Imaging transcription factors dynamics with advanced fluorescence microscopy methods
Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes re...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p60_Verneri |
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todo:paper_09254773_v154_n_p60_Verneri2023-10-03T15:46:19Z Imaging transcription factors dynamics with advanced fluorescence microscopy methods Verneri, P. Romero, J.J. De Rossi, M.C. Alvarez, Y. Oses, C. Guberman, A. Levi, V. transcription factor cell differentiation cell fate cell nucleus cell population cell proliferation cell self-renewal chromatin immunoprecipitation embryo development fluorescence microscopy fluorescence recovery after photobleaching gene expression gene targeting molecular dynamics nonhuman pluripotent stem cell priority journal Review Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcription machinery and regulatory components such as TFs are recruited dynamically on their target genes making it essential exploring their dynamics in living cells to understand the transcriptional output. Non-invasive and very sensitive fluorescence microscopy methods are making it possible visualizing the dynamics of TFs in living specimens, complementing the information extracted from studies in fixed specimens and bulk assays. In this work, we briefly describe the basis of these microscopy methods and review how they contributed to our knowledge of the function of TFs relevant to embryo development and cell differentiation in a variety of systems ranging from single cells to whole organisms. © 2018 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p60_Verneri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
transcription factor cell differentiation cell fate cell nucleus cell population cell proliferation cell self-renewal chromatin immunoprecipitation embryo development fluorescence microscopy fluorescence recovery after photobleaching gene expression gene targeting molecular dynamics nonhuman pluripotent stem cell priority journal Review |
| spellingShingle |
transcription factor cell differentiation cell fate cell nucleus cell population cell proliferation cell self-renewal chromatin immunoprecipitation embryo development fluorescence microscopy fluorescence recovery after photobleaching gene expression gene targeting molecular dynamics nonhuman pluripotent stem cell priority journal Review Verneri, P. Romero, J.J. De Rossi, M.C. Alvarez, Y. Oses, C. Guberman, A. Levi, V. Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| topic_facet |
transcription factor cell differentiation cell fate cell nucleus cell population cell proliferation cell self-renewal chromatin immunoprecipitation embryo development fluorescence microscopy fluorescence recovery after photobleaching gene expression gene targeting molecular dynamics nonhuman pluripotent stem cell priority journal Review |
| description |
Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcription machinery and regulatory components such as TFs are recruited dynamically on their target genes making it essential exploring their dynamics in living cells to understand the transcriptional output. Non-invasive and very sensitive fluorescence microscopy methods are making it possible visualizing the dynamics of TFs in living specimens, complementing the information extracted from studies in fixed specimens and bulk assays. In this work, we briefly describe the basis of these microscopy methods and review how they contributed to our knowledge of the function of TFs relevant to embryo development and cell differentiation in a variety of systems ranging from single cells to whole organisms. © 2018 Elsevier B.V. |
| format |
JOUR |
| author |
Verneri, P. Romero, J.J. De Rossi, M.C. Alvarez, Y. Oses, C. Guberman, A. Levi, V. |
| author_facet |
Verneri, P. Romero, J.J. De Rossi, M.C. Alvarez, Y. Oses, C. Guberman, A. Levi, V. |
| author_sort |
Verneri, P. |
| title |
Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| title_short |
Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| title_full |
Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| title_fullStr |
Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| title_full_unstemmed |
Imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| title_sort |
imaging transcription factors dynamics with advanced fluorescence microscopy methods |
| url |
http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p60_Verneri |
| work_keys_str_mv |
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