Antiviral properties of fucoidan fractions from Leathesia difformis.

Three fractions of fucoidans isolated from the brown seaweed Leathesia difformis (Ee, Ec and Ea) were found to be selective antiviral agents against herpes simplex virus (HSV) types 1 and 2 and human cytomegalovirus. Fraction Ea was the most active, with IC50 values in the range 0.5-1.9 microg/ml wi...

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Autores principales: Feldman, S.C., Reynaldi, S., Stortz, C.A., Cerezo, A.S., Damont, E.B.
Formato: JOUR
Materias:
anticoagulant agent
antivirus agent
fucoidin
polysaccharide
adsorption
animal
article
brown alga
cell line
cell survival
Cercopithecus
chemistry
drug effect
Herpes simplex virus 2
human
IC 50
physiology
seaweed
Vero cell
virology
Adsorption
Algae, Brown
Animals
Anticoagulants
Antiviral Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Herpesvirus 2, Human
Humans
Inhibitory Concentration 50
Polysaccharides
Seaweed
Vero Cells
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09447113_v6_n5_p335_Feldman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09447113_v6_n5_p335_Feldman
record_format dspace
spelling todo:paper_09447113_v6_n5_p335_Feldman2023-10-03T15:49:17Z Antiviral properties of fucoidan fractions from Leathesia difformis. Feldman, S.C. Reynaldi, S. Stortz, C.A. Cerezo, A.S. Damont, E.B. anticoagulant agent antivirus agent fucoidin polysaccharide adsorption animal article brown alga cell line cell survival Cercopithecus chemistry drug effect Herpes simplex virus 2 human IC 50 physiology seaweed Vero cell virology Adsorption Algae, Brown Animals Anticoagulants Antiviral Agents Cell Line Cell Survival Cercopithecus aethiops Herpesvirus 2, Human Humans Inhibitory Concentration 50 Polysaccharides Seaweed Vero Cells Three fractions of fucoidans isolated from the brown seaweed Leathesia difformis (Ee, Ec and Ea) were found to be selective antiviral agents against herpes simplex virus (HSV) types 1 and 2 and human cytomegalovirus. Fraction Ea was the most active, with IC50 values in the range 0.5-1.9 microg/ml without affecting cell viability at concentrations up to 400 microg/ml. The antiherpetic activity of Ea was assessed by three different methods, plaque reduction, inhibition of virus yield and prevention of HSV-2 induced shut-off of cell protein synthesis, demonstrating that the inhibitory effect was independent of the antiviral assay and the multiplicity of infection. The mode of action of Ea could be ascribed to an inhibitory action on virus adsorption. The fucoidans did not inhibit the blood coagulation process even at concentrations exceeding more than 100 times the IC50 value. Fil:Reynaldi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09447113_v6_n5_p335_Feldman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic anticoagulant agent
antivirus agent
fucoidin
polysaccharide
adsorption
animal
article
brown alga
cell line
cell survival
Cercopithecus
chemistry
drug effect
Herpes simplex virus 2
human
IC 50
physiology
seaweed
Vero cell
virology
Adsorption
Algae, Brown
Animals
Anticoagulants
Antiviral Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Herpesvirus 2, Human
Humans
Inhibitory Concentration 50
Polysaccharides
Seaweed
Vero Cells
spellingShingle anticoagulant agent
antivirus agent
fucoidin
polysaccharide
adsorption
animal
article
brown alga
cell line
cell survival
Cercopithecus
chemistry
drug effect
Herpes simplex virus 2
human
IC 50
physiology
seaweed
Vero cell
virology
Adsorption
Algae, Brown
Animals
Anticoagulants
Antiviral Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Herpesvirus 2, Human
Humans
Inhibitory Concentration 50
Polysaccharides
Seaweed
Vero Cells
Feldman, S.C.
Reynaldi, S.
Stortz, C.A.
Cerezo, A.S.
Damont, E.B.
Antiviral properties of fucoidan fractions from Leathesia difformis.
topic_facet anticoagulant agent
antivirus agent
fucoidin
polysaccharide
adsorption
animal
article
brown alga
cell line
cell survival
Cercopithecus
chemistry
drug effect
Herpes simplex virus 2
human
IC 50
physiology
seaweed
Vero cell
virology
Adsorption
Algae, Brown
Animals
Anticoagulants
Antiviral Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Herpesvirus 2, Human
Humans
Inhibitory Concentration 50
Polysaccharides
Seaweed
Vero Cells
description Three fractions of fucoidans isolated from the brown seaweed Leathesia difformis (Ee, Ec and Ea) were found to be selective antiviral agents against herpes simplex virus (HSV) types 1 and 2 and human cytomegalovirus. Fraction Ea was the most active, with IC50 values in the range 0.5-1.9 microg/ml without affecting cell viability at concentrations up to 400 microg/ml. The antiherpetic activity of Ea was assessed by three different methods, plaque reduction, inhibition of virus yield and prevention of HSV-2 induced shut-off of cell protein synthesis, demonstrating that the inhibitory effect was independent of the antiviral assay and the multiplicity of infection. The mode of action of Ea could be ascribed to an inhibitory action on virus adsorption. The fucoidans did not inhibit the blood coagulation process even at concentrations exceeding more than 100 times the IC50 value.
format JOUR
author Feldman, S.C.
Reynaldi, S.
Stortz, C.A.
Cerezo, A.S.
Damont, E.B.
author_facet Feldman, S.C.
Reynaldi, S.
Stortz, C.A.
Cerezo, A.S.
Damont, E.B.
author_sort Feldman, S.C.
title Antiviral properties of fucoidan fractions from Leathesia difformis.
title_short Antiviral properties of fucoidan fractions from Leathesia difformis.
title_full Antiviral properties of fucoidan fractions from Leathesia difformis.
title_fullStr Antiviral properties of fucoidan fractions from Leathesia difformis.
title_full_unstemmed Antiviral properties of fucoidan fractions from Leathesia difformis.
title_sort antiviral properties of fucoidan fractions from leathesia difformis.
url http://hdl.handle.net/20.500.12110/paper_09447113_v6_n5_p335_Feldman
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AT reynaldis antiviralpropertiesoffucoidanfractionsfromleathesiadifformis
AT stortzca antiviralpropertiesoffucoidanfractionsfromleathesiadifformis
AT cerezoas antiviralpropertiesoffucoidanfractionsfromleathesiadifformis
AT damonteb antiviralpropertiesoffucoidanfractionsfromleathesiadifformis
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