Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor
We cescribe the functional properties of a nicotinic α9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of α9 and channel properties of 5HT3A.Thus, it responded to acetylcholine in a concentration-dependent manner wi...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09594965_v14_n15_p1931_Verbitsky |
| Aporte de: |
| id |
todo:paper_09594965_v14_n15_p1931_Verbitsky |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09594965_v14_n15_p1931_Verbitsky2023-10-03T15:52:59Z Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor Verbitsky, M. Plazas, P.V. Elgoyhen, A.B. Hair cells Ion channels Nicotinic receptors Serotonin receptors acetylcholine atropine chimeric protein cholinergic receptor blocking agent glycine receptor antagonist methyllycaconitine muscarinic receptor blocking agent nicotine nicotinic receptor nicotinic receptor alpha5 nicotinic receptor alpha9 nicotinic receptor blocking agent receptor subtype receptor subunit serotonin serotonin 3 receptor serotonin 3a receptor strychnine unclassified drug aconitine Chrna9 protein, rat complementary DNA drug derivative hybrid protein methyllycaconitine nicotinic agent serotonin antagonist animal cell article concentration response drug potency drug receptor binding electric current electric potential female ligand binding nonhuman oocyte priority journal Xenopus laevis animal biosynthesis cell membrane potential drug effect genetics metabolism patch clamp physiology rat Aconitine Animals Atropine DNA, Complementary Membrane Potentials Muscarinic Antagonists Nicotine Nicotinic Agonists Nicotinic Antagonists Oocytes Patch-Clamp Techniques Rats Receptors, Nicotinic Receptors, Serotonin, 5-HT3 Recombinant Fusion Proteins Serotonin Antagonists Strychnine Xenopus laevis We cescribe the functional properties of a nicotinic α9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of α9 and channel properties of 5HT3A.Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 μM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as α9 receptors. The current-voltage relationship of currents through the α9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor. © 2003 Lippincott Williams & Wilkins. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09594965_v14_n15_p1931_Verbitsky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Hair cells Ion channels Nicotinic receptors Serotonin receptors acetylcholine atropine chimeric protein cholinergic receptor blocking agent glycine receptor antagonist methyllycaconitine muscarinic receptor blocking agent nicotine nicotinic receptor nicotinic receptor alpha5 nicotinic receptor alpha9 nicotinic receptor blocking agent receptor subtype receptor subunit serotonin serotonin 3 receptor serotonin 3a receptor strychnine unclassified drug aconitine Chrna9 protein, rat complementary DNA drug derivative hybrid protein methyllycaconitine nicotinic agent serotonin antagonist animal cell article concentration response drug potency drug receptor binding electric current electric potential female ligand binding nonhuman oocyte priority journal Xenopus laevis animal biosynthesis cell membrane potential drug effect genetics metabolism patch clamp physiology rat Aconitine Animals Atropine DNA, Complementary Membrane Potentials Muscarinic Antagonists Nicotine Nicotinic Agonists Nicotinic Antagonists Oocytes Patch-Clamp Techniques Rats Receptors, Nicotinic Receptors, Serotonin, 5-HT3 Recombinant Fusion Proteins Serotonin Antagonists Strychnine Xenopus laevis |
| spellingShingle |
Hair cells Ion channels Nicotinic receptors Serotonin receptors acetylcholine atropine chimeric protein cholinergic receptor blocking agent glycine receptor antagonist methyllycaconitine muscarinic receptor blocking agent nicotine nicotinic receptor nicotinic receptor alpha5 nicotinic receptor alpha9 nicotinic receptor blocking agent receptor subtype receptor subunit serotonin serotonin 3 receptor serotonin 3a receptor strychnine unclassified drug aconitine Chrna9 protein, rat complementary DNA drug derivative hybrid protein methyllycaconitine nicotinic agent serotonin antagonist animal cell article concentration response drug potency drug receptor binding electric current electric potential female ligand binding nonhuman oocyte priority journal Xenopus laevis animal biosynthesis cell membrane potential drug effect genetics metabolism patch clamp physiology rat Aconitine Animals Atropine DNA, Complementary Membrane Potentials Muscarinic Antagonists Nicotine Nicotinic Agonists Nicotinic Antagonists Oocytes Patch-Clamp Techniques Rats Receptors, Nicotinic Receptors, Serotonin, 5-HT3 Recombinant Fusion Proteins Serotonin Antagonists Strychnine Xenopus laevis Verbitsky, M. Plazas, P.V. Elgoyhen, A.B. Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| topic_facet |
Hair cells Ion channels Nicotinic receptors Serotonin receptors acetylcholine atropine chimeric protein cholinergic receptor blocking agent glycine receptor antagonist methyllycaconitine muscarinic receptor blocking agent nicotine nicotinic receptor nicotinic receptor alpha5 nicotinic receptor alpha9 nicotinic receptor blocking agent receptor subtype receptor subunit serotonin serotonin 3 receptor serotonin 3a receptor strychnine unclassified drug aconitine Chrna9 protein, rat complementary DNA drug derivative hybrid protein methyllycaconitine nicotinic agent serotonin antagonist animal cell article concentration response drug potency drug receptor binding electric current electric potential female ligand binding nonhuman oocyte priority journal Xenopus laevis animal biosynthesis cell membrane potential drug effect genetics metabolism patch clamp physiology rat Aconitine Animals Atropine DNA, Complementary Membrane Potentials Muscarinic Antagonists Nicotine Nicotinic Agonists Nicotinic Antagonists Oocytes Patch-Clamp Techniques Rats Receptors, Nicotinic Receptors, Serotonin, 5-HT3 Recombinant Fusion Proteins Serotonin Antagonists Strychnine Xenopus laevis |
| description |
We cescribe the functional properties of a nicotinic α9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of α9 and channel properties of 5HT3A.Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 μM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as α9 receptors. The current-voltage relationship of currents through the α9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor. © 2003 Lippincott Williams & Wilkins. |
| format |
JOUR |
| author |
Verbitsky, M. Plazas, P.V. Elgoyhen, A.B. |
| author_facet |
Verbitsky, M. Plazas, P.V. Elgoyhen, A.B. |
| author_sort |
Verbitsky, M. |
| title |
Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| title_short |
Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| title_full |
Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| title_fullStr |
Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| title_full_unstemmed |
Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor |
| title_sort |
functional expression and properties of a nicotinic α9/5-ht3 a chimeric receptor |
| url |
http://hdl.handle.net/20.500.12110/paper_09594965_v14_n15_p1931_Verbitsky |
| work_keys_str_mv |
AT verbitskym functionalexpressionandpropertiesofanicotinica95ht3achimericreceptor AT plazaspv functionalexpressionandpropertiesofanicotinica95ht3achimericreceptor AT elgoyhenab functionalexpressionandpropertiesofanicotinica95ht3achimericreceptor |
| _version_ |
1782023600677584896 |