Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan

The resistance of Saccharomyces cerevisiae to the action of ultrasound (600 W, 20 kHz, 95.2 μm wave amplitude) at 45 °C in Sabouraud broth (pH 5.6) containing 1000 ppm low weight chitosan was analyzed. Preincubation of the inoculated systems 30 and 90 min prior to sonication was also evaluated. Addi...

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Detalles Bibliográficos
Autores principales: Guerrero, S., Tognon, M., Alzamora, S.M.
Formato: JOUR
Materias:
Chitosan
Gompertz equation
Inactivation
S. cerevisiae
Ultrasound
Ultrastructural changes
Weibull distribution
chitosan
amplitude modulation
article
calculation
cell survival
controlled study
food preservation
frequency analysis
incubation time
molecular weight
nonhuman
pH
Saccharomyces cerevisiae
temperature measurement
transmission electron microscopy
ultrasound
Saccharomyces
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09567135_v16_n2_p131_Guerrero
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09567135_v16_n2_p131_Guerrero
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spelling todo:paper_09567135_v16_n2_p131_Guerrero2023-10-03T15:52:10Z Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan Guerrero, S. Tognon, M. Alzamora, S.M. Chitosan Gompertz equation Inactivation S. cerevisiae Ultrasound Ultrastructural changes Weibull distribution chitosan amplitude modulation article calculation cell survival controlled study food preservation frequency analysis incubation time molecular weight nonhuman pH Saccharomyces cerevisiae temperature measurement transmission electron microscopy ultrasound Saccharomyces Saccharomyces cerevisiae The resistance of Saccharomyces cerevisiae to the action of ultrasound (600 W, 20 kHz, 95.2 μm wave amplitude) at 45 °C in Sabouraud broth (pH 5.6) containing 1000 ppm low weight chitosan was analyzed. Preincubation of the inoculated systems 30 and 90 min prior to sonication was also evaluated. Addition of chitosan enhanced the inactivation by ultrasound. Incubating the yeast with chitosan prior to sonication increased the inactivation. After 30 min of exposure to chitosan, approximately 1-log cycle reduction of the yeast was obtained leading to a final reduction of more than three log cycles after 30 min of the ultrasonic treatment. Ninety minutes of exposure to chitosan resulted in little significant change in yeast reduction. Transmission electron microphotographs of the cells showed possible different action mechanisms of chitosan depending on whether systems were preincubated or not. Experimental data were modeled by using the modified Gompertz equation and Weibull distribution of resistances. © 2004 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09567135_v16_n2_p131_Guerrero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chitosan
Gompertz equation
Inactivation
S. cerevisiae
Ultrasound
Ultrastructural changes
Weibull distribution
chitosan
amplitude modulation
article
calculation
cell survival
controlled study
food preservation
frequency analysis
incubation time
molecular weight
nonhuman
pH
Saccharomyces cerevisiae
temperature measurement
transmission electron microscopy
ultrasound
Saccharomyces
Saccharomyces cerevisiae
spellingShingle Chitosan
Gompertz equation
Inactivation
S. cerevisiae
Ultrasound
Ultrastructural changes
Weibull distribution
chitosan
amplitude modulation
article
calculation
cell survival
controlled study
food preservation
frequency analysis
incubation time
molecular weight
nonhuman
pH
Saccharomyces cerevisiae
temperature measurement
transmission electron microscopy
ultrasound
Saccharomyces
Saccharomyces cerevisiae
Guerrero, S.
Tognon, M.
Alzamora, S.M.
Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
topic_facet Chitosan
Gompertz equation
Inactivation
S. cerevisiae
Ultrasound
Ultrastructural changes
Weibull distribution
chitosan
amplitude modulation
article
calculation
cell survival
controlled study
food preservation
frequency analysis
incubation time
molecular weight
nonhuman
pH
Saccharomyces cerevisiae
temperature measurement
transmission electron microscopy
ultrasound
Saccharomyces
Saccharomyces cerevisiae
description The resistance of Saccharomyces cerevisiae to the action of ultrasound (600 W, 20 kHz, 95.2 μm wave amplitude) at 45 °C in Sabouraud broth (pH 5.6) containing 1000 ppm low weight chitosan was analyzed. Preincubation of the inoculated systems 30 and 90 min prior to sonication was also evaluated. Addition of chitosan enhanced the inactivation by ultrasound. Incubating the yeast with chitosan prior to sonication increased the inactivation. After 30 min of exposure to chitosan, approximately 1-log cycle reduction of the yeast was obtained leading to a final reduction of more than three log cycles after 30 min of the ultrasonic treatment. Ninety minutes of exposure to chitosan resulted in little significant change in yeast reduction. Transmission electron microphotographs of the cells showed possible different action mechanisms of chitosan depending on whether systems were preincubated or not. Experimental data were modeled by using the modified Gompertz equation and Weibull distribution of resistances. © 2004 Elsevier Ltd. All rights reserved.
format JOUR
author Guerrero, S.
Tognon, M.
Alzamora, S.M.
author_facet Guerrero, S.
Tognon, M.
Alzamora, S.M.
author_sort Guerrero, S.
title Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
title_short Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
title_full Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
title_fullStr Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
title_full_unstemmed Response of Saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
title_sort response of saccharomyces cerevisiae to the combined action of ultrasound and low weight chitosan
url http://hdl.handle.net/20.500.12110/paper_09567135_v16_n2_p131_Guerrero
work_keys_str_mv AT guerreros responseofsaccharomycescerevisiaetothecombinedactionofultrasoundandlowweightchitosan
AT tognonm responseofsaccharomycescerevisiaetothecombinedactionofultrasoundandlowweightchitosan
AT alzamorasm responseofsaccharomycescerevisiaetothecombinedactionofultrasoundandlowweightchitosan
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