Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila

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High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophil...

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Autores principales: De Panis, D.N., Padró, J., Furió-Tarí, P., Tarazona, S., Milla Carmona, P.S., Soto, I.M., Dopazo, H., Conesa, A., Hasson, E.
Formato: JOUR
Materias:
alkaloids
environment adaptation
mescaline
plasticity
RNA-Seq
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09621083_v25_n18_p4534_DePanis
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09621083_v25_n18_p4534_DePanis
record_format dspace
spelling todo:paper_09621083_v25_n18_p4534_DePanis2023-10-03T15:54:13Z Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila De Panis, D.N. Padró, J. Furió-Tarí, P. Tarazona, S. Milla Carmona, P.S. Soto, I.M. Dopazo, H. Conesa, A. Hasson, E. alkaloids environment adaptation mescaline plasticity RNA-Seq High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation–reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd Fil:Padró, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Soto, I.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dopazo, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09621083_v25_n18_p4534_DePanis
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic alkaloids
environment adaptation
mescaline
plasticity
RNA-Seq
spellingShingle alkaloids
environment adaptation
mescaline
plasticity
RNA-Seq
De Panis, D.N.
Padró, J.
Furió-Tarí, P.
Tarazona, S.
Milla Carmona, P.S.
Soto, I.M.
Dopazo, H.
Conesa, A.
Hasson, E.
Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
topic_facet alkaloids
environment adaptation
mescaline
plasticity
RNA-Seq
description High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation–reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd
format JOUR
author De Panis, D.N.
Padró, J.
Furió-Tarí, P.
Tarazona, S.
Milla Carmona, P.S.
Soto, I.M.
Dopazo, H.
Conesa, A.
Hasson, E.
author_facet De Panis, D.N.
Padró, J.
Furió-Tarí, P.
Tarazona, S.
Milla Carmona, P.S.
Soto, I.M.
Dopazo, H.
Conesa, A.
Hasson, E.
author_sort De Panis, D.N.
title Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_short Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_fullStr Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full_unstemmed Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_sort transcriptome modulation during host shift is driven by secondary metabolites in desert drosophila
url http://hdl.handle.net/20.500.12110/paper_09621083_v25_n18_p4534_DePanis
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AT hassone transcriptomemodulationduringhostshiftisdrivenbysecondarymetabolitesindesertdrosophila
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