Molecular monitoring of microbial diversity in an UASB reactor

The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in th...

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Detalles Bibliográficos
Autores principales: Casserly, C., Erijman, L.
Formato: JOUR
Materias:
Bacteria
Electrophoresis
RNA
Wastewater treatment
Microbial community structure
Biodegradation
community dynamics
community structure
sludge
wastewater
Archaea
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09648305_v52_n1_p7_Casserly
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09648305_v52_n1_p7_Casserly
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spelling todo:paper_09648305_v52_n1_p7_Casserly2023-10-03T15:54:52Z Molecular monitoring of microbial diversity in an UASB reactor Casserly, C. Erijman, L. Bacteria Electrophoresis RNA Wastewater treatment Microbial community structure Biodegradation community dynamics community structure sludge wastewater Archaea Bacteria (microorganisms) The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum. © 2002 Elsevier Science Ltd. All rights reserved. Fil:Casserly, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09648305_v52_n1_p7_Casserly
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacteria
Electrophoresis
RNA
Wastewater treatment
Microbial community structure
Biodegradation
community dynamics
community structure
sludge
wastewater
Archaea
Bacteria (microorganisms)
spellingShingle Bacteria
Electrophoresis
RNA
Wastewater treatment
Microbial community structure
Biodegradation
community dynamics
community structure
sludge
wastewater
Archaea
Bacteria (microorganisms)
Casserly, C.
Erijman, L.
Molecular monitoring of microbial diversity in an UASB reactor
topic_facet Bacteria
Electrophoresis
RNA
Wastewater treatment
Microbial community structure
Biodegradation
community dynamics
community structure
sludge
wastewater
Archaea
Bacteria (microorganisms)
description The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum. © 2002 Elsevier Science Ltd. All rights reserved.
format JOUR
author Casserly, C.
Erijman, L.
author_facet Casserly, C.
Erijman, L.
author_sort Casserly, C.
title Molecular monitoring of microbial diversity in an UASB reactor
title_short Molecular monitoring of microbial diversity in an UASB reactor
title_full Molecular monitoring of microbial diversity in an UASB reactor
title_fullStr Molecular monitoring of microbial diversity in an UASB reactor
title_full_unstemmed Molecular monitoring of microbial diversity in an UASB reactor
title_sort molecular monitoring of microbial diversity in an uasb reactor
url http://hdl.handle.net/20.500.12110/paper_09648305_v52_n1_p7_Casserly
work_keys_str_mv AT casserlyc molecularmonitoringofmicrobialdiversityinanuasbreactor
AT erijmanl molecularmonitoringofmicrobialdiversityinanuasbreactor
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