Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells

Redox homeostasis is vital for cellular functions and to prevent the detrimental consequences of oxidative stress. Pluripotent stem cells (PSCs) have an enhanced antioxidant system which supports the preservation of their genome. Besides, reactive oxygen species (ROS) are proposed to be involved in...

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Autores principales: Solari, C., Petrone, M.V., Vazquez Echegaray, C., Cosentino, M.S., Waisman, A., Francia, M., Barañao, L., Miriuka, S., Guberman, A.
Formato: JOUR
Materias:
Antioxidant defense system
Cu-Zn Sod
Sod1
Transcriptional regulation
copper zinc superoxide dismutase
messenger RNA
mitochondrial DNA
octamer transcription factor 4
short hairpin RNA
transcription factor NANOG
transcription factor Sox2
Article
binding site
blastocyst
cardiac muscle cell
cell differentiation
cell growth
cell nucleus
cell self-renewal
controlled study
cytoplasm
down regulation
embryonic stem cell
gene
gene expression
gene mutation
human
human cell
in vitro study
nerve cell
oxidative stress
priority journal
promoter region
protein expression
protein targeting
smooth muscle cell
sod1 gene
transcription regulation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p116_Solari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09254773_v154_n_p116_Solari2023-10-03T15:46:18Z Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells Solari, C. Petrone, M.V. Vazquez Echegaray, C. Cosentino, M.S. Waisman, A. Francia, M. Barañao, L. Miriuka, S. Guberman, A. Antioxidant defense system Cu-Zn Sod Sod1 Transcriptional regulation copper zinc superoxide dismutase messenger RNA mitochondrial DNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 Article binding site blastocyst cardiac muscle cell cell differentiation cell growth cell nucleus cell self-renewal controlled study cytoplasm down regulation embryonic stem cell gene gene expression gene mutation human human cell in vitro study nerve cell oxidative stress priority journal promoter region protein expression protein targeting smooth muscle cell sod1 gene transcription regulation Redox homeostasis is vital for cellular functions and to prevent the detrimental consequences of oxidative stress. Pluripotent stem cells (PSCs) have an enhanced antioxidant system which supports the preservation of their genome. Besides, reactive oxygen species (ROS) are proposed to be involved in both self-renewal maintenance and in differentiation in embryonic stem cells (ESCs). Increasing evidence shows that cellular systems related to the oxidative stress defense decline along differentiation of PSCs. Although redox homeostasis has been extensively studied for many years, the knowledge about the transcriptional regulation of the genes involved in these systems is still limited. In this work, we studied Sod1 gene modulation by the PSCs fundamental transcription factors Oct4, Sox2 and Nanog. We found that this gene, which is expressed in mouse ESCs (mESCs), was repressed when they were induced to differentiate. Accordingly, these factors induced Sod1 promoter activity in a trans-activation assay. Finally, Sod1 mRNA levels were reduced when Oct4, Sox2 and Nanog were down-regulated by a shRNA approach in mESCs. Taken together, we found that PSCs’ key transcription factors are involved in the modulation of Sod1 gene, suggesting a relationship between the pluripotency core and redox homeostasis in these cells. © 2018 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p116_Solari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antioxidant defense system
Cu-Zn Sod
Sod1
Transcriptional regulation
copper zinc superoxide dismutase
messenger RNA
mitochondrial DNA
octamer transcription factor 4
short hairpin RNA
transcription factor NANOG
transcription factor Sox2
Article
binding site
blastocyst
cardiac muscle cell
cell differentiation
cell growth
cell nucleus
cell self-renewal
controlled study
cytoplasm
down regulation
embryonic stem cell
gene
gene expression
gene mutation
human
human cell
in vitro study
nerve cell
oxidative stress
priority journal
promoter region
protein expression
protein targeting
smooth muscle cell
sod1 gene
transcription regulation
spellingShingle Antioxidant defense system
Cu-Zn Sod
Sod1
Transcriptional regulation
copper zinc superoxide dismutase
messenger RNA
mitochondrial DNA
octamer transcription factor 4
short hairpin RNA
transcription factor NANOG
transcription factor Sox2
Article
binding site
blastocyst
cardiac muscle cell
cell differentiation
cell growth
cell nucleus
cell self-renewal
controlled study
cytoplasm
down regulation
embryonic stem cell
gene
gene expression
gene mutation
human
human cell
in vitro study
nerve cell
oxidative stress
priority journal
promoter region
protein expression
protein targeting
smooth muscle cell
sod1 gene
transcription regulation
Solari, C.
Petrone, M.V.
Vazquez Echegaray, C.
Cosentino, M.S.
Waisman, A.
Francia, M.
Barañao, L.
Miriuka, S.
Guberman, A.
Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
topic_facet Antioxidant defense system
Cu-Zn Sod
Sod1
Transcriptional regulation
copper zinc superoxide dismutase
messenger RNA
mitochondrial DNA
octamer transcription factor 4
short hairpin RNA
transcription factor NANOG
transcription factor Sox2
Article
binding site
blastocyst
cardiac muscle cell
cell differentiation
cell growth
cell nucleus
cell self-renewal
controlled study
cytoplasm
down regulation
embryonic stem cell
gene
gene expression
gene mutation
human
human cell
in vitro study
nerve cell
oxidative stress
priority journal
promoter region
protein expression
protein targeting
smooth muscle cell
sod1 gene
transcription regulation
description Redox homeostasis is vital for cellular functions and to prevent the detrimental consequences of oxidative stress. Pluripotent stem cells (PSCs) have an enhanced antioxidant system which supports the preservation of their genome. Besides, reactive oxygen species (ROS) are proposed to be involved in both self-renewal maintenance and in differentiation in embryonic stem cells (ESCs). Increasing evidence shows that cellular systems related to the oxidative stress defense decline along differentiation of PSCs. Although redox homeostasis has been extensively studied for many years, the knowledge about the transcriptional regulation of the genes involved in these systems is still limited. In this work, we studied Sod1 gene modulation by the PSCs fundamental transcription factors Oct4, Sox2 and Nanog. We found that this gene, which is expressed in mouse ESCs (mESCs), was repressed when they were induced to differentiate. Accordingly, these factors induced Sod1 promoter activity in a trans-activation assay. Finally, Sod1 mRNA levels were reduced when Oct4, Sox2 and Nanog were down-regulated by a shRNA approach in mESCs. Taken together, we found that PSCs’ key transcription factors are involved in the modulation of Sod1 gene, suggesting a relationship between the pluripotency core and redox homeostasis in these cells. © 2018 Elsevier B.V.
format JOUR
author Solari, C.
Petrone, M.V.
Vazquez Echegaray, C.
Cosentino, M.S.
Waisman, A.
Francia, M.
Barañao, L.
Miriuka, S.
Guberman, A.
author_facet Solari, C.
Petrone, M.V.
Vazquez Echegaray, C.
Cosentino, M.S.
Waisman, A.
Francia, M.
Barañao, L.
Miriuka, S.
Guberman, A.
author_sort Solari, C.
title Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
title_short Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
title_full Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
title_fullStr Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
title_full_unstemmed Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
title_sort superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors oct4, sox2 and nanog in embryonic stem cells
url http://hdl.handle.net/20.500.12110/paper_09254773_v154_n_p116_Solari
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