Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isol...
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todo:paper_09311785_v164_n11-12_p1054_Spedaletti2023-10-03T15:48:11Z Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina Spedaletti, Y. Aparicio, M. Mercado Cárdenas, G. Rodriguero, M. Taboada, G. Aban, C. Sühring, S. Vizgarra, O. Galván, M. anastomosis group genetic diversity ITS sequencing morphology Phaseolus vulgaris virulence cultivar disease resistance fungal disease fungus genetic analysis genetic variation herb morphology pathogenicity virulence Argentina Fungi Phaseolus (angiosperm) Phaseolus vulgaris Thanatephorus cucumeris Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA-ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2-2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north-western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. © 2016 Blackwell Verlag GmbH Fil:Rodriguero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09311785_v164_n11-12_p1054_Spedaletti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
anastomosis group genetic diversity ITS sequencing morphology Phaseolus vulgaris virulence cultivar disease resistance fungal disease fungus genetic analysis genetic variation herb morphology pathogenicity virulence Argentina Fungi Phaseolus (angiosperm) Phaseolus vulgaris Thanatephorus cucumeris |
| spellingShingle |
anastomosis group genetic diversity ITS sequencing morphology Phaseolus vulgaris virulence cultivar disease resistance fungal disease fungus genetic analysis genetic variation herb morphology pathogenicity virulence Argentina Fungi Phaseolus (angiosperm) Phaseolus vulgaris Thanatephorus cucumeris Spedaletti, Y. Aparicio, M. Mercado Cárdenas, G. Rodriguero, M. Taboada, G. Aban, C. Sühring, S. Vizgarra, O. Galván, M. Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| topic_facet |
anastomosis group genetic diversity ITS sequencing morphology Phaseolus vulgaris virulence cultivar disease resistance fungal disease fungus genetic analysis genetic variation herb morphology pathogenicity virulence Argentina Fungi Phaseolus (angiosperm) Phaseolus vulgaris Thanatephorus cucumeris |
| description |
Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA-ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2-2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north-western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. © 2016 Blackwell Verlag GmbH |
| format |
JOUR |
| author |
Spedaletti, Y. Aparicio, M. Mercado Cárdenas, G. Rodriguero, M. Taboada, G. Aban, C. Sühring, S. Vizgarra, O. Galván, M. |
| author_facet |
Spedaletti, Y. Aparicio, M. Mercado Cárdenas, G. Rodriguero, M. Taboada, G. Aban, C. Sühring, S. Vizgarra, O. Galván, M. |
| author_sort |
Spedaletti, Y. |
| title |
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| title_short |
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| title_full |
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| title_fullStr |
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| title_full_unstemmed |
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina |
| title_sort |
genetic characterization and pathogenicity of rhizoctonia solani associated with common bean web blight in the main bean growing area of argentina |
| url |
http://hdl.handle.net/20.500.12110/paper_09311785_v164_n11-12_p1054_Spedaletti |
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