Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabita...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09320113_v116_n10_p2813_Rossin |
| Aporte de: |
| id |
todo:paper_09320113_v116_n10_p2813_Rossin |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09320113_v116_n10_p2813_Rossin2023-10-03T15:48:20Z Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) Rossin, M.A. De Francesco, P.N. Timi, J.T. Autofluorescence Laser confocal images Monogenea Proteinase K treatment SDS treatment Three-dimensional reconstruction proteinase K Article autofluorescence body size Dactylogyridae female fluorescence microscopy image processing male morphology nonhuman priority journal structure analysis taxonomy three dimensional imaging animal Characiformes classification confocal microscopy fish disease gill isolation and purification lake nose cavity parasitology river South America three dimensional imaging trematode trematodiasis ultrastructure veterinary Animals Characiformes Fish Diseases Gills Image Processing, Computer-Assisted Imaging, Three-Dimensional Lakes Male Microscopy, Confocal Nasal Cavity Rivers South America Trematoda Trematode Infections Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabitats. Indeed, diagnostic characteristics as sclerites and male copulatory complex are generally less than 100-μm long and are essential for taxonomic description and identification of species. Here, a combination of simple and routine methods for three-dimensional morphological studies on hard structures is proposed for dactylogirids: SDS treatment for clarification of specimens and enzymatic digestion with proteinase K for freeing sclerotized structures, followed by laser confocal microscopy. This method is applicable to fresh or fixed specimens and does not require staining or dehydration. Indeed, stable autofluorescence emission is detectable at 500–530 nm for bars, anchors, and male copulatory complex when excited by argon laser. Advantages of this protocol over previous methodologies for taking laser confocal images are discussed. Open access software for image processing was used for three-dimensional reconstruction of sclerotized structures generating models and full 360° rotation videos. © 2017, Springer-Verlag GmbH Germany. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09320113_v116_n10_p2813_Rossin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Autofluorescence Laser confocal images Monogenea Proteinase K treatment SDS treatment Three-dimensional reconstruction proteinase K Article autofluorescence body size Dactylogyridae female fluorescence microscopy image processing male morphology nonhuman priority journal structure analysis taxonomy three dimensional imaging animal Characiformes classification confocal microscopy fish disease gill isolation and purification lake nose cavity parasitology river South America three dimensional imaging trematode trematodiasis ultrastructure veterinary Animals Characiformes Fish Diseases Gills Image Processing, Computer-Assisted Imaging, Three-Dimensional Lakes Male Microscopy, Confocal Nasal Cavity Rivers South America Trematoda Trematode Infections |
| spellingShingle |
Autofluorescence Laser confocal images Monogenea Proteinase K treatment SDS treatment Three-dimensional reconstruction proteinase K Article autofluorescence body size Dactylogyridae female fluorescence microscopy image processing male morphology nonhuman priority journal structure analysis taxonomy three dimensional imaging animal Characiformes classification confocal microscopy fish disease gill isolation and purification lake nose cavity parasitology river South America three dimensional imaging trematode trematodiasis ultrastructure veterinary Animals Characiformes Fish Diseases Gills Image Processing, Computer-Assisted Imaging, Three-Dimensional Lakes Male Microscopy, Confocal Nasal Cavity Rivers South America Trematoda Trematode Infections Rossin, M.A. De Francesco, P.N. Timi, J.T. Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| topic_facet |
Autofluorescence Laser confocal images Monogenea Proteinase K treatment SDS treatment Three-dimensional reconstruction proteinase K Article autofluorescence body size Dactylogyridae female fluorescence microscopy image processing male morphology nonhuman priority journal structure analysis taxonomy three dimensional imaging animal Characiformes classification confocal microscopy fish disease gill isolation and purification lake nose cavity parasitology river South America three dimensional imaging trematode trematodiasis ultrastructure veterinary Animals Characiformes Fish Diseases Gills Image Processing, Computer-Assisted Imaging, Three-Dimensional Lakes Male Microscopy, Confocal Nasal Cavity Rivers South America Trematoda Trematode Infections |
| description |
Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabitats. Indeed, diagnostic characteristics as sclerites and male copulatory complex are generally less than 100-μm long and are essential for taxonomic description and identification of species. Here, a combination of simple and routine methods for three-dimensional morphological studies on hard structures is proposed for dactylogirids: SDS treatment for clarification of specimens and enzymatic digestion with proteinase K for freeing sclerotized structures, followed by laser confocal microscopy. This method is applicable to fresh or fixed specimens and does not require staining or dehydration. Indeed, stable autofluorescence emission is detectable at 500–530 nm for bars, anchors, and male copulatory complex when excited by argon laser. Advantages of this protocol over previous methodologies for taking laser confocal images are discussed. Open access software for image processing was used for three-dimensional reconstruction of sclerotized structures generating models and full 360° rotation videos. © 2017, Springer-Verlag GmbH Germany. |
| format |
JOUR |
| author |
Rossin, M.A. De Francesco, P.N. Timi, J.T. |
| author_facet |
Rossin, M.A. De Francesco, P.N. Timi, J.T. |
| author_sort |
Rossin, M.A. |
| title |
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| title_short |
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| title_full |
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| title_fullStr |
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| title_full_unstemmed |
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea) |
| title_sort |
three-dimensional morphology of rigid structures as a tool for taxonomic studies of dactylogyridae (monogenea) |
| url |
http://hdl.handle.net/20.500.12110/paper_09320113_v116_n10_p2813_Rossin |
| work_keys_str_mv |
AT rossinma threedimensionalmorphologyofrigidstructuresasatoolfortaxonomicstudiesofdactylogyridaemonogenea AT defrancescopn threedimensionalmorphologyofrigidstructuresasatoolfortaxonomicstudiesofdactylogyridaemonogenea AT timijt threedimensionalmorphologyofrigidstructuresasatoolfortaxonomicstudiesofdactylogyridaemonogenea |
| _version_ |
1782030759797719040 |