In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.

Scutia buxifolia leaf-litter is recalcitrant to degradation but hosts an assemblage of Ascomycota fungi among which Ciliochorella sp. is the most frequent one. The aim of this work was to analyze the role Ciliochorella sp. plays in degradation of S. buxifolia leaf-litter in-vitro. Decay was assessed...

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Detalles Bibliográficos
Autores principales: Saparrat, M.C.N., Estevez, J.M., Troncozo, M.I., Arambarri, A.M., Balatti, P.A.
Formato: JOUR
Materias:
Ciliochorella sp.
Decay
FT-IR spectroscopy
Fungus
Leaf-litter
Scutia buxifolia
Aromatic structures
Endoglucanases
Fourier transform infrared
FTIR spectroscopy
Fungal growth
In-vitro
Laccases
Mass loss
Mass reduction
Peroxidase activities
Water soluble fraction
Infrared spectroscopy
Polysaccharides
Proteins
Degradation
biodegradation
dicotyledon
enzyme activity
fungus
leaf litter
lignin
microbial activity
polysaccharide
protein
Ascomycota
Fungi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09648305_v64_n3_p262_Saparrat
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09648305_v64_n3_p262_Saparrat
record_format dspace
spelling todo:paper_09648305_v64_n3_p262_Saparrat2023-10-03T15:54:53Z In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp. Saparrat, M.C.N. Estevez, J.M. Troncozo, M.I. Arambarri, A.M. Balatti, P.A. Ciliochorella sp. Decay FT-IR spectroscopy Fungus Leaf-litter Scutia buxifolia Aromatic structures Ciliochorella sp. Endoglucanases Fourier transform infrared FTIR spectroscopy Fungal growth In-vitro Laccases Mass loss Mass reduction Peroxidase activities Water soluble fraction Infrared spectroscopy Polysaccharides Proteins Degradation biodegradation dicotyledon enzyme activity FTIR spectroscopy fungus leaf litter lignin microbial activity polysaccharide protein Ascomycota Fungi Scutia buxifolia Scutia buxifolia leaf-litter is recalcitrant to degradation but hosts an assemblage of Ascomycota fungi among which Ciliochorella sp. is the most frequent one. The aim of this work was to analyze the role Ciliochorella sp. plays in degradation of S. buxifolia leaf-litter in-vitro. Decay was assessed based on mass loss and Fourier transform infrared (FT-IR) spectroscopy analysis. After 60 days of fungal growth, there was a 4.7% mass reduction that was associated with degradation of polysaccharides, proteins and lignin-related molecules. Ciliochorella sp. synthetized and released to the water-soluble fraction of leaf-litter enzymes with β-1,4 endoglucanase activity, that increased 3-fold over a 30 day period, and no laccase and peroxidase activity. We conclude that Ciliochorella sp. transformed S. buxifolia litter by degrading polysaccharides and also aromatic structures at early stages of decay. © 2010 Elsevier Ltd. All rights reserved. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09648305_v64_n3_p262_Saparrat
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ciliochorella sp.
Decay
FT-IR spectroscopy
Fungus
Leaf-litter
Scutia buxifolia
Aromatic structures
Ciliochorella sp.
Endoglucanases
Fourier transform infrared
FTIR spectroscopy
Fungal growth
In-vitro
Laccases
Mass loss
Mass reduction
Peroxidase activities
Water soluble fraction
Infrared spectroscopy
Polysaccharides
Proteins
Degradation
biodegradation
dicotyledon
enzyme activity
FTIR spectroscopy
fungus
leaf litter
lignin
microbial activity
polysaccharide
protein
Ascomycota
Fungi
Scutia buxifolia
spellingShingle Ciliochorella sp.
Decay
FT-IR spectroscopy
Fungus
Leaf-litter
Scutia buxifolia
Aromatic structures
Ciliochorella sp.
Endoglucanases
Fourier transform infrared
FTIR spectroscopy
Fungal growth
In-vitro
Laccases
Mass loss
Mass reduction
Peroxidase activities
Water soluble fraction
Infrared spectroscopy
Polysaccharides
Proteins
Degradation
biodegradation
dicotyledon
enzyme activity
FTIR spectroscopy
fungus
leaf litter
lignin
microbial activity
polysaccharide
protein
Ascomycota
Fungi
Scutia buxifolia
Saparrat, M.C.N.
Estevez, J.M.
Troncozo, M.I.
Arambarri, A.M.
Balatti, P.A.
In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
topic_facet Ciliochorella sp.
Decay
FT-IR spectroscopy
Fungus
Leaf-litter
Scutia buxifolia
Aromatic structures
Ciliochorella sp.
Endoglucanases
Fourier transform infrared
FTIR spectroscopy
Fungal growth
In-vitro
Laccases
Mass loss
Mass reduction
Peroxidase activities
Water soluble fraction
Infrared spectroscopy
Polysaccharides
Proteins
Degradation
biodegradation
dicotyledon
enzyme activity
FTIR spectroscopy
fungus
leaf litter
lignin
microbial activity
polysaccharide
protein
Ascomycota
Fungi
Scutia buxifolia
description Scutia buxifolia leaf-litter is recalcitrant to degradation but hosts an assemblage of Ascomycota fungi among which Ciliochorella sp. is the most frequent one. The aim of this work was to analyze the role Ciliochorella sp. plays in degradation of S. buxifolia leaf-litter in-vitro. Decay was assessed based on mass loss and Fourier transform infrared (FT-IR) spectroscopy analysis. After 60 days of fungal growth, there was a 4.7% mass reduction that was associated with degradation of polysaccharides, proteins and lignin-related molecules. Ciliochorella sp. synthetized and released to the water-soluble fraction of leaf-litter enzymes with β-1,4 endoglucanase activity, that increased 3-fold over a 30 day period, and no laccase and peroxidase activity. We conclude that Ciliochorella sp. transformed S. buxifolia litter by degrading polysaccharides and also aromatic structures at early stages of decay. © 2010 Elsevier Ltd. All rights reserved.
format JOUR
author Saparrat, M.C.N.
Estevez, J.M.
Troncozo, M.I.
Arambarri, A.M.
Balatti, P.A.
author_facet Saparrat, M.C.N.
Estevez, J.M.
Troncozo, M.I.
Arambarri, A.M.
Balatti, P.A.
author_sort Saparrat, M.C.N.
title In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
title_short In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
title_full In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
title_fullStr In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
title_full_unstemmed In-vitro depolymerization of Scutia buxifolia leaf-litter by a dominant Ascomycota Ciliochorella sp.
title_sort in-vitro depolymerization of scutia buxifolia leaf-litter by a dominant ascomycota ciliochorella sp.
url http://hdl.handle.net/20.500.12110/paper_09648305_v64_n3_p262_Saparrat
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