Regulatory effects of 5β-reduced steroids
The first section of this publication summarizes early work according to which 5β-pregnanedione is an important metabolite of progesterone in the early stages of the chick embryo's adrenal steroidogenesis, then decreasing gradually as corticosteroidogenesis increases. In the second section a mo...
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todo:paper_09600760_v39_n2_p253_Aragones2023-10-03T15:53:26Z Regulatory effects of 5β-reduced steroids Aragonés, A. Gonzalez, C.B. Spinedi, N.C. Lantos, C.P. 3(or 17)beta hydroxysteroid dehydrogenase aminolevulinic acid corticosterone cytochrome p450 estradiol pregnanedione progesterone adrenal gland animal tissue chicken conference paper embryo newborn nonhuman priority journal steroidogenesis Aminolevulinic Acid Animal Chick Embryo Chickens Corticosterone Cyanoketone Cytochrome P-450 Enzyme System Estradiol Male Oxidation-Reduction Pregnanediones Progesterone Spironolactone Steroids Support, Non-U.S. Gov't The first section of this publication summarizes early work according to which 5β-pregnanedione is an important metabolite of progesterone in the early stages of the chick embryo's adrenal steroidogenesis, then decreasing gradually as corticosteroidogenesis increases. In the second section a model is described in which adrenal 3β-ol hydroxylase-isomerase of the 17-day-old chicken is suppressed pharmacologically, this suppression being correlated with that of the synthesis of aminoevulinic acid (ALA), the first and rate-limiting step of the heme pathway. 5β-Pregnanedione (10 -7 -10 -6 M) restored ALA synthesis in this inhibited model to normal values. The effect of 5β-pregnanedione was specific since other steroids tested: progesterone; 5α-pregnanedione; corticosterone or estradiol, did not stimulate ALA. Since heme formation by steroidogenic glands contributes to the synthesis of cytochrome P450 rather than hemoglobin, 5β-pregnanedione was also assayed as a stimulator of this enzyme system and was found to increase cytochrome P450 in adrenals and testes but not in the liver. In view of these results a hypothesis is advanced according to which 5β-reduced progestagens and androgens stimulate cytochrome P450 formation, i.e. the synthesis of progesterone and higher hydroxylated steroids, by steroidogenic glands in the event of an excessive precursor reduction. © 1991. Fil:Aragonés, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gonzalez, C.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spinedi, N.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09600760_v39_n2_p253_Aragones |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3(or 17)beta hydroxysteroid dehydrogenase aminolevulinic acid corticosterone cytochrome p450 estradiol pregnanedione progesterone adrenal gland animal tissue chicken conference paper embryo newborn nonhuman priority journal steroidogenesis Aminolevulinic Acid Animal Chick Embryo Chickens Corticosterone Cyanoketone Cytochrome P-450 Enzyme System Estradiol Male Oxidation-Reduction Pregnanediones Progesterone Spironolactone Steroids Support, Non-U.S. Gov't |
| spellingShingle |
3(or 17)beta hydroxysteroid dehydrogenase aminolevulinic acid corticosterone cytochrome p450 estradiol pregnanedione progesterone adrenal gland animal tissue chicken conference paper embryo newborn nonhuman priority journal steroidogenesis Aminolevulinic Acid Animal Chick Embryo Chickens Corticosterone Cyanoketone Cytochrome P-450 Enzyme System Estradiol Male Oxidation-Reduction Pregnanediones Progesterone Spironolactone Steroids Support, Non-U.S. Gov't Aragonés, A. Gonzalez, C.B. Spinedi, N.C. Lantos, C.P. Regulatory effects of 5β-reduced steroids |
| topic_facet |
3(or 17)beta hydroxysteroid dehydrogenase aminolevulinic acid corticosterone cytochrome p450 estradiol pregnanedione progesterone adrenal gland animal tissue chicken conference paper embryo newborn nonhuman priority journal steroidogenesis Aminolevulinic Acid Animal Chick Embryo Chickens Corticosterone Cyanoketone Cytochrome P-450 Enzyme System Estradiol Male Oxidation-Reduction Pregnanediones Progesterone Spironolactone Steroids Support, Non-U.S. Gov't |
| description |
The first section of this publication summarizes early work according to which 5β-pregnanedione is an important metabolite of progesterone in the early stages of the chick embryo's adrenal steroidogenesis, then decreasing gradually as corticosteroidogenesis increases. In the second section a model is described in which adrenal 3β-ol hydroxylase-isomerase of the 17-day-old chicken is suppressed pharmacologically, this suppression being correlated with that of the synthesis of aminoevulinic acid (ALA), the first and rate-limiting step of the heme pathway. 5β-Pregnanedione (10 -7 -10 -6 M) restored ALA synthesis in this inhibited model to normal values. The effect of 5β-pregnanedione was specific since other steroids tested: progesterone; 5α-pregnanedione; corticosterone or estradiol, did not stimulate ALA. Since heme formation by steroidogenic glands contributes to the synthesis of cytochrome P450 rather than hemoglobin, 5β-pregnanedione was also assayed as a stimulator of this enzyme system and was found to increase cytochrome P450 in adrenals and testes but not in the liver. In view of these results a hypothesis is advanced according to which 5β-reduced progestagens and androgens stimulate cytochrome P450 formation, i.e. the synthesis of progesterone and higher hydroxylated steroids, by steroidogenic glands in the event of an excessive precursor reduction. © 1991. |
| format |
JOUR |
| author |
Aragonés, A. Gonzalez, C.B. Spinedi, N.C. Lantos, C.P. |
| author_facet |
Aragonés, A. Gonzalez, C.B. Spinedi, N.C. Lantos, C.P. |
| author_sort |
Aragonés, A. |
| title |
Regulatory effects of 5β-reduced steroids |
| title_short |
Regulatory effects of 5β-reduced steroids |
| title_full |
Regulatory effects of 5β-reduced steroids |
| title_fullStr |
Regulatory effects of 5β-reduced steroids |
| title_full_unstemmed |
Regulatory effects of 5β-reduced steroids |
| title_sort |
regulatory effects of 5β-reduced steroids |
| url |
http://hdl.handle.net/20.500.12110/paper_09600760_v39_n2_p253_Aragones |
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AT aragonesa regulatoryeffectsof5breducedsteroids AT gonzalezcb regulatoryeffectsof5breducedsteroids AT spinedinc regulatoryeffectsof5breducedsteroids AT lantoscp regulatoryeffectsof5breducedsteroids |
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