Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid scr...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09607412_v42_n4_p492_Kaothien |
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todo:paper_09607412_v42_n4_p492_Kaothien2023-10-03T15:53:48Z Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth Kaothien, P. Sung, H.O. Shuai, B. Wengier, D. Cotter, R. Kelley, D. Kiriakopolos, S. Muschietti, J. McCormick, S. Actin Cytoplasmic streaming Signaling Tomato Vacuole Yeast two-hybrid Bioassay Cytology Enzymes Growth kinetics Proteins Yeast Cytoplasmic domains (CD) Kinase partner protein (KPP) Pollen germination Receptor kinases Enzyme kinetics protein kinase vegetable protein article enzymology flower gene expression growth, development and aging metabolism molecular genetics nucleotide sequence protein binding protein tertiary structure signal transduction tomato transgenic plant two hybrid system Flowers Gene Expression Lycopersicon esculentum Molecular Sequence Data Plant Proteins Plants, Genetically Modified Protein Binding Protein Kinases Protein Structure, Tertiary Signal Transduction Two-Hybrid System Techniques Biological Tests Cytology Enzymatic Activity Enzymes Germination Growth Rate Pollen Proteins Yeasts Arabidopsis Arabidopsis thaliana Lycopersicon esculentum The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues. © 2005 Blackwell Publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09607412_v42_n4_p492_Kaothien |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Actin Cytoplasmic streaming Signaling Tomato Vacuole Yeast two-hybrid Bioassay Cytology Enzymes Growth kinetics Proteins Yeast Cytoplasmic domains (CD) Kinase partner protein (KPP) Pollen germination Receptor kinases Enzyme kinetics protein kinase vegetable protein article enzymology flower gene expression growth, development and aging metabolism molecular genetics nucleotide sequence protein binding protein tertiary structure signal transduction tomato transgenic plant two hybrid system Flowers Gene Expression Lycopersicon esculentum Molecular Sequence Data Plant Proteins Plants, Genetically Modified Protein Binding Protein Kinases Protein Structure, Tertiary Signal Transduction Two-Hybrid System Techniques Biological Tests Cytology Enzymatic Activity Enzymes Germination Growth Rate Pollen Proteins Yeasts Arabidopsis Arabidopsis thaliana Lycopersicon esculentum |
| spellingShingle |
Actin Cytoplasmic streaming Signaling Tomato Vacuole Yeast two-hybrid Bioassay Cytology Enzymes Growth kinetics Proteins Yeast Cytoplasmic domains (CD) Kinase partner protein (KPP) Pollen germination Receptor kinases Enzyme kinetics protein kinase vegetable protein article enzymology flower gene expression growth, development and aging metabolism molecular genetics nucleotide sequence protein binding protein tertiary structure signal transduction tomato transgenic plant two hybrid system Flowers Gene Expression Lycopersicon esculentum Molecular Sequence Data Plant Proteins Plants, Genetically Modified Protein Binding Protein Kinases Protein Structure, Tertiary Signal Transduction Two-Hybrid System Techniques Biological Tests Cytology Enzymatic Activity Enzymes Germination Growth Rate Pollen Proteins Yeasts Arabidopsis Arabidopsis thaliana Lycopersicon esculentum Kaothien, P. Sung, H.O. Shuai, B. Wengier, D. Cotter, R. Kelley, D. Kiriakopolos, S. Muschietti, J. McCormick, S. Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| topic_facet |
Actin Cytoplasmic streaming Signaling Tomato Vacuole Yeast two-hybrid Bioassay Cytology Enzymes Growth kinetics Proteins Yeast Cytoplasmic domains (CD) Kinase partner protein (KPP) Pollen germination Receptor kinases Enzyme kinetics protein kinase vegetable protein article enzymology flower gene expression growth, development and aging metabolism molecular genetics nucleotide sequence protein binding protein tertiary structure signal transduction tomato transgenic plant two hybrid system Flowers Gene Expression Lycopersicon esculentum Molecular Sequence Data Plant Proteins Plants, Genetically Modified Protein Binding Protein Kinases Protein Structure, Tertiary Signal Transduction Two-Hybrid System Techniques Biological Tests Cytology Enzymatic Activity Enzymes Germination Growth Rate Pollen Proteins Yeasts Arabidopsis Arabidopsis thaliana Lycopersicon esculentum |
| description |
The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues. © 2005 Blackwell Publishing Ltd. |
| format |
JOUR |
| author |
Kaothien, P. Sung, H.O. Shuai, B. Wengier, D. Cotter, R. Kelley, D. Kiriakopolos, S. Muschietti, J. McCormick, S. |
| author_facet |
Kaothien, P. Sung, H.O. Shuai, B. Wengier, D. Cotter, R. Kelley, D. Kiriakopolos, S. Muschietti, J. McCormick, S. |
| author_sort |
Kaothien, P. |
| title |
Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| title_short |
Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| title_full |
Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| title_fullStr |
Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| title_full_unstemmed |
Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth |
| title_sort |
kinase partner protein interacts with the leprk1 and leprk2 receptor kinases and plays a role in polarized pollen tube growth |
| url |
http://hdl.handle.net/20.500.12110/paper_09607412_v42_n4_p492_Kaothien |
| work_keys_str_mv |
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