Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases

The presence and characteristics of androgen receptors (ARs) have been described by our group in one human melanoma cell line. We have now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies from human metastatic melanoma. Scatchard...

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Autores principales:	Morvillo, V., Lüthy, I.A., Bravo, A.I., Capurro, M.I., Portela, P., Calandra, R.S., Mordoh, J.
Formato:	JOUR
Materias:	Androgen Androgen receptor Anti-androgen Anti-oestrogen Flutamide Melanoma Tamoxifen androgen androgen receptor androstanolone antiandrogen bicalutamide cell protein estradiol estrogen receptor hydroxyflutamide metribolone progesterone progesterone receptor tamoxifen testosterone accuracy article binding competition cancer cell culture cell proliferation controlled study dissociation drug effect drug receptor binding drug specificity hormone action human human cell immunocytochemistry melanoma metastasis priority journal protein binding receptor affinity Scatchard plot staining tumor biopsy Adult Binding, Competitive Biopsy Cell Division Data Interpretation, Statistical Dihydrotestosterone Female Hormones Humans Immunohistochemistry Male Melanoma, Amelanotic Receptors, Androgen Receptors, Steroid Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09608931_v12_n6_p529_Morvillo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09608931_v12_n6_p529_Morvillo
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spelling	todo:paper_09608931_v12_n6_p529_Morvillo2023-10-03T15:53:57Z Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases Morvillo, V. Lüthy, I.A. Bravo, A.I. Capurro, M.I. Portela, P. Calandra, R.S. Mordoh, J. Androgen Androgen receptor Anti-androgen Anti-oestrogen Flutamide Melanoma Tamoxifen androgen androgen receptor androstanolone antiandrogen bicalutamide cell protein estradiol estrogen receptor hydroxyflutamide metribolone progesterone progesterone receptor tamoxifen testosterone accuracy article binding competition cancer cell culture cell proliferation controlled study dissociation drug effect drug receptor binding drug specificity hormone action human human cell immunocytochemistry melanoma metastasis priority journal protein binding receptor affinity Scatchard plot staining tumor biopsy Adult Binding, Competitive Biopsy Cell Division Data Interpretation, Statistical Dihydrotestosterone Female Hormones Humans Immunohistochemistry Male Melanoma Melanoma, Amelanotic Receptors, Androgen Receptors, Steroid Tumor Cells, Cultured The presence and characteristics of androgen receptors (ARs) have been described by our group in one human melanoma cell line. We have now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies from human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein, for IIB-MEL-LES cells and 14 nM, with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN cells. When specificity was assessed, not only androgen and anti-androgen but also non-androgenic compounds were able to compete for [3H]R1881 binding, as seen before. When immunocytochemistry of IIB-MEL-LES and IIB-MEL-IAN cells was performed for ARs, both cell lines were deeply stained in the nucleus, whereas no staining was found for oestrogen or progesterone receptors. Every specimen of melanoma metastases tested for the presence of ARs was deeply stained, and in the majority the intensity of the staining was high. Several hormones and anti-hormones were tested for their ability to affect cell proliferation. In both cell lines, testosterone, dihydrotesterone, oestradiol and progesterone significantly stimulated cell proliferation, and this was reversed by hydroxyflutamide, bicalutamide or tamoxifen. © 2002 Lippincott Williams & Wilkins. Fil:Morvillo, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lüthy, I.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Capurro, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portela, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09608931_v12_n6_p529_Morvillo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Androgen Androgen receptor Anti-androgen Anti-oestrogen Flutamide Melanoma Tamoxifen androgen androgen receptor androstanolone antiandrogen bicalutamide cell protein estradiol estrogen receptor hydroxyflutamide metribolone progesterone progesterone receptor tamoxifen testosterone accuracy article binding competition cancer cell culture cell proliferation controlled study dissociation drug effect drug receptor binding drug specificity hormone action human human cell immunocytochemistry melanoma metastasis priority journal protein binding receptor affinity Scatchard plot staining tumor biopsy Adult Binding, Competitive Biopsy Cell Division Data Interpretation, Statistical Dihydrotestosterone Female Hormones Humans Immunohistochemistry Male Melanoma Melanoma, Amelanotic Receptors, Androgen Receptors, Steroid Tumor Cells, Cultured
spellingShingle	Androgen Androgen receptor Anti-androgen Anti-oestrogen Flutamide Melanoma Tamoxifen androgen androgen receptor androstanolone antiandrogen bicalutamide cell protein estradiol estrogen receptor hydroxyflutamide metribolone progesterone progesterone receptor tamoxifen testosterone accuracy article binding competition cancer cell culture cell proliferation controlled study dissociation drug effect drug receptor binding drug specificity hormone action human human cell immunocytochemistry melanoma metastasis priority journal protein binding receptor affinity Scatchard plot staining tumor biopsy Adult Binding, Competitive Biopsy Cell Division Data Interpretation, Statistical Dihydrotestosterone Female Hormones Humans Immunohistochemistry Male Melanoma Melanoma, Amelanotic Receptors, Androgen Receptors, Steroid Tumor Cells, Cultured Morvillo, V. Lüthy, I.A. Bravo, A.I. Capurro, M.I. Portela, P. Calandra, R.S. Mordoh, J. Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
topic_facet	Androgen Androgen receptor Anti-androgen Anti-oestrogen Flutamide Melanoma Tamoxifen androgen androgen receptor androstanolone antiandrogen bicalutamide cell protein estradiol estrogen receptor hydroxyflutamide metribolone progesterone progesterone receptor tamoxifen testosterone accuracy article binding competition cancer cell culture cell proliferation controlled study dissociation drug effect drug receptor binding drug specificity hormone action human human cell immunocytochemistry melanoma metastasis priority journal protein binding receptor affinity Scatchard plot staining tumor biopsy Adult Binding, Competitive Biopsy Cell Division Data Interpretation, Statistical Dihydrotestosterone Female Hormones Humans Immunohistochemistry Male Melanoma Melanoma, Amelanotic Receptors, Androgen Receptors, Steroid Tumor Cells, Cultured
description	The presence and characteristics of androgen receptors (ARs) have been described by our group in one human melanoma cell line. We have now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies from human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein, for IIB-MEL-LES cells and 14 nM, with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN cells. When specificity was assessed, not only androgen and anti-androgen but also non-androgenic compounds were able to compete for [3H]R1881 binding, as seen before. When immunocytochemistry of IIB-MEL-LES and IIB-MEL-IAN cells was performed for ARs, both cell lines were deeply stained in the nucleus, whereas no staining was found for oestrogen or progesterone receptors. Every specimen of melanoma metastases tested for the presence of ARs was deeply stained, and in the majority the intensity of the staining was high. Several hormones and anti-hormones were tested for their ability to affect cell proliferation. In both cell lines, testosterone, dihydrotesterone, oestradiol and progesterone significantly stimulated cell proliferation, and this was reversed by hydroxyflutamide, bicalutamide or tamoxifen. © 2002 Lippincott Williams & Wilkins.
format	JOUR
author	Morvillo, V. Lüthy, I.A. Bravo, A.I. Capurro, M.I. Portela, P. Calandra, R.S. Mordoh, J.
author_facet	Morvillo, V. Lüthy, I.A. Bravo, A.I. Capurro, M.I. Portela, P. Calandra, R.S. Mordoh, J.
author_sort	Morvillo, V.
title	Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_short	Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_full	Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_fullStr	Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_full_unstemmed	Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_sort	androgen receptors in human melanoma cell lines iib-mel-les and iib-mel-ian and in human melanoma metastases
url	http://hdl.handle.net/20.500.12110/paper_09608931_v12_n6_p529_Morvillo
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_version_	1807320333315735552

Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases

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