Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus
The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time-kill analysis methods. Results obtained by the checkerboard method showed that the...
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todo:paper_09186158_v40_n6_p758_Vitorino2023-10-03T15:44:44Z Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus Vitorino, G.P. Becerra, M.C. Barrera, G.D. Caira, M.R. Mazzieri, M.R. Ciprofloxacin Combination Cooperative behavior Fluoroquinolone Norfloxacin Sinergistic effect ciprofloxacin norfloxacin quinoline derived antiinfective agent reactive oxygen metabolite antiinfective agent quinolone derivative antibacterial activity antibiotic sensitivity Article controlled study drug effect drug efficacy drug mechanism drug potentiation Escherichia coli fractional inhibitory concentration index in vitro study minimum inhibitory concentration nonhuman protein blood level Staphylococcus aureus drug effects Escherichia coli growth, development and aging microbial sensitivity test Staphylococcus aureus Anti-Bacterial Agents Drug Synergism Escherichia coli Fluoroquinolones Microbial Sensitivity Tests Staphylococcus aureus The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time-kill analysis methods. Results obtained by the checkerboard method showed that the more effectives combinations against Escherichia coli were 0.0009 μg/mL CIP0.0312 μg/mL NOR and 0.0037 μg/mL CIP0.0075 μg/mL NOR with a FIC index of 0.62. For Staphylococcus aureus, the combination of 0.0625 μg/mL CIP0.2500 μg/mL NOR showed a synergistic effect, with a FIC index of 0.50. The results of the time-kill method demonstrated either indifference or additivity of the combinations 0.0009 μg/mL CIP+0.0312 μg/mL NOR, 0.0018 μg/mL CIP+0.0312 μg/mL NOR, 0.0037 μg/mL CIP+0.0075 μg/mL NOR and 0.0037 μg/mL CIP+0.0156 μg/mL NOR at 24 h against E. coli. The combination 0.0037 μg/mL CIP+0.0312 μg/mL NOR showed synergistic activity. All the analyzed combinations evidenced bactericidal effects at 4 h. The combinations 0.0625 μg/mL CIP+0.2500 μg/mL NOR and 0.0625 μg/mL CIP+0.0625 μg/mL NOR showed indifference or additivity against S. aureus. None of them generated bactericidal effect at 4 h. Moreover, this last equimolecular combination (equivalent to 1/4 minimum inhibitory concentration (MIC) CIP+1/16 MIC NOR) generated higher reduction of nitro blue tetrazolium than drugs alone. By another way, combinations not equimolecular of CIP and NOR assayed, generated less levels of reactive oxygen species (ROS) than the components alone. © 2017 The Pharmaceutical Society of Japan. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09186158_v40_n6_p758_Vitorino |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ciprofloxacin Combination Cooperative behavior Fluoroquinolone Norfloxacin Sinergistic effect ciprofloxacin norfloxacin quinoline derived antiinfective agent reactive oxygen metabolite antiinfective agent quinolone derivative antibacterial activity antibiotic sensitivity Article controlled study drug effect drug efficacy drug mechanism drug potentiation Escherichia coli fractional inhibitory concentration index in vitro study minimum inhibitory concentration nonhuman protein blood level Staphylococcus aureus drug effects Escherichia coli growth, development and aging microbial sensitivity test Staphylococcus aureus Anti-Bacterial Agents Drug Synergism Escherichia coli Fluoroquinolones Microbial Sensitivity Tests Staphylococcus aureus |
| spellingShingle |
Ciprofloxacin Combination Cooperative behavior Fluoroquinolone Norfloxacin Sinergistic effect ciprofloxacin norfloxacin quinoline derived antiinfective agent reactive oxygen metabolite antiinfective agent quinolone derivative antibacterial activity antibiotic sensitivity Article controlled study drug effect drug efficacy drug mechanism drug potentiation Escherichia coli fractional inhibitory concentration index in vitro study minimum inhibitory concentration nonhuman protein blood level Staphylococcus aureus drug effects Escherichia coli growth, development and aging microbial sensitivity test Staphylococcus aureus Anti-Bacterial Agents Drug Synergism Escherichia coli Fluoroquinolones Microbial Sensitivity Tests Staphylococcus aureus Vitorino, G.P. Becerra, M.C. Barrera, G.D. Caira, M.R. Mazzieri, M.R. Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| topic_facet |
Ciprofloxacin Combination Cooperative behavior Fluoroquinolone Norfloxacin Sinergistic effect ciprofloxacin norfloxacin quinoline derived antiinfective agent reactive oxygen metabolite antiinfective agent quinolone derivative antibacterial activity antibiotic sensitivity Article controlled study drug effect drug efficacy drug mechanism drug potentiation Escherichia coli fractional inhibitory concentration index in vitro study minimum inhibitory concentration nonhuman protein blood level Staphylococcus aureus drug effects Escherichia coli growth, development and aging microbial sensitivity test Staphylococcus aureus Anti-Bacterial Agents Drug Synergism Escherichia coli Fluoroquinolones Microbial Sensitivity Tests Staphylococcus aureus |
| description |
The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time-kill analysis methods. Results obtained by the checkerboard method showed that the more effectives combinations against Escherichia coli were 0.0009 μg/mL CIP0.0312 μg/mL NOR and 0.0037 μg/mL CIP0.0075 μg/mL NOR with a FIC index of 0.62. For Staphylococcus aureus, the combination of 0.0625 μg/mL CIP0.2500 μg/mL NOR showed a synergistic effect, with a FIC index of 0.50. The results of the time-kill method demonstrated either indifference or additivity of the combinations 0.0009 μg/mL CIP+0.0312 μg/mL NOR, 0.0018 μg/mL CIP+0.0312 μg/mL NOR, 0.0037 μg/mL CIP+0.0075 μg/mL NOR and 0.0037 μg/mL CIP+0.0156 μg/mL NOR at 24 h against E. coli. The combination 0.0037 μg/mL CIP+0.0312 μg/mL NOR showed synergistic activity. All the analyzed combinations evidenced bactericidal effects at 4 h. The combinations 0.0625 μg/mL CIP+0.2500 μg/mL NOR and 0.0625 μg/mL CIP+0.0625 μg/mL NOR showed indifference or additivity against S. aureus. None of them generated bactericidal effect at 4 h. Moreover, this last equimolecular combination (equivalent to 1/4 minimum inhibitory concentration (MIC) CIP+1/16 MIC NOR) generated higher reduction of nitro blue tetrazolium than drugs alone. By another way, combinations not equimolecular of CIP and NOR assayed, generated less levels of reactive oxygen species (ROS) than the components alone. © 2017 The Pharmaceutical Society of Japan. |
| format |
JOUR |
| author |
Vitorino, G.P. Becerra, M.C. Barrera, G.D. Caira, M.R. Mazzieri, M.R. |
| author_facet |
Vitorino, G.P. Becerra, M.C. Barrera, G.D. Caira, M.R. Mazzieri, M.R. |
| author_sort |
Vitorino, G.P. |
| title |
Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| title_short |
Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| title_full |
Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| title_fullStr |
Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| title_full_unstemmed |
Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus |
| title_sort |
cooperative behavior of fluoroquinolone combinations against escherichia coli and staphylococcus aureus |
| url |
http://hdl.handle.net/20.500.12110/paper_09186158_v40_n6_p758_Vitorino |
| work_keys_str_mv |
AT vitorinogp cooperativebehavioroffluoroquinolonecombinationsagainstescherichiacoliandstaphylococcusaureus AT becerramc cooperativebehavioroffluoroquinolonecombinationsagainstescherichiacoliandstaphylococcusaureus AT barreragd cooperativebehavioroffluoroquinolonecombinationsagainstescherichiacoliandstaphylococcusaureus AT cairamr cooperativebehavioroffluoroquinolonecombinationsagainstescherichiacoliandstaphylococcusaureus AT mazzierimr cooperativebehavioroffluoroquinolonecombinationsagainstescherichiacoliandstaphylococcusaureus |
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