Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile

A comprehensive analysis of the carbohydrate-containing macromolecules from the coencocytic green seaweed Codium fragile and their arrangement in the cell wall was carried out. Cell walls in this seaweed are highly complex structures composed of 31% (w/w) of linear (1→4)-β-D-mannans, 9% (w/w) of pyr...

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Autores principales:	Estevez, J.M., Fernández, P.V., Kasulin, L., Dupree, P.
Formato:	JOUR
Materias:	β-mannan Codium cell wall Green seaweed HRGP epitope Pyruvylated arabinogalactan sulfate 3 linked beta levo arabinopyranose 3,5 linked alpha levo Araf 5 linked alpha levo Araf arabinogalactan sulfate epitope extensin hydroxyproline rich glycoprotein mannan oligosaccharide pyruvic acid terminal alpha levo Araf unclassified drug alga antibody labeling article carbohydrate analysis cell structure cell wall Codium fragile controlled study cytoarchitecture epitope mapping infrared spectroscopy macromolecule nonhuman priority journal protein localization residue analysis seaweed structure analysis synchrotron Cell Wall Epitopes Galactans Hydroxyproline Mannans Nuclear Magnetic Resonance, Biomolecular Seaweed Spectroscopy, Fourier Transform Infrared Sulfates Synchrotrons algae Ara Codium Tracheophyta
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09596658_v19_n3_p212_Estevez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_09596658_v19_n3_p212_Estevez2023-10-03T15:53:04Z Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile Estevez, J.M. Fernández, P.V. Kasulin, L. Dupree, P. Estevez, J.M. β-mannan Codium cell wall Green seaweed HRGP epitope Pyruvylated arabinogalactan sulfate 3 linked beta levo arabinopyranose 3,5 linked alpha levo Araf 5 linked alpha levo Araf arabinogalactan sulfate epitope extensin hydroxyproline rich glycoprotein mannan oligosaccharide pyruvic acid terminal alpha levo Araf unclassified drug alga antibody labeling article carbohydrate analysis cell structure cell wall Codium fragile controlled study cytoarchitecture epitope mapping infrared spectroscopy macromolecule nonhuman priority journal protein localization residue analysis seaweed structure analysis synchrotron Cell Wall Epitopes Galactans Hydroxyproline Mannans Nuclear Magnetic Resonance, Biomolecular Seaweed Spectroscopy, Fourier Transform Infrared Sulfates Synchrotrons algae Ara Codium Codium fragile Tracheophyta A comprehensive analysis of the carbohydrate-containing macromolecules from the coencocytic green seaweed Codium fragile and their arrangement in the cell wall was carried out. Cell walls in this seaweed are highly complex structures composed of 31% (w/w) of linear (1→4)-β-D-mannans, 9% (w/w) of pyruvylated arabinogalactan sulfates (pAGS), and low amounts of hydroxyproline rich-glycoprotein epitopes (HRGP). In situ chemical imaging by synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy and by immunolabeling using antibodies against specific cell wall carbohydrate epitopes revealed that β-D-mannans and pAGS are placed in the middle part of the cell wall, whereas HRGP epitopes (arabinogalactan proteins (AGPs) and extensins) are located on the wall boundaries, especially in the utricle apical zone. pAGS are sulfated at C-2 and/or C-4 of the 3-linked β-L-arabinopyranose units and at C-4 and/or C-6 of the 3-linked β-D-galactopyranose residues. In addition, high levels of ketals of pyruvic acid were found mainly at 3,4- of some terminal β-D-Gal p units forming a five-membered ring. Ramification was found at some C-6 of the 3-linked β-D-Gal p units. In agreement with the immunolabeled AGP epitopes, a nonsulfated branched furanosidic arabinan with 5-linked α-L-Ara f, 3,5-linked α-L-Ara f, and terminal α-L-Ara f units and a nonsulfated galactan structure composed of 3-(3,6)-linked β-D-Gal p residues, both typical of type-II AG glycans were found, suggesting that AGP structures are present at low levels in the cell walls of this seaweed. Based on this study, it is starting to emerge that Codium has developed unique cell wall architecture, when compared, not only with that of vascular plants, but also with other related green seaweeds and algae. © The Author 2008. Published by Oxford University Press. All rights reserved. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09596658_v19_n3_p212_Estevez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-mannan Codium cell wall Green seaweed HRGP epitope Pyruvylated arabinogalactan sulfate 3 linked beta levo arabinopyranose 3,5 linked alpha levo Araf 5 linked alpha levo Araf arabinogalactan sulfate epitope extensin hydroxyproline rich glycoprotein mannan oligosaccharide pyruvic acid terminal alpha levo Araf unclassified drug alga antibody labeling article carbohydrate analysis cell structure cell wall Codium fragile controlled study cytoarchitecture epitope mapping infrared spectroscopy macromolecule nonhuman priority journal protein localization residue analysis seaweed structure analysis synchrotron Cell Wall Epitopes Galactans Hydroxyproline Mannans Nuclear Magnetic Resonance, Biomolecular Seaweed Spectroscopy, Fourier Transform Infrared Sulfates Synchrotrons algae Ara Codium Codium fragile Tracheophyta
spellingShingle	β-mannan Codium cell wall Green seaweed HRGP epitope Pyruvylated arabinogalactan sulfate 3 linked beta levo arabinopyranose 3,5 linked alpha levo Araf 5 linked alpha levo Araf arabinogalactan sulfate epitope extensin hydroxyproline rich glycoprotein mannan oligosaccharide pyruvic acid terminal alpha levo Araf unclassified drug alga antibody labeling article carbohydrate analysis cell structure cell wall Codium fragile controlled study cytoarchitecture epitope mapping infrared spectroscopy macromolecule nonhuman priority journal protein localization residue analysis seaweed structure analysis synchrotron Cell Wall Epitopes Galactans Hydroxyproline Mannans Nuclear Magnetic Resonance, Biomolecular Seaweed Spectroscopy, Fourier Transform Infrared Sulfates Synchrotrons algae Ara Codium Codium fragile Tracheophyta Estevez, J.M. Fernández, P.V. Kasulin, L. Dupree, P. Estevez, J.M. Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
topic_facet	β-mannan Codium cell wall Green seaweed HRGP epitope Pyruvylated arabinogalactan sulfate 3 linked beta levo arabinopyranose 3,5 linked alpha levo Araf 5 linked alpha levo Araf arabinogalactan sulfate epitope extensin hydroxyproline rich glycoprotein mannan oligosaccharide pyruvic acid terminal alpha levo Araf unclassified drug alga antibody labeling article carbohydrate analysis cell structure cell wall Codium fragile controlled study cytoarchitecture epitope mapping infrared spectroscopy macromolecule nonhuman priority journal protein localization residue analysis seaweed structure analysis synchrotron Cell Wall Epitopes Galactans Hydroxyproline Mannans Nuclear Magnetic Resonance, Biomolecular Seaweed Spectroscopy, Fourier Transform Infrared Sulfates Synchrotrons algae Ara Codium Codium fragile Tracheophyta
description	A comprehensive analysis of the carbohydrate-containing macromolecules from the coencocytic green seaweed Codium fragile and their arrangement in the cell wall was carried out. Cell walls in this seaweed are highly complex structures composed of 31% (w/w) of linear (1→4)-β-D-mannans, 9% (w/w) of pyruvylated arabinogalactan sulfates (pAGS), and low amounts of hydroxyproline rich-glycoprotein epitopes (HRGP). In situ chemical imaging by synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy and by immunolabeling using antibodies against specific cell wall carbohydrate epitopes revealed that β-D-mannans and pAGS are placed in the middle part of the cell wall, whereas HRGP epitopes (arabinogalactan proteins (AGPs) and extensins) are located on the wall boundaries, especially in the utricle apical zone. pAGS are sulfated at C-2 and/or C-4 of the 3-linked β-L-arabinopyranose units and at C-4 and/or C-6 of the 3-linked β-D-galactopyranose residues. In addition, high levels of ketals of pyruvic acid were found mainly at 3,4- of some terminal β-D-Gal p units forming a five-membered ring. Ramification was found at some C-6 of the 3-linked β-D-Gal p units. In agreement with the immunolabeled AGP epitopes, a nonsulfated branched furanosidic arabinan with 5-linked α-L-Ara f, 3,5-linked α-L-Ara f, and terminal α-L-Ara f units and a nonsulfated galactan structure composed of 3-(3,6)-linked β-D-Gal p residues, both typical of type-II AG glycans were found, suggesting that AGP structures are present at low levels in the cell walls of this seaweed. Based on this study, it is starting to emerge that Codium has developed unique cell wall architecture, when compared, not only with that of vascular plants, but also with other related green seaweeds and algae. © The Author 2008. Published by Oxford University Press. All rights reserved.
format	JOUR
author	Estevez, J.M. Fernández, P.V. Kasulin, L. Dupree, P. Estevez, J.M.
author_facet	Estevez, J.M. Fernández, P.V. Kasulin, L. Dupree, P. Estevez, J.M.
author_sort	Estevez, J.M.
title	Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
title_short	Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
title_full	Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
title_fullStr	Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
title_full_unstemmed	Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
title_sort	chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed codium fragile
url	http://hdl.handle.net/20.500.12110/paper_09596658_v19_n3_p212_Estevez
work_keys_str_mv	AT estevezjm chemicalandinsitucharacterizationofmacromolecularcomponentsofthecellwallsfromthegreenseaweedcodiumfragile AT fernandezpv chemicalandinsitucharacterizationofmacromolecularcomponentsofthecellwallsfromthegreenseaweedcodiumfragile AT kasulinl chemicalandinsitucharacterizationofmacromolecularcomponentsofthecellwallsfromthegreenseaweedcodiumfragile AT dupreep chemicalandinsitucharacterizationofmacromolecularcomponentsofthecellwallsfromthegreenseaweedcodiumfragile AT estevezjm chemicalandinsitucharacterizationofmacromolecularcomponentsofthecellwallsfromthegreenseaweedcodiumfragile
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Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile

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