Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients
We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferat...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09395555_v81_n2_p80_Honegger |
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todo:paper_09395555_v81_n2_p80_Honegger2023-10-03T15:48:53Z Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients Honegger, A.E. Hofer, E.L. Barañao, R.I. Mackinlay, T.A. Mackinlay, D.A. Bullorsky, E.O. Bordenave, R.H. Chasseing, N.A. Cancer Fibroblasts Soluble factors fibronectin interleukin 1beta prostaglandin E2 transforming growth factor beta1 article biosynthesis bone marrow culture breast cancer cancer patient cell assay cell function cell level cell proliferation colony forming unit continuous culture controlled study culture medium enzyme linked immunosorbent assay fibroblast human human cell lung cancer major clinical study priority journal radioimmunoassay stroma cell Bone Marrow Cells Breast Neoplasms Carcinoma, Squamous Cell Cell Division Cells, Cultured Culture Media, Conditioned Dinoprostone Female Fibroblasts Fibronectins Humans Interleukin-1 Lung Neoplasms Transforming Growth Factor beta We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1β, TGF-β1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE2 by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1β were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57±221.61 pg/ml. Levels of TGF-β1 in BCP were lower than NC values (P<0.05). LCP and BCP had significantly decreased levels of fibronectin when compared to NC values (P<0.05 and P<0.01, respectively). Levels of PGE2 in LCP were higher compared to NC (P<0.01). In conclusion, BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1β, TGF-β1, fibronectin, and PGE2 production. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09395555_v81_n2_p80_Honegger |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cancer Fibroblasts Soluble factors fibronectin interleukin 1beta prostaglandin E2 transforming growth factor beta1 article biosynthesis bone marrow culture breast cancer cancer patient cell assay cell function cell level cell proliferation colony forming unit continuous culture controlled study culture medium enzyme linked immunosorbent assay fibroblast human human cell lung cancer major clinical study priority journal radioimmunoassay stroma cell Bone Marrow Cells Breast Neoplasms Carcinoma, Squamous Cell Cell Division Cells, Cultured Culture Media, Conditioned Dinoprostone Female Fibroblasts Fibronectins Humans Interleukin-1 Lung Neoplasms Transforming Growth Factor beta |
| spellingShingle |
Cancer Fibroblasts Soluble factors fibronectin interleukin 1beta prostaglandin E2 transforming growth factor beta1 article biosynthesis bone marrow culture breast cancer cancer patient cell assay cell function cell level cell proliferation colony forming unit continuous culture controlled study culture medium enzyme linked immunosorbent assay fibroblast human human cell lung cancer major clinical study priority journal radioimmunoassay stroma cell Bone Marrow Cells Breast Neoplasms Carcinoma, Squamous Cell Cell Division Cells, Cultured Culture Media, Conditioned Dinoprostone Female Fibroblasts Fibronectins Humans Interleukin-1 Lung Neoplasms Transforming Growth Factor beta Honegger, A.E. Hofer, E.L. Barañao, R.I. Mackinlay, T.A. Mackinlay, D.A. Bullorsky, E.O. Bordenave, R.H. Chasseing, N.A. Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| topic_facet |
Cancer Fibroblasts Soluble factors fibronectin interleukin 1beta prostaglandin E2 transforming growth factor beta1 article biosynthesis bone marrow culture breast cancer cancer patient cell assay cell function cell level cell proliferation colony forming unit continuous culture controlled study culture medium enzyme linked immunosorbent assay fibroblast human human cell lung cancer major clinical study priority journal radioimmunoassay stroma cell Bone Marrow Cells Breast Neoplasms Carcinoma, Squamous Cell Cell Division Cells, Cultured Culture Media, Conditioned Dinoprostone Female Fibroblasts Fibronectins Humans Interleukin-1 Lung Neoplasms Transforming Growth Factor beta |
| description |
We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1β, TGF-β1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE2 by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1β were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57±221.61 pg/ml. Levels of TGF-β1 in BCP were lower than NC values (P<0.05). LCP and BCP had significantly decreased levels of fibronectin when compared to NC values (P<0.05 and P<0.01, respectively). Levels of PGE2 in LCP were higher compared to NC (P<0.01). In conclusion, BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1β, TGF-β1, fibronectin, and PGE2 production. |
| format |
JOUR |
| author |
Honegger, A.E. Hofer, E.L. Barañao, R.I. Mackinlay, T.A. Mackinlay, D.A. Bullorsky, E.O. Bordenave, R.H. Chasseing, N.A. |
| author_facet |
Honegger, A.E. Hofer, E.L. Barañao, R.I. Mackinlay, T.A. Mackinlay, D.A. Bullorsky, E.O. Bordenave, R.H. Chasseing, N.A. |
| author_sort |
Honegger, A.E. |
| title |
Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| title_short |
Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| title_full |
Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| title_fullStr |
Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| title_full_unstemmed |
Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| title_sort |
interleukin-1 beta, transforming growth factor beta 1, prostaglandin e2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients |
| url |
http://hdl.handle.net/20.500.12110/paper_09395555_v81_n2_p80_Honegger |
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