Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)

Most of the European pear cultivars fail to develop a desirable texture when ripening on the tree. In order to identify some biochemical anomalies that might be associated with this phenomenon, the main differences in cell wall polysaccharides occurring during " Bartlett" pear on-tree ripe...

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Autores principales: Raffo, M.D., Ponce, N.M.A., Sozzi, G.O., Stortz, C.A., Vicente, A.R.
Formato: JOUR
Materias:
Firmness
Pear
Pectin
Ripening
Texture
Ara
Pyrus
Pyrus communis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09255214_v73_n_p72_Raffo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09255214_v73_n_p72_Raffo2023-10-03T15:46:20Z Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.) Raffo, M.D. Ponce, N.M.A. Sozzi, G.O. Stortz, C.A. Vicente, A.R. Firmness Pear Pectin Ripening Texture Ara Pyrus Pyrus communis Most of the European pear cultivars fail to develop a desirable texture when ripening on the tree. In order to identify some biochemical anomalies that might be associated with this phenomenon, the main differences in cell wall polysaccharides occurring during " Bartlett" pear on-tree ripening were evaluated. Fruit was harvested at 3 different ripening stages (119, 147 and 161 days after anthesis, DAA) namely unripe (UR) mid-ripe (MR) both developing normal texture upon ripening, and late-ripe (LR), which did not result in buttery and juicy fruit. The characterization of cell wall structure was accomplished by determining the monosaccharide composition, yields, and solubility of uronic acids and neutral sugars in fractions extracted by water (WSF), 0.05M CDTA (CSF), 0.1M Na 2CO 3 (NSF), 1M and 4M KOH (1KSF and 4KSF). The size exclusion profiles in all the isolated fractions were also determined. In the transition from the UR to the MR stage, glucose from the 4KSF and Ara from all other fractions became markedly solubilized. A slight increase in water-soluble pectin content and depolymerization of the 4KSF and CSF were also found during this period. From the MR to the LR stage, a glucan probably originating in cellulose turnover was found in the 4KSF, and (though at a lower rate) Ara solubilization continued. In contrast, the depolymerization of all wall fractions after day 147 was minimal, and a shift of water soluble pectins toward the CSF was detected. An imbalance in polyuronide degradation, including decreased depolymerization of pectin backbones, together with continuing removal of Ara from RG I, and/or other modifications increasing ionic interactions, may lead fruit which yield an abnormal, mealy texture upon ripening. © 2012 Elsevier B.V.. Fil:Sozzi, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09255214_v73_n_p72_Raffo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Firmness
Pear
Pectin
Ripening
Texture
Ara
Pyrus
Pyrus communis
spellingShingle Firmness
Pear
Pectin
Ripening
Texture
Ara
Pyrus
Pyrus communis
Raffo, M.D.
Ponce, N.M.A.
Sozzi, G.O.
Stortz, C.A.
Vicente, A.R.
Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
topic_facet Firmness
Pear
Pectin
Ripening
Texture
Ara
Pyrus
Pyrus communis
description Most of the European pear cultivars fail to develop a desirable texture when ripening on the tree. In order to identify some biochemical anomalies that might be associated with this phenomenon, the main differences in cell wall polysaccharides occurring during " Bartlett" pear on-tree ripening were evaluated. Fruit was harvested at 3 different ripening stages (119, 147 and 161 days after anthesis, DAA) namely unripe (UR) mid-ripe (MR) both developing normal texture upon ripening, and late-ripe (LR), which did not result in buttery and juicy fruit. The characterization of cell wall structure was accomplished by determining the monosaccharide composition, yields, and solubility of uronic acids and neutral sugars in fractions extracted by water (WSF), 0.05M CDTA (CSF), 0.1M Na 2CO 3 (NSF), 1M and 4M KOH (1KSF and 4KSF). The size exclusion profiles in all the isolated fractions were also determined. In the transition from the UR to the MR stage, glucose from the 4KSF and Ara from all other fractions became markedly solubilized. A slight increase in water-soluble pectin content and depolymerization of the 4KSF and CSF were also found during this period. From the MR to the LR stage, a glucan probably originating in cellulose turnover was found in the 4KSF, and (though at a lower rate) Ara solubilization continued. In contrast, the depolymerization of all wall fractions after day 147 was minimal, and a shift of water soluble pectins toward the CSF was detected. An imbalance in polyuronide degradation, including decreased depolymerization of pectin backbones, together with continuing removal of Ara from RG I, and/or other modifications increasing ionic interactions, may lead fruit which yield an abnormal, mealy texture upon ripening. © 2012 Elsevier B.V..
format JOUR
author Raffo, M.D.
Ponce, N.M.A.
Sozzi, G.O.
Stortz, C.A.
Vicente, A.R.
author_facet Raffo, M.D.
Ponce, N.M.A.
Sozzi, G.O.
Stortz, C.A.
Vicente, A.R.
author_sort Raffo, M.D.
title Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
title_short Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
title_full Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
title_fullStr Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
title_full_unstemmed Changes on the cell wall composition of tree-ripened " Bartlett" pears (Pyrus communis L.)
title_sort changes on the cell wall composition of tree-ripened " bartlett" pears (pyrus communis l.)
url http://hdl.handle.net/20.500.12110/paper_09255214_v73_n_p72_Raffo
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