Human periprostatic adipose tissue: Its influence on prostate cancer cells
Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PC...
Guardado en:
Autores principales: | , , , , , , , , |
---|---|
Formato: | JOUR |
Materias: | |
Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca |
Aporte de: |
id |
todo:paper_10158987_v30_n1_p113_Sacca |
---|---|
record_format |
dspace |
spelling |
todo:paper_10158987_v30_n1_p113_Sacca2023-10-03T15:56:21Z Human periprostatic adipose tissue: Its influence on prostate cancer cells Sacca, P.A. Creydt, V.P. Choi, H. Mazza, O.N. Fletcher, S.J. Vallone, V.B.F. Scorticati, C. Chasseing, N.A. Calvo, J.C. Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PCa patients. The aim of this paper was to investigate the influence of factors derived from both PPATs on the behavior of androgen-dependent and castration resistant PCa cells. Methods: PPAT conditioned media (CM) were obtained from tissue samples from patients with clinically primary PCa (TPPAT) or BPH (BPPAT). Cell adhesion, proliferation, migration and metalloproteinase expression were evaluated following exposure of LNCaP (androgen dependent) and PC3 (androgen independent) prostate cancer cell lines to BPPAT or TPPAT CM. Results: Proliferation or motility of LNCaP or PC3 cells were not significantly affected by TPPAT or BPPAT CM. The number of LNCaP but not PC3 cells attached to components of TPPAT CM significantly decreased compared to cells attached to BPPAT CM. PPAT produced and released pro-MMP-9. Zymograms demonstrated that TPPAT CM induced a significant increase in pro-MMP-9 activity compared to BPPAT CM in LNCaP cells but not in PC3 cells. Conclusions: We conclude that TPPAT released factors, such as pro-MMP-9, could induce the invasive capacity of LNCaP cells and speculate that PPAT derived factors could, in the early stages of prostate cancer, modulate disease progression. © 2012 S. Karger AG, Basel. Fil:Sacca, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Creydt, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fletcher, S.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment |
spellingShingle |
Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment Sacca, P.A. Creydt, V.P. Choi, H. Mazza, O.N. Fletcher, S.J. Vallone, V.B.F. Scorticati, C. Chasseing, N.A. Calvo, J.C. Human periprostatic adipose tissue: Its influence on prostate cancer cells |
topic_facet |
Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment |
description |
Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PCa patients. The aim of this paper was to investigate the influence of factors derived from both PPATs on the behavior of androgen-dependent and castration resistant PCa cells. Methods: PPAT conditioned media (CM) were obtained from tissue samples from patients with clinically primary PCa (TPPAT) or BPH (BPPAT). Cell adhesion, proliferation, migration and metalloproteinase expression were evaluated following exposure of LNCaP (androgen dependent) and PC3 (androgen independent) prostate cancer cell lines to BPPAT or TPPAT CM. Results: Proliferation or motility of LNCaP or PC3 cells were not significantly affected by TPPAT or BPPAT CM. The number of LNCaP but not PC3 cells attached to components of TPPAT CM significantly decreased compared to cells attached to BPPAT CM. PPAT produced and released pro-MMP-9. Zymograms demonstrated that TPPAT CM induced a significant increase in pro-MMP-9 activity compared to BPPAT CM in LNCaP cells but not in PC3 cells. Conclusions: We conclude that TPPAT released factors, such as pro-MMP-9, could induce the invasive capacity of LNCaP cells and speculate that PPAT derived factors could, in the early stages of prostate cancer, modulate disease progression. © 2012 S. Karger AG, Basel. |
format |
JOUR |
author |
Sacca, P.A. Creydt, V.P. Choi, H. Mazza, O.N. Fletcher, S.J. Vallone, V.B.F. Scorticati, C. Chasseing, N.A. Calvo, J.C. |
author_facet |
Sacca, P.A. Creydt, V.P. Choi, H. Mazza, O.N. Fletcher, S.J. Vallone, V.B.F. Scorticati, C. Chasseing, N.A. Calvo, J.C. |
author_sort |
Sacca, P.A. |
title |
Human periprostatic adipose tissue: Its influence on prostate cancer cells |
title_short |
Human periprostatic adipose tissue: Its influence on prostate cancer cells |
title_full |
Human periprostatic adipose tissue: Its influence on prostate cancer cells |
title_fullStr |
Human periprostatic adipose tissue: Its influence on prostate cancer cells |
title_full_unstemmed |
Human periprostatic adipose tissue: Its influence on prostate cancer cells |
title_sort |
human periprostatic adipose tissue: its influence on prostate cancer cells |
url |
http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca |
work_keys_str_mv |
AT saccapa humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT creydtvp humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT choih humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT mazzaon humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT fletchersj humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT vallonevbf humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT scorticatic humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT chasseingna humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT calvojc humanperiprostaticadiposetissueitsinfluenceonprostatecancercells |
_version_ |
1782026313373057024 |