between 1:5 and 1:25. Variable dose-response curves were obtained in the presence of CRF fractions. However, the lack of inhibitory activity in 9 of 12 patients and the stimulatory effects produced by several A-CRF fractions suggest qualitative differences between CRF and normal fractions. Present findings demonstrate inhibitory and stimulatory activities in the normal fractions which might be due to immunomodulator substances. Disorders in this immunomodulator system could be responsible for the immunodeficiency described in CRF patients.
format JOUR
author Lauricella, A.M.
Goldman, H.L.
Massouh, E.J.
Nesse, A.B.
author_facet Lauricella, A.M.
Goldman, H.L.
Massouh, E.J.
Nesse, A.B.
author_sort Lauricella, A.M.
title Immunomodulatory activity of plasmatic substances.
title_short Immunomodulatory activity of plasmatic substances.
title_full Immunomodulatory activity of plasmatic substances.
title_fullStr Immunomodulatory activity of plasmatic substances.
title_full_unstemmed Immunomodulatory activity of plasmatic substances.
title_sort immunomodulatory activity of plasmatic substances.
url http://hdl.handle.net/20.500.12110/paper_10189068_v1_n5_p341_Lauricella
work_keys_str_mv AT lauricellaam immunomodulatoryactivityofplasmaticsubstances
AT goldmanhl immunomodulatoryactivityofplasmaticsubstances
AT massouhej immunomodulatoryactivityofplasmaticsubstances
AT nesseab immunomodulatoryactivityofplasmaticsubstances
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