Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina
The Rhizophydiales is the most recently circumscribed order in the Chytridiomycota. Past studies focused on soil chytrids from North America and Australia to determine the range of diversity within this clade of chytrids and established three families (Rhizophydiaceae, Terramycetaceae, and Kappamyce...
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todo:paper_09537562_v112_n7_p759_Letcher2023-10-03T15:51:13Z Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina Letcher, P.M. Vélez, C.G. Barrantes, M.E. Powell, M.J. Churchill, P.F. Wakefield, W.S. Chytrid Phylogeny Ribosomal genes Taxonomy Zoospore fungal DNA ribosomal spacer DNA ribosome DNA RNA 28S fungus genetic variation molecular analysis morphology new genus phylogeny spore taxonomy ultrastructure Argentina article Chytridiomycetes classification genetics geography isolation and purification molecular genetics North America phylogeny transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal DNA, Ribosomal DNA, Ribosomal Spacer Geography Microscopy, Electron, Transmission Molecular Sequence Data North America Phylogeny RNA, Ribosomal, 28S Argentina North America South America Chytridiomycota The Rhizophydiales is the most recently circumscribed order in the Chytridiomycota. Past studies focused on soil chytrids from North America and Australia to determine the range of diversity within this clade of chytrids and established three families (Rhizophydiaceae, Terramycetaceae, and Kappamycetaceae) in the new order. Although Rhizophydiales contains seemingly simple chytrids morphologically, analyses of ribosomal gene sequences and zoospore characters have demonstrated unexpected genetic and ultrastructural diversity, highlighting the need for broader habitat and geographic sampling to reveal the actual diversity within this new order. To enlarge our sampling, in this study we investigated 38 newly cultured chytrids collected from aquatic habitats in Argentina, a territory under-explored for chytrid diversity. From analyses of thallus morphology, zoospore ultrastructure, and 28S and ITS1-5.8S-ITS2 ribosomal gene sequences, we expand the concept of Rhizophydiales, describing seven new families (Alphamycetaceae, Angulomycetaceae, Aquamycetaceae, Globomycetaceae, Gorgonomycetaceae, Pateramycetaceae, and Protrudomycetaceae) and eight new genera (Alphamyces, Angulomyces, Aquamyces, Globomyces, Urceomyces, Gorgonomyces, Pateramyces, and Protrudomyces). Results of this study underscore that even more extensive sampling from varied habitats and geographies is needed to adequately ascertain the diversity of chytrids in the Rhizophydiales. © 2008 The British Mycological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09537562_v112_n7_p759_Letcher |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chytrid Phylogeny Ribosomal genes Taxonomy Zoospore fungal DNA ribosomal spacer DNA ribosome DNA RNA 28S fungus genetic variation molecular analysis morphology new genus phylogeny spore taxonomy ultrastructure Argentina article Chytridiomycetes classification genetics geography isolation and purification molecular genetics North America phylogeny transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal DNA, Ribosomal DNA, Ribosomal Spacer Geography Microscopy, Electron, Transmission Molecular Sequence Data North America Phylogeny RNA, Ribosomal, 28S Argentina North America South America Chytridiomycota |
| spellingShingle |
Chytrid Phylogeny Ribosomal genes Taxonomy Zoospore fungal DNA ribosomal spacer DNA ribosome DNA RNA 28S fungus genetic variation molecular analysis morphology new genus phylogeny spore taxonomy ultrastructure Argentina article Chytridiomycetes classification genetics geography isolation and purification molecular genetics North America phylogeny transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal DNA, Ribosomal DNA, Ribosomal Spacer Geography Microscopy, Electron, Transmission Molecular Sequence Data North America Phylogeny RNA, Ribosomal, 28S Argentina North America South America Chytridiomycota Letcher, P.M. Vélez, C.G. Barrantes, M.E. Powell, M.J. Churchill, P.F. Wakefield, W.S. Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| topic_facet |
Chytrid Phylogeny Ribosomal genes Taxonomy Zoospore fungal DNA ribosomal spacer DNA ribosome DNA RNA 28S fungus genetic variation molecular analysis morphology new genus phylogeny spore taxonomy ultrastructure Argentina article Chytridiomycetes classification genetics geography isolation and purification molecular genetics North America phylogeny transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal DNA, Ribosomal DNA, Ribosomal Spacer Geography Microscopy, Electron, Transmission Molecular Sequence Data North America Phylogeny RNA, Ribosomal, 28S Argentina North America South America Chytridiomycota |
| description |
The Rhizophydiales is the most recently circumscribed order in the Chytridiomycota. Past studies focused on soil chytrids from North America and Australia to determine the range of diversity within this clade of chytrids and established three families (Rhizophydiaceae, Terramycetaceae, and Kappamycetaceae) in the new order. Although Rhizophydiales contains seemingly simple chytrids morphologically, analyses of ribosomal gene sequences and zoospore characters have demonstrated unexpected genetic and ultrastructural diversity, highlighting the need for broader habitat and geographic sampling to reveal the actual diversity within this new order. To enlarge our sampling, in this study we investigated 38 newly cultured chytrids collected from aquatic habitats in Argentina, a territory under-explored for chytrid diversity. From analyses of thallus morphology, zoospore ultrastructure, and 28S and ITS1-5.8S-ITS2 ribosomal gene sequences, we expand the concept of Rhizophydiales, describing seven new families (Alphamycetaceae, Angulomycetaceae, Aquamycetaceae, Globomycetaceae, Gorgonomycetaceae, Pateramycetaceae, and Protrudomycetaceae) and eight new genera (Alphamyces, Angulomyces, Aquamyces, Globomyces, Urceomyces, Gorgonomyces, Pateramyces, and Protrudomyces). Results of this study underscore that even more extensive sampling from varied habitats and geographies is needed to adequately ascertain the diversity of chytrids in the Rhizophydiales. © 2008 The British Mycological Society. |
| format |
JOUR |
| author |
Letcher, P.M. Vélez, C.G. Barrantes, M.E. Powell, M.J. Churchill, P.F. Wakefield, W.S. |
| author_facet |
Letcher, P.M. Vélez, C.G. Barrantes, M.E. Powell, M.J. Churchill, P.F. Wakefield, W.S. |
| author_sort |
Letcher, P.M. |
| title |
Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| title_short |
Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| title_full |
Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| title_fullStr |
Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| title_full_unstemmed |
Ultrastructural and molecular analyses of Rhizophydiales (Chytridiomycota) isolates from North America and Argentina |
| title_sort |
ultrastructural and molecular analyses of rhizophydiales (chytridiomycota) isolates from north america and argentina |
| url |
http://hdl.handle.net/20.500.12110/paper_09537562_v112_n7_p759_Letcher |
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