Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study
The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-ad...
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todo:paper_09201742_v35_n3_p317_Devincenti2023-10-03T15:44:46Z Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study Devincenti, C.V. Díaz, A.O. García, A.M. Goldemberg, A.L. Muscle histochemistry Pectoral fibres Subcarangiform swimming Teleost TEM White croaker animal article cytochemistry cytology forelimb histology methodology muscle contraction Perciformes physiology skeletal muscle staining transmission electron microscopy ultrastructure Animals Forelimb Histocytochemistry Microscopy, Electron, Transmission Muscle Contraction Muscle Fibers, Skeletal Perciformes Staining and Labeling Genyonemus lineatus Micropogonias furnieri Teleostei The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-adenosintriphosphatase (mATPase) pre-incubated at alkaline and acid pHs were used to visualize the contraction velocity. Three zones were determined: superficial (SZ), medium (MZ) and deep (DZ). Staining for SDH, PAS and Sudan Black was positive only in the SZ. The level of alkaline mATPase was the highest in fibres from the DZ, intermediate in the MZ and low in the SZ; at an acid pH, the reverse was obtained. Fibres from the SZ were small with large quantities of subsarcolemmal mitochondria, scarce intermyofibrilar mitochondria and a well-developed sarcoplasmic reticulum; the myofibrils displayed a polygonal distribution along the entire length of the fibre. Fibres in the MZ were larger than those in the SZ, the myofibrils were densely packed, mitochondria prevailed under the sarcolemma and the sarcoplasmic reticulum was not abundant. Fibres from the DZ were the largest, with ribbon-shaped myofibrils and scarce mitochondria. The intercellular space was abundant and nervous endings were frequently observed. © Springer Science+Business Media B.V. 2008. Fil:Goldemberg, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09201742_v35_n3_p317_Devincenti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Muscle histochemistry Pectoral fibres Subcarangiform swimming Teleost TEM White croaker animal article cytochemistry cytology forelimb histology methodology muscle contraction Perciformes physiology skeletal muscle staining transmission electron microscopy ultrastructure Animals Forelimb Histocytochemistry Microscopy, Electron, Transmission Muscle Contraction Muscle Fibers, Skeletal Perciformes Staining and Labeling Genyonemus lineatus Micropogonias furnieri Teleostei |
| spellingShingle |
Muscle histochemistry Pectoral fibres Subcarangiform swimming Teleost TEM White croaker animal article cytochemistry cytology forelimb histology methodology muscle contraction Perciformes physiology skeletal muscle staining transmission electron microscopy ultrastructure Animals Forelimb Histocytochemistry Microscopy, Electron, Transmission Muscle Contraction Muscle Fibers, Skeletal Perciformes Staining and Labeling Genyonemus lineatus Micropogonias furnieri Teleostei Devincenti, C.V. Díaz, A.O. García, A.M. Goldemberg, A.L. Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| topic_facet |
Muscle histochemistry Pectoral fibres Subcarangiform swimming Teleost TEM White croaker animal article cytochemistry cytology forelimb histology methodology muscle contraction Perciformes physiology skeletal muscle staining transmission electron microscopy ultrastructure Animals Forelimb Histocytochemistry Microscopy, Electron, Transmission Muscle Contraction Muscle Fibers, Skeletal Perciformes Staining and Labeling Genyonemus lineatus Micropogonias furnieri Teleostei |
| description |
The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-adenosintriphosphatase (mATPase) pre-incubated at alkaline and acid pHs were used to visualize the contraction velocity. Three zones were determined: superficial (SZ), medium (MZ) and deep (DZ). Staining for SDH, PAS and Sudan Black was positive only in the SZ. The level of alkaline mATPase was the highest in fibres from the DZ, intermediate in the MZ and low in the SZ; at an acid pH, the reverse was obtained. Fibres from the SZ were small with large quantities of subsarcolemmal mitochondria, scarce intermyofibrilar mitochondria and a well-developed sarcoplasmic reticulum; the myofibrils displayed a polygonal distribution along the entire length of the fibre. Fibres in the MZ were larger than those in the SZ, the myofibrils were densely packed, mitochondria prevailed under the sarcolemma and the sarcoplasmic reticulum was not abundant. Fibres from the DZ were the largest, with ribbon-shaped myofibrils and scarce mitochondria. The intercellular space was abundant and nervous endings were frequently observed. © Springer Science+Business Media B.V. 2008. |
| format |
JOUR |
| author |
Devincenti, C.V. Díaz, A.O. García, A.M. Goldemberg, A.L. |
| author_facet |
Devincenti, C.V. Díaz, A.O. García, A.M. Goldemberg, A.L. |
| author_sort |
Devincenti, C.V. |
| title |
Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| title_short |
Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| title_full |
Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| title_fullStr |
Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| title_full_unstemmed |
Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study |
| title_sort |
pectoral fins of micropogonias furnieri: a histochemical and ultrastructural study |
| url |
http://hdl.handle.net/20.500.12110/paper_09201742_v35_n3_p317_Devincenti |
| work_keys_str_mv |
AT devincenticv pectoralfinsofmicropogoniasfurnieriahistochemicalandultrastructuralstudy AT diazao pectoralfinsofmicropogoniasfurnieriahistochemicalandultrastructuralstudy AT garciaam pectoralfinsofmicropogoniasfurnieriahistochemicalandultrastructuralstudy AT goldembergal pectoralfinsofmicropogoniasfurnieriahistochemicalandultrastructuralstudy |
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