The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin
The effects of leukotriene C4 (LTC4), nordihydroguaiaretic acid (NDGA) and FPL-55712, on the metabolism of labelled glucose (U14C-glucose) in uteri isolated from spayed rats and from spayed-estrogenized rats, incubated in the presence and in the absence of indomethacin, were explored. Indomethacin (...
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todo:paper_09523278_v36_n2_p113_Gonzalez2023-10-03T15:50:44Z The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin Gonzalez, E.T. Gimeno, A.L. Gimeno, M.A.F. 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid carbon dioxide chromone derivative estradiol glucose indometacin nordihydroguaiaretic acid slow reacting substance 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid estradiol glucose indometacin leukotriene c4 nordihydroguaiaretic acid radioisotope animal article enzymology female in vitro study metabolism ovariectomy rat rat strain uterus animal cell metabolism nonhuman priority journal subcutaneous drug administration Animal Carbon Dioxide Chromones Estradiol Female Glucose In Vitro Indomethacin Nordihydroguaiaretic Acid Ovariectomy Rats Rats, Inbred Strains SRS-A Support, Non-U.S. Gov't Uterus The effects of leukotriene C4 (LTC4), nordihydroguaiaretic acid (NDGA) and FPL-55712, on the metabolism of labelled glucose (U14C-glucose) in uteri isolated from spayed rats and from spayed-estrogenized rats, incubated in the presence and in the absence of indomethacin, were explored. Indomethacin (10-6M), enhanced significantly 14CO2 formation from labelled glucose, both in uteri from ovariectomized rats and in uteri from ovariectomized-estrogenized animals. In uteri from spayed not-estrogenized rats, exposed 'in vitro' to indomethacin, NDGA (10-5M), an inhibitor of the 5-lipoxygenase, as well as FPL-55712 (10-5M), a LT antagonist, reduced significantly the enhanced metabolism of glucose evoked by indomethacin, an inhibitor of the cyclo- oxygenase. On the other hand, LTC4 (10-7M) augmented the metabolism of labelled glucose, reaching values even greater than those induced by indomethacin. In the spayed-estrogenized group LTC4 (10-10 - 10-7M) enhanced the formation of labelled CO2 from labelled glucose as much as indomethacin (10-6M) did, whereas neither NDGA nor FPL-55712 were effective. In addition, in uteri from ovariectomized-estrogenized rats, incubated with indomethacin, NDGA and FPL-55712, decreased the augmenting action of indomethacin on glucose metabolism, whereas LTC4 (10-10 - 10-7M) evoked a complete reversal of the inhibitory influence of NDGA on the formation of 14CO2. The force-going results suggest that tissue 5-lipoxygenase products, particularly LTC4, are involved in the metabolism of labelled glucose by rat uteri, mainly when the cyclo-oxygenase pathway is inhibited by indomethacin and the tissue is deprived of estradiol. Moreover, under estrogenic dominance, exogenous LTC4 exhibits a peculiar influence manifested by an enhancement of the uterine metabolism of U14C-glucose. © 1989. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09523278_v36_n2_p113_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid carbon dioxide chromone derivative estradiol glucose indometacin nordihydroguaiaretic acid slow reacting substance 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid estradiol glucose indometacin leukotriene c4 nordihydroguaiaretic acid radioisotope animal article enzymology female in vitro study metabolism ovariectomy rat rat strain uterus animal cell metabolism nonhuman priority journal subcutaneous drug administration Animal Carbon Dioxide Chromones Estradiol Female Glucose In Vitro Indomethacin Nordihydroguaiaretic Acid Ovariectomy Rats Rats, Inbred Strains SRS-A Support, Non-U.S. Gov't Uterus |
| spellingShingle |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid carbon dioxide chromone derivative estradiol glucose indometacin nordihydroguaiaretic acid slow reacting substance 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid estradiol glucose indometacin leukotriene c4 nordihydroguaiaretic acid radioisotope animal article enzymology female in vitro study metabolism ovariectomy rat rat strain uterus animal cell metabolism nonhuman priority journal subcutaneous drug administration Animal Carbon Dioxide Chromones Estradiol Female Glucose In Vitro Indomethacin Nordihydroguaiaretic Acid Ovariectomy Rats Rats, Inbred Strains SRS-A Support, Non-U.S. Gov't Uterus Gonzalez, E.T. Gimeno, A.L. Gimeno, M.A.F. The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| topic_facet |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid carbon dioxide chromone derivative estradiol glucose indometacin nordihydroguaiaretic acid slow reacting substance 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid estradiol glucose indometacin leukotriene c4 nordihydroguaiaretic acid radioisotope animal article enzymology female in vitro study metabolism ovariectomy rat rat strain uterus animal cell metabolism nonhuman priority journal subcutaneous drug administration Animal Carbon Dioxide Chromones Estradiol Female Glucose In Vitro Indomethacin Nordihydroguaiaretic Acid Ovariectomy Rats Rats, Inbred Strains SRS-A Support, Non-U.S. Gov't Uterus |
| description |
The effects of leukotriene C4 (LTC4), nordihydroguaiaretic acid (NDGA) and FPL-55712, on the metabolism of labelled glucose (U14C-glucose) in uteri isolated from spayed rats and from spayed-estrogenized rats, incubated in the presence and in the absence of indomethacin, were explored. Indomethacin (10-6M), enhanced significantly 14CO2 formation from labelled glucose, both in uteri from ovariectomized rats and in uteri from ovariectomized-estrogenized animals. In uteri from spayed not-estrogenized rats, exposed 'in vitro' to indomethacin, NDGA (10-5M), an inhibitor of the 5-lipoxygenase, as well as FPL-55712 (10-5M), a LT antagonist, reduced significantly the enhanced metabolism of glucose evoked by indomethacin, an inhibitor of the cyclo- oxygenase. On the other hand, LTC4 (10-7M) augmented the metabolism of labelled glucose, reaching values even greater than those induced by indomethacin. In the spayed-estrogenized group LTC4 (10-10 - 10-7M) enhanced the formation of labelled CO2 from labelled glucose as much as indomethacin (10-6M) did, whereas neither NDGA nor FPL-55712 were effective. In addition, in uteri from ovariectomized-estrogenized rats, incubated with indomethacin, NDGA and FPL-55712, decreased the augmenting action of indomethacin on glucose metabolism, whereas LTC4 (10-10 - 10-7M) evoked a complete reversal of the inhibitory influence of NDGA on the formation of 14CO2. The force-going results suggest that tissue 5-lipoxygenase products, particularly LTC4, are involved in the metabolism of labelled glucose by rat uteri, mainly when the cyclo-oxygenase pathway is inhibited by indomethacin and the tissue is deprived of estradiol. Moreover, under estrogenic dominance, exogenous LTC4 exhibits a peculiar influence manifested by an enhancement of the uterine metabolism of U14C-glucose. © 1989. |
| format |
JOUR |
| author |
Gonzalez, E.T. Gimeno, A.L. Gimeno, M.A.F. |
| author_facet |
Gonzalez, E.T. Gimeno, A.L. Gimeno, M.A.F. |
| author_sort |
Gonzalez, E.T. |
| title |
The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| title_short |
The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| title_full |
The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| title_fullStr |
The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| title_full_unstemmed |
The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
| title_sort |
influences of leukotriene c4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. effects of indomethacin |
| url |
http://hdl.handle.net/20.500.12110/paper_09523278_v36_n2_p113_Gonzalez |
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