Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations

The effectiveness of hydrogen peroxide on the destruction of planktonic cells of Escherichia coli at different temperatures, pH and sanitizer concentrations was studied. Inactivation kinetics of E. coli exhibited a clear dependence on hydrogen peroxide concentration, pH and temperature. A Weibullian...

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Detalles Bibliográficos
Autores principales: Raffellini, S., Schenk, M., Guerrero, S., Alzamora, S.M.
Formato: JOUR
Materias:
E. coli
Hydrogen peroxide
Inactivation kinetics
PH
Temperature
Time to 5-log reductions
Escherichia coli
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09567135_v22_n6_p920_Raffellini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09567135_v22_n6_p920_Raffellini
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spelling todo:paper_09567135_v22_n6_p920_Raffellini2023-10-03T15:52:12Z Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations Raffellini, S. Schenk, M. Guerrero, S. Alzamora, S.M. E. coli Hydrogen peroxide Inactivation kinetics PH Temperature Time to 5-log reductions Escherichia coli The effectiveness of hydrogen peroxide on the destruction of planktonic cells of Escherichia coli at different temperatures, pH and sanitizer concentrations was studied. Inactivation kinetics of E. coli exhibited a clear dependence on hydrogen peroxide concentration, pH and temperature. A Weibullian mathematical model successfully described the inactivation curves. Quantitative kinetic results obtained allowed to identify various combinations H2O2 concentration-pH-temperature for 5-log cycles reduction of E. coli. Flow cytometry analysis revealed induced H2O2 cytoplasm membrane damage. TEM observations indicated that H2O2 treatment resulted in rupture of outer and cytoplasm membranes and a more uniform granularity. © 2010 Elsevier Ltd. Fil:Raffellini, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schenk, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerrero, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alzamora, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09567135_v22_n6_p920_Raffellini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic E. coli
Hydrogen peroxide
Inactivation kinetics
PH
Temperature
Time to 5-log reductions
Escherichia coli
spellingShingle E. coli
Hydrogen peroxide
Inactivation kinetics
PH
Temperature
Time to 5-log reductions
Escherichia coli
Raffellini, S.
Schenk, M.
Guerrero, S.
Alzamora, S.M.
Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
topic_facet E. coli
Hydrogen peroxide
Inactivation kinetics
PH
Temperature
Time to 5-log reductions
Escherichia coli
description The effectiveness of hydrogen peroxide on the destruction of planktonic cells of Escherichia coli at different temperatures, pH and sanitizer concentrations was studied. Inactivation kinetics of E. coli exhibited a clear dependence on hydrogen peroxide concentration, pH and temperature. A Weibullian mathematical model successfully described the inactivation curves. Quantitative kinetic results obtained allowed to identify various combinations H2O2 concentration-pH-temperature for 5-log cycles reduction of E. coli. Flow cytometry analysis revealed induced H2O2 cytoplasm membrane damage. TEM observations indicated that H2O2 treatment resulted in rupture of outer and cytoplasm membranes and a more uniform granularity. © 2010 Elsevier Ltd.
format JOUR
author Raffellini, S.
Schenk, M.
Guerrero, S.
Alzamora, S.M.
author_facet Raffellini, S.
Schenk, M.
Guerrero, S.
Alzamora, S.M.
author_sort Raffellini, S.
title Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
title_short Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
title_full Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
title_fullStr Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
title_full_unstemmed Kinetics of Escherichia coli inactivation employing hydrogen peroxide at varying temperatures, pH and concentrations
title_sort kinetics of escherichia coli inactivation employing hydrogen peroxide at varying temperatures, ph and concentrations
url http://hdl.handle.net/20.500.12110/paper_09567135_v22_n6_p920_Raffellini
work_keys_str_mv AT raffellinis kineticsofescherichiacoliinactivationemployinghydrogenperoxideatvaryingtemperaturesphandconcentrations
AT schenkm kineticsofescherichiacoliinactivationemployinghydrogenperoxideatvaryingtemperaturesphandconcentrations
AT guerreros kineticsofescherichiacoliinactivationemployinghydrogenperoxideatvaryingtemperaturesphandconcentrations
AT alzamorasm kineticsofescherichiacoliinactivationemployinghydrogenperoxideatvaryingtemperaturesphandconcentrations
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