Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol

To determine turgor pressure of melon and kiwifruit edible parts (Cucumis melo, L. and Actinidia deliciosa, A. Chev., respectively), we measured volume change of tissue cylinders immersed in different mannitol or polyethylene glycol 400 (PEG) solutions up to equilibrium, and sought the osmotic poten...

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Autores principales: Sajnin, C., Gerschenson, L.N., Rojas, A.M.
Formato: JOUR
Materias:
Kiwifruit
Mannitol
Melon
Polyethylene glycol
Turgor pressure
cell membrane
enzymic hydrolysis
fluorescence microscopy
macrogol
mannitol
plasmolysis
solubility
triacylglycerol lipase
turgor pressure
water flow
Actinidia deliciosa
Cucumis melo
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09639969_v32_n8_p531_Sajnin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09639969_v32_n8_p531_Sajnin
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spelling todo:paper_09639969_v32_n8_p531_Sajnin2023-10-03T15:54:35Z Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol Sajnin, C. Gerschenson, L.N. Rojas, A.M. Kiwifruit Mannitol Melon Polyethylene glycol Turgor pressure cell membrane enzymic hydrolysis fluorescence microscopy macrogol mannitol plasmolysis solubility triacylglycerol lipase turgor pressure water flow Actinidia deliciosa Cucumis melo To determine turgor pressure of melon and kiwifruit edible parts (Cucumis melo, L. and Actinidia deliciosa, A. Chev., respectively), we measured volume change of tissue cylinders immersed in different mannitol or polyethylene glycol 400 (PEG) solutions up to equilibrium, and sought the osmotic potential that led to incipient plasmolysis. Compression tests were also performed to compare the mechanical response of these tissues at different turgor conditions. Cellular vitality was evaluated through fluorescein diacetate hydrolyisis by lipases located in the active cellular membrane. This enzyme-catalyzed reaction was observed by fluorescence microscopy. Results indicated that turgor pressure of kiwifruit could only be evaluated by using PEG as a solute. Immersion in PEG solutions did not affect the mechanical response of this tissue 'per se' based on a comparison of tissue firmness. On the one hand turgor pressure values determined in melon samples were different using both solutes and on the other the immersion affected melon tissue because a significantly (P < 0.05) higher firmness was obtained for raw (unsoaked) melon cylinders. Cellular membranes were not affected by mannitol or PEG shown by fluorescence microscopy of isotonic melon samples. Some solute-specific effect could change the water movement into the tissue affecting the value of melon turgor pressure finally estimated. Thus, we emphasized the importance of using the same solute to compare the effect of different treatments and/or ripeness grade on turgor pressure when incipient plasmolysis technique is used. In this way, PEG showed to be a useful alternative solute due to its wide solubility range. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09639969_v32_n8_p531_Sajnin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Kiwifruit
Mannitol
Melon
Polyethylene glycol
Turgor pressure
cell membrane
enzymic hydrolysis
fluorescence microscopy
macrogol
mannitol
plasmolysis
solubility
triacylglycerol lipase
turgor pressure
water flow
Actinidia deliciosa
Cucumis melo
spellingShingle Kiwifruit
Mannitol
Melon
Polyethylene glycol
Turgor pressure
cell membrane
enzymic hydrolysis
fluorescence microscopy
macrogol
mannitol
plasmolysis
solubility
triacylglycerol lipase
turgor pressure
water flow
Actinidia deliciosa
Cucumis melo
Sajnin, C.
Gerschenson, L.N.
Rojas, A.M.
Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
topic_facet Kiwifruit
Mannitol
Melon
Polyethylene glycol
Turgor pressure
cell membrane
enzymic hydrolysis
fluorescence microscopy
macrogol
mannitol
plasmolysis
solubility
triacylglycerol lipase
turgor pressure
water flow
Actinidia deliciosa
Cucumis melo
description To determine turgor pressure of melon and kiwifruit edible parts (Cucumis melo, L. and Actinidia deliciosa, A. Chev., respectively), we measured volume change of tissue cylinders immersed in different mannitol or polyethylene glycol 400 (PEG) solutions up to equilibrium, and sought the osmotic potential that led to incipient plasmolysis. Compression tests were also performed to compare the mechanical response of these tissues at different turgor conditions. Cellular vitality was evaluated through fluorescein diacetate hydrolyisis by lipases located in the active cellular membrane. This enzyme-catalyzed reaction was observed by fluorescence microscopy. Results indicated that turgor pressure of kiwifruit could only be evaluated by using PEG as a solute. Immersion in PEG solutions did not affect the mechanical response of this tissue 'per se' based on a comparison of tissue firmness. On the one hand turgor pressure values determined in melon samples were different using both solutes and on the other the immersion affected melon tissue because a significantly (P < 0.05) higher firmness was obtained for raw (unsoaked) melon cylinders. Cellular membranes were not affected by mannitol or PEG shown by fluorescence microscopy of isotonic melon samples. Some solute-specific effect could change the water movement into the tissue affecting the value of melon turgor pressure finally estimated. Thus, we emphasized the importance of using the same solute to compare the effect of different treatments and/or ripeness grade on turgor pressure when incipient plasmolysis technique is used. In this way, PEG showed to be a useful alternative solute due to its wide solubility range.
format JOUR
author Sajnin, C.
Gerschenson, L.N.
Rojas, A.M.
author_facet Sajnin, C.
Gerschenson, L.N.
Rojas, A.M.
author_sort Sajnin, C.
title Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
title_short Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
title_full Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
title_fullStr Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
title_full_unstemmed Turgor pressure in vegetable tissues: Comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
title_sort turgor pressure in vegetable tissues: comparison of the performance of incipient plasmolysis technique using mannitol and polyethylenglycol
url http://hdl.handle.net/20.500.12110/paper_09639969_v32_n8_p531_Sajnin
work_keys_str_mv AT sajninc turgorpressureinvegetabletissuescomparisonoftheperformanceofincipientplasmolysistechniqueusingmannitolandpolyethylenglycol
AT gerschensonln turgorpressureinvegetabletissuescomparisonoftheperformanceofincipientplasmolysistechniqueusingmannitolandpolyethylenglycol
AT rojasam turgorpressureinvegetabletissuescomparisonoftheperformanceofincipientplasmolysistechniqueusingmannitolandpolyethylenglycol
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