In situ characterization of phospholipid coated electrodes

Four in situ techniques, quartz crystal microbalance, electrochemical impedance spectroscopy, ellipsometry and atomic force microscopy, have been used to study the deposition of dioleoylphosphatidylglycerol (DOPG) films from vesicle solution onto 1-decanethiol coated gold surfaces. The experiments s...

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Detalles Bibliográficos
Autores principales:	Bartlett, P.N., Brace, K., Calvo, E.J., Etchenique, R.
Formato:	JOUR
Materias:	phospholipid article crystal electrochemistry electrode film impedance ionic strength lipid analysis pH
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09599428_v10_n1_p149_Bartlett
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09599428_v10_n1_p149_Bartlett
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spelling	todo:paper_09599428_v10_n1_p149_Bartlett2023-10-03T15:53:15Z In situ characterization of phospholipid coated electrodes Bartlett, P.N. Brace, K. Calvo, E.J. Etchenique, R. phospholipid article crystal electrochemistry electrode film impedance ionic strength lipid analysis pH Four in situ techniques, quartz crystal microbalance, electrochemical impedance spectroscopy, ellipsometry and atomic force microscopy, have been used to study the deposition of dioleoylphosphatidylglycerol (DOPG) films from vesicle solution onto 1-decanethiol coated gold surfaces. The experiments show that, although no one technique can give an unequivocal picture of the deposition process and structure of the final film, by combining the results from the four different techniques a consistent picture emerges. Initially, over the first 200 s a monolayer of DOPG is formed on the 1-decanethiol coated surface. Then at longer times and in the presence of 0.1 M NaCl and 0.1 M TRIS buffer at pH 8 a further bilayer of DOPG is deposited on top of this initially formed monolayer. Evidence for this is provided by the quartz crystal microbalance measurements and from atomic force microscopy. The results from electrochemical impedance measurements and ellipsometry are consistent with these findings but these two methods are less able to distinguish between the formation of the monolayer and subsequent deposition of the bilayer on top of the monolayer. For DOPG films, where the head group is negatively charged at pH 8, the ionic strength of the solution has an important effect in controlling the deposition of the bilayer on top of the initially formed monolayer. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09599428_v10_n1_p149_Bartlett
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	phospholipid article crystal electrochemistry electrode film impedance ionic strength lipid analysis pH
spellingShingle	phospholipid article crystal electrochemistry electrode film impedance ionic strength lipid analysis pH Bartlett, P.N. Brace, K. Calvo, E.J. Etchenique, R. In situ characterization of phospholipid coated electrodes
topic_facet	phospholipid article crystal electrochemistry electrode film impedance ionic strength lipid analysis pH
description	Four in situ techniques, quartz crystal microbalance, electrochemical impedance spectroscopy, ellipsometry and atomic force microscopy, have been used to study the deposition of dioleoylphosphatidylglycerol (DOPG) films from vesicle solution onto 1-decanethiol coated gold surfaces. The experiments show that, although no one technique can give an unequivocal picture of the deposition process and structure of the final film, by combining the results from the four different techniques a consistent picture emerges. Initially, over the first 200 s a monolayer of DOPG is formed on the 1-decanethiol coated surface. Then at longer times and in the presence of 0.1 M NaCl and 0.1 M TRIS buffer at pH 8 a further bilayer of DOPG is deposited on top of this initially formed monolayer. Evidence for this is provided by the quartz crystal microbalance measurements and from atomic force microscopy. The results from electrochemical impedance measurements and ellipsometry are consistent with these findings but these two methods are less able to distinguish between the formation of the monolayer and subsequent deposition of the bilayer on top of the monolayer. For DOPG films, where the head group is negatively charged at pH 8, the ionic strength of the solution has an important effect in controlling the deposition of the bilayer on top of the initially formed monolayer.
format	JOUR
author	Bartlett, P.N. Brace, K. Calvo, E.J. Etchenique, R.
author_facet	Bartlett, P.N. Brace, K. Calvo, E.J. Etchenique, R.
author_sort	Bartlett, P.N.
title	In situ characterization of phospholipid coated electrodes
title_short	In situ characterization of phospholipid coated electrodes
title_full	In situ characterization of phospholipid coated electrodes
title_fullStr	In situ characterization of phospholipid coated electrodes
title_full_unstemmed	In situ characterization of phospholipid coated electrodes
title_sort	in situ characterization of phospholipid coated electrodes
url	http://hdl.handle.net/20.500.12110/paper_09599428_v10_n1_p149_Bartlett
work_keys_str_mv	AT bartlettpn insitucharacterizationofphospholipidcoatedelectrodes AT bracek insitucharacterizationofphospholipidcoatedelectrodes AT calvoej insitucharacterizationofphospholipidcoatedelectrodes AT etcheniquer insitucharacterizationofphospholipidcoatedelectrodes
_version_	1807321872507863040

In situ characterization of phospholipid coated electrodes

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