A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas
An enzyme linked immunosorbent assay (ELISA) was developed for detecting IgM and IgG antibodies against Trypanosoma cruzi in blood hank donors from endemic or nonendemic areas. A crude extract of trypomastigotes from cultures was used as antigen. A total of 494 serum samples from patients with acute...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p31_Aznar |
| Aporte de: |
| id |
todo:paper_09288244_v18_n1_p31_Aznar |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09288244_v18_n1_p31_Aznar2023-10-03T15:47:30Z A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas Aznar, C. Liegeard, P. Mariette, C. Lafon, S. Levin, M.J. Hontebeyrie, M. Blood bank screening Chases' disease diagnosis Sensitivity Specificity Transfusion Trypanosoma cruzi antigen detection argentina article blood bank chagas disease controlled study diagnostic accuracy enzyme linked immunosorbent assay human infection control major clinical study priority journal screening test trypanosoma cruzi trypanosomiasis trypomastigote Animals Antibodies, Protozoan Blood Donors Chagas Disease Endemic Diseases Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G Immunoglobulin M Sensitivity and Specificity Trypanosoma cruzi An enzyme linked immunosorbent assay (ELISA) was developed for detecting IgM and IgG antibodies against Trypanosoma cruzi in blood hank donors from endemic or nonendemic areas. A crude extract of trypomastigotes from cultures was used as antigen. A total of 494 serum samples from patients with acute, congenital, or chronic form of Chagas' disease, and from healthy French individuals were studied. The sensitivity of the ELISA was determined with 89 serum samples from chagasic patients and was evaluated to 98.8%. The specificity was determined with 405 serum samples from French blood transfusion centers donors and evaluated to 98.3%. Two hundred and eighty- five serum samples from blood donors from Argentina and Brazil were also tested. Furthermore, in order to assess the absence of cross-reactivity with other protozoan infections, we studied 86 serum samples including (i) 32 individuals with cutaneous leishmaniasis living in a T. cruzi endemic region of Bolivia, and (ii) 54 patients from nonendemic area for Chagas' disease, 19 of them with kala-azar and 35 others with malaria. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p31_Aznar |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Blood bank screening Chases' disease diagnosis Sensitivity Specificity Transfusion Trypanosoma cruzi antigen detection argentina article blood bank chagas disease controlled study diagnostic accuracy enzyme linked immunosorbent assay human infection control major clinical study priority journal screening test trypanosoma cruzi trypanosomiasis trypomastigote Animals Antibodies, Protozoan Blood Donors Chagas Disease Endemic Diseases Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G Immunoglobulin M Sensitivity and Specificity Trypanosoma cruzi |
| spellingShingle |
Blood bank screening Chases' disease diagnosis Sensitivity Specificity Transfusion Trypanosoma cruzi antigen detection argentina article blood bank chagas disease controlled study diagnostic accuracy enzyme linked immunosorbent assay human infection control major clinical study priority journal screening test trypanosoma cruzi trypanosomiasis trypomastigote Animals Antibodies, Protozoan Blood Donors Chagas Disease Endemic Diseases Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G Immunoglobulin M Sensitivity and Specificity Trypanosoma cruzi Aznar, C. Liegeard, P. Mariette, C. Lafon, S. Levin, M.J. Hontebeyrie, M. A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| topic_facet |
Blood bank screening Chases' disease diagnosis Sensitivity Specificity Transfusion Trypanosoma cruzi antigen detection argentina article blood bank chagas disease controlled study diagnostic accuracy enzyme linked immunosorbent assay human infection control major clinical study priority journal screening test trypanosoma cruzi trypanosomiasis trypomastigote Animals Antibodies, Protozoan Blood Donors Chagas Disease Endemic Diseases Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G Immunoglobulin M Sensitivity and Specificity Trypanosoma cruzi |
| description |
An enzyme linked immunosorbent assay (ELISA) was developed for detecting IgM and IgG antibodies against Trypanosoma cruzi in blood hank donors from endemic or nonendemic areas. A crude extract of trypomastigotes from cultures was used as antigen. A total of 494 serum samples from patients with acute, congenital, or chronic form of Chagas' disease, and from healthy French individuals were studied. The sensitivity of the ELISA was determined with 89 serum samples from chagasic patients and was evaluated to 98.8%. The specificity was determined with 405 serum samples from French blood transfusion centers donors and evaluated to 98.3%. Two hundred and eighty- five serum samples from blood donors from Argentina and Brazil were also tested. Furthermore, in order to assess the absence of cross-reactivity with other protozoan infections, we studied 86 serum samples including (i) 32 individuals with cutaneous leishmaniasis living in a T. cruzi endemic region of Bolivia, and (ii) 54 patients from nonendemic area for Chagas' disease, 19 of them with kala-azar and 35 others with malaria. |
| format |
JOUR |
| author |
Aznar, C. Liegeard, P. Mariette, C. Lafon, S. Levin, M.J. Hontebeyrie, M. |
| author_facet |
Aznar, C. Liegeard, P. Mariette, C. Lafon, S. Levin, M.J. Hontebeyrie, M. |
| author_sort |
Aznar, C. |
| title |
A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| title_short |
A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| title_full |
A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| title_fullStr |
A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| title_full_unstemmed |
A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| title_sort |
simple trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas |
| url |
http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p31_Aznar |
| work_keys_str_mv |
AT aznarc asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT liegeardp asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT mariettec asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT lafons asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT levinmj asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT hontebeyriem asimpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT aznarc simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT liegeardp simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT mariettec simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT lafons simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT levinmj simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas AT hontebeyriem simpletrypanosomacruzienzymelinkedimmunoassayforcontrolofhumaninfectioninnonendemicareas |
| _version_ |
1782028243387285504 |