Antiviral activity of enterocin CRL35 against herpesviruses

Enterocin CRL35 is an antibacterial polypeptide of 3.5x103 Da produced by Enterococcus faecium CRL35. A series of experiments are described that show the enterocin also had antiviral activity against thymidine-kinase positive (tk+) and deficient (tk-) strains of herpes simplex (HSV) type 1 and 2 in...

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Autores principales: Wachsman, M.O.B., Farías, M.E., Takeda, E., Sesma, F., De Ruiz Holgado, A.P., De Torres, R.A., Coto, C.E.
Formato: JOUR
Materias:
Antiviral
Bacteriocin
Enterococcus faecium
Herpes simplex type 1 and 2
bacteriocin
enterocin crl35
polypeptide
unclassified drug
animal cell
antiviral activity
article
cell strain bhk
controlled study
cytotoxicity
enterococcus faecium
hamster
herpes simplex virus 1
herpes simplex virus 2
herpes virus
monkey
nonhuman
priority journal
vero cell
virogenesis
Acyclovir
Animals
Antiviral Agents
Bacteriocins
Cell Line
Cell Survival
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Cricetinae
Herpesvirus 1, Human
Herpesvirus 2, Human
Vero Cells
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09248579_v12_n4_p293_Wachsman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09248579_v12_n4_p293_Wachsman
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spelling todo:paper_09248579_v12_n4_p293_Wachsman2023-10-03T15:46:04Z Antiviral activity of enterocin CRL35 against herpesviruses Wachsman, M.O.B. Farías, M.E. Takeda, E. Sesma, F. De Ruiz Holgado, A.P. De Torres, R.A. Coto, C.E. Antiviral Bacteriocin Enterococcus faecium Herpes simplex type 1 and 2 bacteriocin enterocin crl35 polypeptide unclassified drug animal cell antiviral activity article cell strain bhk controlled study cytotoxicity enterococcus faecium hamster herpes simplex virus 1 herpes simplex virus 2 herpes virus monkey nonhuman priority journal vero cell virogenesis Acyclovir Animals Antiviral Agents Bacteriocins Cell Line Cell Survival Cercopithecus aethiops Chromatography, High Pressure Liquid Cricetinae Herpesvirus 1, Human Herpesvirus 2, Human Vero Cells Enterocin CRL35 is an antibacterial polypeptide of 3.5x103 Da produced by Enterococcus faecium CRL35. A series of experiments are described that show the enterocin also had antiviral activity against thymidine-kinase positive (tk+) and deficient (tk-) strains of herpes simplex (HSV) type 1 and 2 in Vero and BHK-21 cells. This activity was observed at 100 μg/ml, 15-fold lower than the cytotoxic concentration. In both cell lines there was a 2 log inhibition of infectivity. The compound inhibited viral multiplication in a dose-dependent manner and had no virucidal effect. Enterocin CRL35 also inhibited the virion-associated host shutoff in infected Vero cells showing that intracellular viral multiplication was affected. Copyright (C) 1999 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09248579_v12_n4_p293_Wachsman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral
Bacteriocin
Enterococcus faecium
Herpes simplex type 1 and 2
bacteriocin
enterocin crl35
polypeptide
unclassified drug
animal cell
antiviral activity
article
cell strain bhk
controlled study
cytotoxicity
enterococcus faecium
hamster
herpes simplex virus 1
herpes simplex virus 2
herpes virus
monkey
nonhuman
priority journal
vero cell
virogenesis
Acyclovir
Animals
Antiviral Agents
Bacteriocins
Cell Line
Cell Survival
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Cricetinae
Herpesvirus 1, Human
Herpesvirus 2, Human
Vero Cells
spellingShingle Antiviral
Bacteriocin
Enterococcus faecium
Herpes simplex type 1 and 2
bacteriocin
enterocin crl35
polypeptide
unclassified drug
animal cell
antiviral activity
article
cell strain bhk
controlled study
cytotoxicity
enterococcus faecium
hamster
herpes simplex virus 1
herpes simplex virus 2
herpes virus
monkey
nonhuman
priority journal
vero cell
virogenesis
Acyclovir
Animals
Antiviral Agents
Bacteriocins
Cell Line
Cell Survival
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Cricetinae
Herpesvirus 1, Human
Herpesvirus 2, Human
Vero Cells
Wachsman, M.O.B.
Farías, M.E.
Takeda, E.
Sesma, F.
De Ruiz Holgado, A.P.
De Torres, R.A.
Coto, C.E.
Antiviral activity of enterocin CRL35 against herpesviruses
topic_facet Antiviral
Bacteriocin
Enterococcus faecium
Herpes simplex type 1 and 2
bacteriocin
enterocin crl35
polypeptide
unclassified drug
animal cell
antiviral activity
article
cell strain bhk
controlled study
cytotoxicity
enterococcus faecium
hamster
herpes simplex virus 1
herpes simplex virus 2
herpes virus
monkey
nonhuman
priority journal
vero cell
virogenesis
Acyclovir
Animals
Antiviral Agents
Bacteriocins
Cell Line
Cell Survival
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Cricetinae
Herpesvirus 1, Human
Herpesvirus 2, Human
Vero Cells
description Enterocin CRL35 is an antibacterial polypeptide of 3.5x103 Da produced by Enterococcus faecium CRL35. A series of experiments are described that show the enterocin also had antiviral activity against thymidine-kinase positive (tk+) and deficient (tk-) strains of herpes simplex (HSV) type 1 and 2 in Vero and BHK-21 cells. This activity was observed at 100 μg/ml, 15-fold lower than the cytotoxic concentration. In both cell lines there was a 2 log inhibition of infectivity. The compound inhibited viral multiplication in a dose-dependent manner and had no virucidal effect. Enterocin CRL35 also inhibited the virion-associated host shutoff in infected Vero cells showing that intracellular viral multiplication was affected. Copyright (C) 1999 Elsevier Science B.V.
format JOUR
author Wachsman, M.O.B.
Farías, M.E.
Takeda, E.
Sesma, F.
De Ruiz Holgado, A.P.
De Torres, R.A.
Coto, C.E.
author_facet Wachsman, M.O.B.
Farías, M.E.
Takeda, E.
Sesma, F.
De Ruiz Holgado, A.P.
De Torres, R.A.
Coto, C.E.
author_sort Wachsman, M.O.B.
title Antiviral activity of enterocin CRL35 against herpesviruses
title_short Antiviral activity of enterocin CRL35 against herpesviruses
title_full Antiviral activity of enterocin CRL35 against herpesviruses
title_fullStr Antiviral activity of enterocin CRL35 against herpesviruses
title_full_unstemmed Antiviral activity of enterocin CRL35 against herpesviruses
title_sort antiviral activity of enterocin crl35 against herpesviruses
url http://hdl.handle.net/20.500.12110/paper_09248579_v12_n4_p293_Wachsman
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AT fariasme antiviralactivityofenterocincrl35againstherpesviruses
AT takedae antiviralactivityofenterocincrl35againstherpesviruses
AT sesmaf antiviralactivityofenterocincrl35againstherpesviruses
AT deruizholgadoap antiviralactivityofenterocincrl35againstherpesviruses
AT detorresra antiviralactivityofenterocincrl35againstherpesviruses
AT cotoce antiviralactivityofenterocincrl35againstherpesviruses
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