A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region

A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluoresc...

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Autores principales: Samaniego Lopez, C., Hebe Martínez, J., Uhrig, M.L., Coluccio Leskow, F., Spagnuolo, C.C.
Formato: JOUR
Materias:
boronolectin
cyanine
dyes/pigments
fluorescent probes
glycoprotein
Fluorescence
Glycoproteins
Infrared devices
Boronolectin
Cyanine
Dyes/pigments
Excellent solubility
Fluorescent probes
Fluorescent reporter
Near infrared region
Near-infrared emissions
Probes
boron derivative
fluorescent dye
mucin
chemical structure
chemistry
drug effect
epithelium cell
human
near infrared spectroscopy
procedures
Boron Compounds
Epithelial Cells
Fluorescent Dyes
Humans
Molecular Structure
Mucins
Spectroscopy, Near-Infrared
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09476539_v24_n24_p6344_SamaniegoLopez
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spelling todo:paper_09476539_v24_n24_p6344_SamaniegoLopez2023-10-03T15:49:31Z A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region Samaniego Lopez, C. Hebe Martínez, J. Uhrig, M.L. Coluccio Leskow, F. Spagnuolo, C.C. boronolectin cyanine dyes/pigments fluorescent probes glycoprotein Fluorescence Glycoproteins Infrared devices Boronolectin Cyanine Dyes/pigments Excellent solubility Fluorescent probes Fluorescent reporter Near infrared region Near-infrared emissions Probes boron derivative fluorescent dye glycoprotein mucin chemical structure chemistry drug effect epithelium cell human near infrared spectroscopy procedures Boron Compounds Epithelial Cells Fluorescent Dyes Glycoproteins Humans Molecular Structure Mucins Spectroscopy, Near-Infrared A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluorescent reporter with a linked benzoboroxole as the recognition unit, which operates through a dynamic covalent reaction between the boronic hemiester residue of the receptor and cis-diols of the analyte. The superior selectivity of the probe is displayed by the labeling of mucins present in Calu-3 cells. The new benzoboroxole fluorescent derivative gathers together key properties to make it a highly rated molecular probe: specificity, excellent solubility in water, and off–on near infrared emission. This probe is expected to be an excellent tool for imaging intracellular mucin to evaluate mucus-related diseases as well as a sensing strategy towards glycosylated structures with a high potential for theranostics approaches in biological samples. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic boronolectin
cyanine
dyes/pigments
fluorescent probes
glycoprotein
Fluorescence
Glycoproteins
Infrared devices
Boronolectin
Cyanine
Dyes/pigments
Excellent solubility
Fluorescent probes
Fluorescent reporter
Near infrared region
Near-infrared emissions
Probes
boron derivative
fluorescent dye
glycoprotein
mucin
chemical structure
chemistry
drug effect
epithelium cell
human
near infrared spectroscopy
procedures
Boron Compounds
Epithelial Cells
Fluorescent Dyes
Glycoproteins
Humans
Molecular Structure
Mucins
Spectroscopy, Near-Infrared
spellingShingle boronolectin
cyanine
dyes/pigments
fluorescent probes
glycoprotein
Fluorescence
Glycoproteins
Infrared devices
Boronolectin
Cyanine
Dyes/pigments
Excellent solubility
Fluorescent probes
Fluorescent reporter
Near infrared region
Near-infrared emissions
Probes
boron derivative
fluorescent dye
glycoprotein
mucin
chemical structure
chemistry
drug effect
epithelium cell
human
near infrared spectroscopy
procedures
Boron Compounds
Epithelial Cells
Fluorescent Dyes
Glycoproteins
Humans
Molecular Structure
Mucins
Spectroscopy, Near-Infrared
Samaniego Lopez, C.
Hebe Martínez, J.
Uhrig, M.L.
Coluccio Leskow, F.
Spagnuolo, C.C.
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
topic_facet boronolectin
cyanine
dyes/pigments
fluorescent probes
glycoprotein
Fluorescence
Glycoproteins
Infrared devices
Boronolectin
Cyanine
Dyes/pigments
Excellent solubility
Fluorescent probes
Fluorescent reporter
Near infrared region
Near-infrared emissions
Probes
boron derivative
fluorescent dye
glycoprotein
mucin
chemical structure
chemistry
drug effect
epithelium cell
human
near infrared spectroscopy
procedures
Boron Compounds
Epithelial Cells
Fluorescent Dyes
Glycoproteins
Humans
Molecular Structure
Mucins
Spectroscopy, Near-Infrared
description A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluorescent reporter with a linked benzoboroxole as the recognition unit, which operates through a dynamic covalent reaction between the boronic hemiester residue of the receptor and cis-diols of the analyte. The superior selectivity of the probe is displayed by the labeling of mucins present in Calu-3 cells. The new benzoboroxole fluorescent derivative gathers together key properties to make it a highly rated molecular probe: specificity, excellent solubility in water, and off–on near infrared emission. This probe is expected to be an excellent tool for imaging intracellular mucin to evaluate mucus-related diseases as well as a sensing strategy towards glycosylated structures with a high potential for theranostics approaches in biological samples. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
format JOUR
author Samaniego Lopez, C.
Hebe Martínez, J.
Uhrig, M.L.
Coluccio Leskow, F.
Spagnuolo, C.C.
author_facet Samaniego Lopez, C.
Hebe Martínez, J.
Uhrig, M.L.
Coluccio Leskow, F.
Spagnuolo, C.C.
author_sort Samaniego Lopez, C.
title A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
title_short A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
title_full A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
title_fullStr A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
title_full_unstemmed A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
title_sort highly sensitive fluorogenic probe for imaging glycoproteins and mucine activity in live cells in the near-infrared region
url http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez
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