Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes...
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todo:paper_09697128_v4_n6_p553_Chernajovsky2023-10-03T15:55:27Z Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model Chernajovsky, Y. Adams, G. Triantaphyllopoulos, K. Ledda, M.F. Podhajcer, O.L. Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes with a retrovirus expressing TGF β1 inhibits development of arthritis in SCID mice. When DBA/1 mice, at onset of arthritis have additional arthritgenic splenocytes transferred, exacerbation occurs, reflected in a rapid increase in the number of arthritic joints, increased paw swelling and higher levels of anti-collagen antibody. By infecting arthritogenic splenocytes ex vivo with TGF β1 retrovirus, this exacerbation was inhibited. TGF β1 was effective in lowering inflammation of joints with already established arthritis and inhibiting the spreading of the disease to other joints. Transient reduction on anti-collagen antibody levels could also be obtained using purified T cells infected woth TGF β1 retrovirus. In addition, expression of TGF β1 in lymphocytes reduced the levels of gelatinase (MMP2) activity in inflamed joints. Fil:Ledda, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09697128_v4_n6_p553_Chernajovsky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus |
| spellingShingle |
Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus Chernajovsky, Y. Adams, G. Triantaphyllopoulos, K. Ledda, M.F. Podhajcer, O.L. Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| topic_facet |
Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus |
| description |
Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes with a retrovirus expressing TGF β1 inhibits development of arthritis in SCID mice. When DBA/1 mice, at onset of arthritis have additional arthritgenic splenocytes transferred, exacerbation occurs, reflected in a rapid increase in the number of arthritic joints, increased paw swelling and higher levels of anti-collagen antibody. By infecting arthritogenic splenocytes ex vivo with TGF β1 retrovirus, this exacerbation was inhibited. TGF β1 was effective in lowering inflammation of joints with already established arthritis and inhibiting the spreading of the disease to other joints. Transient reduction on anti-collagen antibody levels could also be obtained using purified T cells infected woth TGF β1 retrovirus. In addition, expression of TGF β1 in lymphocytes reduced the levels of gelatinase (MMP2) activity in inflamed joints. |
| format |
JOUR |
| author |
Chernajovsky, Y. Adams, G. Triantaphyllopoulos, K. Ledda, M.F. Podhajcer, O.L. |
| author_facet |
Chernajovsky, Y. Adams, G. Triantaphyllopoulos, K. Ledda, M.F. Podhajcer, O.L. |
| author_sort |
Chernajovsky, Y. |
| title |
Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| title_short |
Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| title_full |
Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| title_fullStr |
Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| title_full_unstemmed |
Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model |
| title_sort |
pathogenic lymphoid cells engineered to express tgf β1 ameliorate disease in a collagen-induced arthritic model |
| url |
http://hdl.handle.net/20.500.12110/paper_09697128_v4_n6_p553_Chernajovsky |
| work_keys_str_mv |
AT chernajovskyy pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel AT adamsg pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel AT triantaphyllopoulosk pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel AT leddamf pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel AT podhajcerol pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel |
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