A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to elec...
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| Formato: | JOUR |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman |
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todo:paper_0951208X_v1_n3_p163_Schijman2023-10-03T15:50:33Z A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. Schijman, A.G. Floccari, M.E. Méndez, B.S. A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Floccari, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall. |
| format |
JOUR |
| author |
Schijman, A.G. Floccari, M.E. Méndez, B.S. |
| spellingShingle |
Schijman, A.G. Floccari, M.E. Méndez, B.S. A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| author_facet |
Schijman, A.G. Floccari, M.E. Méndez, B.S. |
| author_sort |
Schijman, A.G. |
| title |
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| title_short |
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| title_full |
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| title_fullStr |
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| title_full_unstemmed |
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| title_sort |
rapid method for the screening of plasmids from clostridium acetobutylicum and other clostridium sp. |
| url |
http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman |
| work_keys_str_mv |
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1807322595654107136 |