A CDPK isoform participates in the regulation of nodule number in Medicago truncatula

Medicago spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti. Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago...

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Detalles Bibliográficos
Autores principales: Gargantini, P.R., Gonzalez-Rizzo, S., Chinchilla, D., Raices, M., Giammaria, V., Ulloa, R.M., Frugier, F., Crespi, M.D.
Formato: JOUR
Materias:
CDPK
Cytokinins
Medicago truncatula
Nodulation
Stress
Cell membranes
Plants (botany)
Stresses
Calcium-dependent protein kinases (CDPK)
Symbiotic interaction
Enzymes
Cells
Plants
Agrobacterium rhizogenes
Allium cepa
Medicago
Rhizobiaceae
Sinorhizobium meliloti
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09607412_v48_n6_p843_Gargantini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09607412_v48_n6_p843_Gargantini
record_format dspace
spelling todo:paper_09607412_v48_n6_p843_Gargantini2023-10-03T15:53:49Z A CDPK isoform participates in the regulation of nodule number in Medicago truncatula Gargantini, P.R. Gonzalez-Rizzo, S. Chinchilla, D. Raices, M. Giammaria, V. Ulloa, R.M. Frugier, F. Crespi, M.D. CDPK Cytokinins Medicago truncatula Nodulation Stress Cell membranes Plants (botany) Stresses Calcium-dependent protein kinases (CDPK) Medicago truncatula Nodulation Symbiotic interaction Enzymes Cells Cytokinins Enzymes Plants Stresses Agrobacterium rhizogenes Allium cepa Medicago Medicago truncatula Rhizobiaceae Sinorhizobium meliloti Medicago spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti. Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula, is regulated during the nodulation process. Early inductions were detected 15 min and 3-4 days post-inoculation (dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatuladmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of nodule primordia, and was not observed in wild-type roots inoculated with nod- strains. This late induction correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3 antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3 participates in the regulation of the symbiotic interaction. © 2006 The Authors. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09607412_v48_n6_p843_Gargantini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic CDPK
Cytokinins
Medicago truncatula
Nodulation
Stress
Cell membranes
Plants (botany)
Stresses
Calcium-dependent protein kinases (CDPK)
Medicago truncatula
Nodulation
Symbiotic interaction
Enzymes
Cells
Cytokinins
Enzymes
Plants
Stresses
Agrobacterium rhizogenes
Allium cepa
Medicago
Medicago truncatula
Rhizobiaceae
Sinorhizobium meliloti
spellingShingle CDPK
Cytokinins
Medicago truncatula
Nodulation
Stress
Cell membranes
Plants (botany)
Stresses
Calcium-dependent protein kinases (CDPK)
Medicago truncatula
Nodulation
Symbiotic interaction
Enzymes
Cells
Cytokinins
Enzymes
Plants
Stresses
Agrobacterium rhizogenes
Allium cepa
Medicago
Medicago truncatula
Rhizobiaceae
Sinorhizobium meliloti
Gargantini, P.R.
Gonzalez-Rizzo, S.
Chinchilla, D.
Raices, M.
Giammaria, V.
Ulloa, R.M.
Frugier, F.
Crespi, M.D.
A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
topic_facet CDPK
Cytokinins
Medicago truncatula
Nodulation
Stress
Cell membranes
Plants (botany)
Stresses
Calcium-dependent protein kinases (CDPK)
Medicago truncatula
Nodulation
Symbiotic interaction
Enzymes
Cells
Cytokinins
Enzymes
Plants
Stresses
Agrobacterium rhizogenes
Allium cepa
Medicago
Medicago truncatula
Rhizobiaceae
Sinorhizobium meliloti
description Medicago spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti. Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula, is regulated during the nodulation process. Early inductions were detected 15 min and 3-4 days post-inoculation (dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatuladmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of nodule primordia, and was not observed in wild-type roots inoculated with nod- strains. This late induction correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3 antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3 participates in the regulation of the symbiotic interaction. © 2006 The Authors.
format JOUR
author Gargantini, P.R.
Gonzalez-Rizzo, S.
Chinchilla, D.
Raices, M.
Giammaria, V.
Ulloa, R.M.
Frugier, F.
Crespi, M.D.
author_facet Gargantini, P.R.
Gonzalez-Rizzo, S.
Chinchilla, D.
Raices, M.
Giammaria, V.
Ulloa, R.M.
Frugier, F.
Crespi, M.D.
author_sort Gargantini, P.R.
title A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
title_short A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
title_full A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
title_fullStr A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
title_full_unstemmed A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
title_sort cdpk isoform participates in the regulation of nodule number in medicago truncatula
url http://hdl.handle.net/20.500.12110/paper_09607412_v48_n6_p843_Gargantini
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