Effects of staurosporine on ACTH-mediated stimulation of aldosterone production

Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6...

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Autores principales: Pecci, A., Romero, D.G., Gomez-Sanchez, C.E., Lantos, C.P., Cozza, E.N.
Formato: JOUR
Materias:
Adrenocorticotropin
Aldosterone
Staurosporine
aldosterone
bucladesine
cholera toxin
corticotropin
cyclic AMP
cyclic AMP dependent protein kinase
isobutylmethylxanthine
protein kinase
protein kinase C inhibitor
staurosporine
stimulatory guanine nucleotide binding protein
animal cell
animal tissue
article
controlled study
enzyme inhibition
hormone synthesis
male
nonhuman
priority journal
rat
second messenger
zona glomerulosa
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_08986568_v8_n5_p381_Pecci
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spelling todo:paper_08986568_v8_n5_p381_Pecci2023-10-03T15:44:03Z Effects of staurosporine on ACTH-mediated stimulation of aldosterone production Pecci, A. Romero, D.G. Gomez-Sanchez, C.E. Lantos, C.P. Cozza, E.N. Adrenocorticotropin Aldosterone Staurosporine aldosterone bucladesine cholera toxin corticotropin cyclic AMP cyclic AMP dependent protein kinase isobutylmethylxanthine protein kinase protein kinase C inhibitor staurosporine stimulatory guanine nucleotide binding protein animal cell animal tissue article controlled study enzyme inhibition hormone synthesis male nonhuman priority journal rat second messenger zona glomerulosa Animalia Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6 μM) of staurosporine inhibited dibutyryl-cAMP-and forskolin-induced stimulation of aldosterone production. cAMP levels were increased only with low concentrations of the PKC inhibitor. This latter increase was avoided by treatment with a maximal concentration of isobutylmethylxanthine (MIX). Our results suggest that: (1) second messengers other than cAMP are involved in ACTH action; (2) staurosporine inhibits different kinases involved in ACTH action in a dose dependent manner; (3) the protein kinase inhibited by high concentrations of staurosporine appears to be the cAMP dependent kinase, PKA; and (4) the protein kinase inhibited by low concentrations of staurosporine remains to be identified. This latter species is suggested as being involved in mediating ACTH-induced activation of Gs. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adrenocorticotropin
Aldosterone
Staurosporine
aldosterone
bucladesine
cholera toxin
corticotropin
cyclic AMP
cyclic AMP dependent protein kinase
isobutylmethylxanthine
protein kinase
protein kinase C inhibitor
staurosporine
stimulatory guanine nucleotide binding protein
animal cell
animal tissue
article
controlled study
enzyme inhibition
hormone synthesis
male
nonhuman
priority journal
rat
second messenger
zona glomerulosa
Animalia
spellingShingle Adrenocorticotropin
Aldosterone
Staurosporine
aldosterone
bucladesine
cholera toxin
corticotropin
cyclic AMP
cyclic AMP dependent protein kinase
isobutylmethylxanthine
protein kinase
protein kinase C inhibitor
staurosporine
stimulatory guanine nucleotide binding protein
animal cell
animal tissue
article
controlled study
enzyme inhibition
hormone synthesis
male
nonhuman
priority journal
rat
second messenger
zona glomerulosa
Animalia
Pecci, A.
Romero, D.G.
Gomez-Sanchez, C.E.
Lantos, C.P.
Cozza, E.N.
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
topic_facet Adrenocorticotropin
Aldosterone
Staurosporine
aldosterone
bucladesine
cholera toxin
corticotropin
cyclic AMP
cyclic AMP dependent protein kinase
isobutylmethylxanthine
protein kinase
protein kinase C inhibitor
staurosporine
stimulatory guanine nucleotide binding protein
animal cell
animal tissue
article
controlled study
enzyme inhibition
hormone synthesis
male
nonhuman
priority journal
rat
second messenger
zona glomerulosa
Animalia
description Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6 μM) of staurosporine inhibited dibutyryl-cAMP-and forskolin-induced stimulation of aldosterone production. cAMP levels were increased only with low concentrations of the PKC inhibitor. This latter increase was avoided by treatment with a maximal concentration of isobutylmethylxanthine (MIX). Our results suggest that: (1) second messengers other than cAMP are involved in ACTH action; (2) staurosporine inhibits different kinases involved in ACTH action in a dose dependent manner; (3) the protein kinase inhibited by high concentrations of staurosporine appears to be the cAMP dependent kinase, PKA; and (4) the protein kinase inhibited by low concentrations of staurosporine remains to be identified. This latter species is suggested as being involved in mediating ACTH-induced activation of Gs.
format JOUR
author Pecci, A.
Romero, D.G.
Gomez-Sanchez, C.E.
Lantos, C.P.
Cozza, E.N.
author_facet Pecci, A.
Romero, D.G.
Gomez-Sanchez, C.E.
Lantos, C.P.
Cozza, E.N.
author_sort Pecci, A.
title Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
title_short Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
title_full Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
title_fullStr Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
title_full_unstemmed Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
title_sort effects of staurosporine on acth-mediated stimulation of aldosterone production
url http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci
work_keys_str_mv AT peccia effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction
AT romerodg effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction
AT gomezsanchezce effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction
AT lantoscp effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction
AT cozzaen effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction
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