Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison

The composition of planktonic eukaryotes in the size fraction 3-20 μm of 10 maritime Antarctic lakes was studied by denaturing gradient gel electrophoresis (DGGE). Microscopic observations were also carried out to compare the results obtained by this molecular fingerprinting technique with morpholog...

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Autores principales: Unrein, F., Izaguirre, I., Massana, R., Balagué, V., Gasol, J.M.
Formato: JOUR
Materias:
18S rDNA
Antarctic lakes
DGGE
Freshwater nanoplankton
Bacillariophyceae
Cercozoa
Chlorophyceae
Chrysophyceae
Chrysosphaerales
Dictyochophyceae
Eukaryota
Ochromonadales
Pedinellales
Pseudopedinella
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09483055_v40_n3_p269_Unrein
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09483055_v40_n3_p269_Unrein
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spelling todo:paper_09483055_v40_n3_p269_Unrein2023-10-03T15:49:33Z Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison Unrein, F. Izaguirre, I. Massana, R. Balagué, V. Gasol, J.M. 18S rDNA Antarctic lakes DGGE Freshwater nanoplankton Bacillariophyceae Cercozoa Chlorophyceae Chrysophyceae Chrysosphaerales Dictyochophyceae Eukaryota Ochromonadales Pedinellales Pseudopedinella The composition of planktonic eukaryotes in the size fraction 3-20 μm of 10 maritime Antarctic lakes was studied by denaturing gradient gel electrophoresis (DGGE). Microscopic observations were also carried out to compare the results obtained by this molecular fingerprinting technique with morphological data. Six lakes from Hope Bay (Antarctic Peninsula) and 4 from the Potter Peninsula (King George Island) were sampled during the austral summer of 2003. These lakes were of different trophic status and covered a wide range of limnological features. Previous studies of the planktonic communities of these lakes revealed high nanoflagellate abundance and biomass, but their taxonomic identification was usually uncertain due to their similarity in size and shape. Here, the application of DGGE allowed both a comparison of the structure of the nanoplanktonic communities and an identification of the dominant populations through sequencing of the most prominent DGGE bands. The most important organisms in these lakes were the Chrysophyceae, represented in the DGGE gel by 5 different band positions and identified by microscopy in 5 different morphotypes, including uniflagellated and biflagellated naked organisms: 1 sequence belonged to the Chrysosphaerales, 2 closely related bands (likely 2 species from the same genus) belonged probably to the Ochromonadales (unicellular biflagellates), while the other 2 bands could not be assigned to any defined chrysophyte group. Sequences related to Chlorophyceae, Bacillariophyceae and probably Cercozoa were also retrieved. A Dictyochophyceae belonging to the order Pedinellales is reported for the first time in freshwater Antarctic ecosystems. Microscopic observations suggest that this phytoplanktonic organism most likely corresponds to Pseudopedinella. Most of the lakes shared several common sequences, such as 2 chrysophyte bands, which suggests the existence of well-adapted nanoplanktonic species dispersed throughout the Antarctic lakes. However, some sequences appeared exclusively in specific lakes, which seems to be related to the trophic status of the water bodies and probably also to the local conditions of the maritime Antarctic regions sampled. © Inter-Research 2005. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09483055_v40_n3_p269_Unrein
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 18S rDNA
Antarctic lakes
DGGE
Freshwater nanoplankton
Bacillariophyceae
Cercozoa
Chlorophyceae
Chrysophyceae
Chrysosphaerales
Dictyochophyceae
Eukaryota
Ochromonadales
Pedinellales
Pseudopedinella
spellingShingle 18S rDNA
Antarctic lakes
DGGE
Freshwater nanoplankton
Bacillariophyceae
Cercozoa
Chlorophyceae
Chrysophyceae
Chrysosphaerales
Dictyochophyceae
Eukaryota
Ochromonadales
Pedinellales
Pseudopedinella
Unrein, F.
Izaguirre, I.
Massana, R.
Balagué, V.
Gasol, J.M.
Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
topic_facet 18S rDNA
Antarctic lakes
DGGE
Freshwater nanoplankton
Bacillariophyceae
Cercozoa
Chlorophyceae
Chrysophyceae
Chrysosphaerales
Dictyochophyceae
Eukaryota
Ochromonadales
Pedinellales
Pseudopedinella
description The composition of planktonic eukaryotes in the size fraction 3-20 μm of 10 maritime Antarctic lakes was studied by denaturing gradient gel electrophoresis (DGGE). Microscopic observations were also carried out to compare the results obtained by this molecular fingerprinting technique with morphological data. Six lakes from Hope Bay (Antarctic Peninsula) and 4 from the Potter Peninsula (King George Island) were sampled during the austral summer of 2003. These lakes were of different trophic status and covered a wide range of limnological features. Previous studies of the planktonic communities of these lakes revealed high nanoflagellate abundance and biomass, but their taxonomic identification was usually uncertain due to their similarity in size and shape. Here, the application of DGGE allowed both a comparison of the structure of the nanoplanktonic communities and an identification of the dominant populations through sequencing of the most prominent DGGE bands. The most important organisms in these lakes were the Chrysophyceae, represented in the DGGE gel by 5 different band positions and identified by microscopy in 5 different morphotypes, including uniflagellated and biflagellated naked organisms: 1 sequence belonged to the Chrysosphaerales, 2 closely related bands (likely 2 species from the same genus) belonged probably to the Ochromonadales (unicellular biflagellates), while the other 2 bands could not be assigned to any defined chrysophyte group. Sequences related to Chlorophyceae, Bacillariophyceae and probably Cercozoa were also retrieved. A Dictyochophyceae belonging to the order Pedinellales is reported for the first time in freshwater Antarctic ecosystems. Microscopic observations suggest that this phytoplanktonic organism most likely corresponds to Pseudopedinella. Most of the lakes shared several common sequences, such as 2 chrysophyte bands, which suggests the existence of well-adapted nanoplanktonic species dispersed throughout the Antarctic lakes. However, some sequences appeared exclusively in specific lakes, which seems to be related to the trophic status of the water bodies and probably also to the local conditions of the maritime Antarctic regions sampled. © Inter-Research 2005.
format JOUR
author Unrein, F.
Izaguirre, I.
Massana, R.
Balagué, V.
Gasol, J.M.
author_facet Unrein, F.
Izaguirre, I.
Massana, R.
Balagué, V.
Gasol, J.M.
author_sort Unrein, F.
title Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
title_short Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
title_full Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
title_fullStr Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
title_full_unstemmed Nanoplankton assemblages in maritime Antarctic lakes: Characterisation and molecular fingerprinting comparison
title_sort nanoplankton assemblages in maritime antarctic lakes: characterisation and molecular fingerprinting comparison
url http://hdl.handle.net/20.500.12110/paper_09483055_v40_n3_p269_Unrein
work_keys_str_mv AT unreinf nanoplanktonassemblagesinmaritimeantarcticlakescharacterisationandmolecularfingerprintingcomparison
AT izaguirrei nanoplanktonassemblagesinmaritimeantarcticlakescharacterisationandmolecularfingerprintingcomparison
AT massanar nanoplanktonassemblagesinmaritimeantarcticlakescharacterisationandmolecularfingerprintingcomparison
AT balaguev nanoplanktonassemblagesinmaritimeantarcticlakescharacterisationandmolecularfingerprintingcomparison
AT gasoljm nanoplanktonassemblagesinmaritimeantarcticlakescharacterisationandmolecularfingerprintingcomparison
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