Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices

The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental vari...

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Autores principales: Monge, M.E., Negri, R.M., Kolender, A.A., Erra-Balsells, R.
Formato: JOUR
Materias:
2,5-dihydroxybenzoic acid
drug derivative
gentisic acid
harman
harmine
pectin
unclassified drug
article
chemistry
electrospray mass spectrometry
hydroxylation
mass spectrometry
methodology
nuclear magnetic resonance spectroscopy
reproducibility
sensitivity and specificity
ultraviolet spectrophotometry
Gentisates
Harmine
Hydroxylation
Magnetic Resonance Spectroscopy
Pectins
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09514198_v21_n16_p2638_Monge2023-10-03T15:50:41Z Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices Monge, M.E. Negri, R.M. Kolender, A.A. Erra-Balsells, R. 2,5-dihydroxybenzoic acid drug derivative gentisic acid harman harmine pectin unclassified drug article chemistry electrospray mass spectrometry hydroxylation mass spectrometry methodology nuclear magnetic resonance spectroscopy reproducibility sensitivity and specificity ultraviolet spectrophotometry Gentisates Harmine Hydroxylation Magnetic Resonance Spectroscopy Pectins Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOF MS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. Copyright © 2007 John Wiley & Sons, Ltd. Fil:Monge, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Negri, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kolender, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 2,5-dihydroxybenzoic acid
drug derivative
gentisic acid
harman
harmine
pectin
unclassified drug
article
chemistry
electrospray mass spectrometry
hydroxylation
mass spectrometry
methodology
nuclear magnetic resonance spectroscopy
reproducibility
sensitivity and specificity
ultraviolet spectrophotometry
Gentisates
Harmine
Hydroxylation
Magnetic Resonance Spectroscopy
Pectins
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
spellingShingle 2,5-dihydroxybenzoic acid
drug derivative
gentisic acid
harman
harmine
pectin
unclassified drug
article
chemistry
electrospray mass spectrometry
hydroxylation
mass spectrometry
methodology
nuclear magnetic resonance spectroscopy
reproducibility
sensitivity and specificity
ultraviolet spectrophotometry
Gentisates
Harmine
Hydroxylation
Magnetic Resonance Spectroscopy
Pectins
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Monge, M.E.
Negri, R.M.
Kolender, A.A.
Erra-Balsells, R.
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
topic_facet 2,5-dihydroxybenzoic acid
drug derivative
gentisic acid
harman
harmine
pectin
unclassified drug
article
chemistry
electrospray mass spectrometry
hydroxylation
mass spectrometry
methodology
nuclear magnetic resonance spectroscopy
reproducibility
sensitivity and specificity
ultraviolet spectrophotometry
Gentisates
Harmine
Hydroxylation
Magnetic Resonance Spectroscopy
Pectins
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
description The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOF MS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. Copyright © 2007 John Wiley & Sons, Ltd.
format JOUR
author Monge, M.E.
Negri, R.M.
Kolender, A.A.
Erra-Balsells, R.
author_facet Monge, M.E.
Negri, R.M.
Kolender, A.A.
Erra-Balsells, R.
author_sort Monge, M.E.
title Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
title_short Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
title_full Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
title_fullStr Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
title_full_unstemmed Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
title_sort structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as uv-maldi matrices
url http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge
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AT negrirm structuralcharacterizationofnativehighmethoxylatedpectinusingnuclearmagneticresonancespectroscopyandultravioletmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrycomparativeuseof25dihydroxybenzoicacidandnorharmaneasuvmaldimatrices
AT kolenderaa structuralcharacterizationofnativehighmethoxylatedpectinusingnuclearmagneticresonancespectroscopyandultravioletmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrycomparativeuseof25dihydroxybenzoicacidandnorharmaneasuvmaldimatrices
AT errabalsellsr structuralcharacterizationofnativehighmethoxylatedpectinusingnuclearmagneticresonancespectroscopyandultravioletmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrycomparativeuseof25dihydroxybenzoicacidandnorharmaneasuvmaldimatrices
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