Differential inhibitory action of two azoic compounds against arenaviruses

The action of five azo-based compounds against the arenaviruses Junin (JUNV) and Tacaribe (TCRV) was evaluated in vitro by a virus yield inhibition assay in Vero cells and a cell-free virion inactivation assay. The compound 2-azo-(1′-(2′-nitroso)naphthyl)-benzoate (ANNB) was the most effective inhib...

Descripción completa

Detalles Bibliográficos
Autores principales: García, C.C., Candurra, N.A., Damonte, E.B.
Formato: JOUR
Materias:
Antiviral activity
Arenavirus
Azoic compound
Junin virus
Tacaribe virus
Virion inactivation
2 azo[1' (2' nitroso)naphthyl]benzoate
antivirus agent
azo compound
azoformamide
benzoic acid derivative
chemical compound
unclassified drug
urea derivative
virus protein
article
chronotherapy
drug activity
drug efficacy
drug inhibition
drug mechanism
drug sensitivity
evaluation
IC 50
in vitro study
inhibition kinetics
nonhuman
priority journal
protein synthesis
target cell
temperature dependence
Vero cell
virion
virus assembly
virus infection
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09248579_v21_n4_p319_Garcia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09248579_v21_n4_p319_Garcia
record_format dspace
spelling todo:paper_09248579_v21_n4_p319_Garcia2023-10-03T15:46:04Z Differential inhibitory action of two azoic compounds against arenaviruses García, C.C. Candurra, N.A. Damonte, E.B. Antiviral activity Arenavirus Azoic compound Junin virus Tacaribe virus Virion inactivation 2 azo[1' (2' nitroso)naphthyl]benzoate antivirus agent azo compound azoformamide benzoic acid derivative chemical compound unclassified drug urea derivative virus protein Arenavirus article chronotherapy drug activity drug efficacy drug inhibition drug mechanism drug sensitivity evaluation IC 50 in vitro study inhibition kinetics nonhuman priority journal protein synthesis target cell temperature dependence Vero cell virion virus assembly virus infection The action of five azo-based compounds against the arenaviruses Junin (JUNV) and Tacaribe (TCRV) was evaluated in vitro by a virus yield inhibition assay in Vero cells and a cell-free virion inactivation assay. The compound 2-azo-(1′-(2′-nitroso)naphthyl)-benzoate (ANNB) was the most effective inhibitor of arenavirus production in Vero cells with EC50 (effective concentration 50%) values in the range 6.5-26.2 μM and without inactivating properties. By contrast, the azodicarbonamide (ADA) was very effective in inactivating both arenaviruses with IC50 (inactivating concentration 50%) values of 7.6 and 5.3 μM against JUNV and TCRV, respectively. The virucidal activity of ADA was time- and temperature-dependent. ANNB had no inhibitory action on virus binding or penetration of target cells and did not affect the synthesis of viral proteins. The most likely event susceptible to ANNB would be the process of intracellular virion assembly. © 2002 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09248579_v21_n4_p319_Garcia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral activity
Arenavirus
Azoic compound
Junin virus
Tacaribe virus
Virion inactivation
2 azo[1' (2' nitroso)naphthyl]benzoate
antivirus agent
azo compound
azoformamide
benzoic acid derivative
chemical compound
unclassified drug
urea derivative
virus protein
Arenavirus
article
chronotherapy
drug activity
drug efficacy
drug inhibition
drug mechanism
drug sensitivity
evaluation
IC 50
in vitro study
inhibition kinetics
nonhuman
priority journal
protein synthesis
target cell
temperature dependence
Vero cell
virion
virus assembly
virus infection
spellingShingle Antiviral activity
Arenavirus
Azoic compound
Junin virus
Tacaribe virus
Virion inactivation
2 azo[1' (2' nitroso)naphthyl]benzoate
antivirus agent
azo compound
azoformamide
benzoic acid derivative
chemical compound
unclassified drug
urea derivative
virus protein
Arenavirus
article
chronotherapy
drug activity
drug efficacy
drug inhibition
drug mechanism
drug sensitivity
evaluation
IC 50
in vitro study
inhibition kinetics
nonhuman
priority journal
protein synthesis
target cell
temperature dependence
Vero cell
virion
virus assembly
virus infection
García, C.C.
Candurra, N.A.
Damonte, E.B.
Differential inhibitory action of two azoic compounds against arenaviruses
topic_facet Antiviral activity
Arenavirus
Azoic compound
Junin virus
Tacaribe virus
Virion inactivation
2 azo[1' (2' nitroso)naphthyl]benzoate
antivirus agent
azo compound
azoformamide
benzoic acid derivative
chemical compound
unclassified drug
urea derivative
virus protein
Arenavirus
article
chronotherapy
drug activity
drug efficacy
drug inhibition
drug mechanism
drug sensitivity
evaluation
IC 50
in vitro study
inhibition kinetics
nonhuman
priority journal
protein synthesis
target cell
temperature dependence
Vero cell
virion
virus assembly
virus infection
description The action of five azo-based compounds against the arenaviruses Junin (JUNV) and Tacaribe (TCRV) was evaluated in vitro by a virus yield inhibition assay in Vero cells and a cell-free virion inactivation assay. The compound 2-azo-(1′-(2′-nitroso)naphthyl)-benzoate (ANNB) was the most effective inhibitor of arenavirus production in Vero cells with EC50 (effective concentration 50%) values in the range 6.5-26.2 μM and without inactivating properties. By contrast, the azodicarbonamide (ADA) was very effective in inactivating both arenaviruses with IC50 (inactivating concentration 50%) values of 7.6 and 5.3 μM against JUNV and TCRV, respectively. The virucidal activity of ADA was time- and temperature-dependent. ANNB had no inhibitory action on virus binding or penetration of target cells and did not affect the synthesis of viral proteins. The most likely event susceptible to ANNB would be the process of intracellular virion assembly. © 2002 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.
format JOUR
author García, C.C.
Candurra, N.A.
Damonte, E.B.
author_facet García, C.C.
Candurra, N.A.
Damonte, E.B.
author_sort García, C.C.
title Differential inhibitory action of two azoic compounds against arenaviruses
title_short Differential inhibitory action of two azoic compounds against arenaviruses
title_full Differential inhibitory action of two azoic compounds against arenaviruses
title_fullStr Differential inhibitory action of two azoic compounds against arenaviruses
title_full_unstemmed Differential inhibitory action of two azoic compounds against arenaviruses
title_sort differential inhibitory action of two azoic compounds against arenaviruses
url http://hdl.handle.net/20.500.12110/paper_09248579_v21_n4_p319_Garcia
work_keys_str_mv AT garciacc differentialinhibitoryactionoftwoazoiccompoundsagainstarenaviruses
AT candurrana differentialinhibitoryactionoftwoazoiccompoundsagainstarenaviruses
AT damonteeb differentialinhibitoryactionoftwoazoiccompoundsagainstarenaviruses
_version_ 1807322533151637504

Ejemplares similares