Urease functionalized silica: A biohybrid substrate to drive self-mineralization

A study was conducted to demonstrate the use of urease to drive HAP-like film deposition over the silica surface under mild conditions. The study used a synthetic monodispersed submicrometric silica spheres as a model substrate to simplify mass transport boundary conditions. Direct adsorption of ure...

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Autores principales: Ortega, I., Jobbágy, M., Ferrer, M.L., Del Monte, F.
Formato: JOUR
Materias:
Adsorption
Aldehydes
Amines
Coupling agents
Hazardous materials
Mineralogy
Polymers
Covalent attachments
Enzyme loadings
Film depositions
Functionalization
Functionalized silicas
Glutaraldehyde
Mass transports
Model substrates
Mono-dispersed
Nucleation and growths
Silica particles
Silica spheres
Silica surfaces
Silica
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_08974756_v20_n24_p7368_Ortega
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_08974756_v20_n24_p7368_Ortega
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spelling todo:paper_08974756_v20_n24_p7368_Ortega2023-10-03T15:43:52Z Urease functionalized silica: A biohybrid substrate to drive self-mineralization Ortega, I. Jobbágy, M. Ferrer, M.L. Del Monte, F. Adsorption Aldehydes Amines Coupling agents Hazardous materials Mineralogy Polymers Covalent attachments Enzyme loadings Film depositions Functionalization Functionalized silicas Glutaraldehyde Mass transports Model substrates Mono-dispersed Nucleation and growths Silica particles Silica spheres Silica surfaces Silica A study was conducted to demonstrate the use of urease to drive HAP-like film deposition over the silica surface under mild conditions. The study used a synthetic monodispersed submicrometric silica spheres as a model substrate to simplify mass transport boundary conditions. Direct adsorption of urease on the silica surface was carried out to achieve silica functionalization. Covalent anchorage was also used to perform proper active enzyme loadings on the silica surface without shielding it with polyelectrolytes. Amino-capped silica particles were synthesized and biofunctionalized with urease during the study using direct covalent attachment with glutaraldehyde as coupling agent. This method proved that urease functionalized silica can be used as an interface for nucleation and growth of HAP-like coatings under mild conditions and short aging times. Fil:Jobbágy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08974756_v20_n24_p7368_Ortega
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adsorption
Aldehydes
Amines
Coupling agents
Hazardous materials
Mineralogy
Polymers
Covalent attachments
Enzyme loadings
Film depositions
Functionalization
Functionalized silicas
Glutaraldehyde
Mass transports
Model substrates
Mono-dispersed
Nucleation and growths
Silica particles
Silica spheres
Silica surfaces
Silica
spellingShingle Adsorption
Aldehydes
Amines
Coupling agents
Hazardous materials
Mineralogy
Polymers
Covalent attachments
Enzyme loadings
Film depositions
Functionalization
Functionalized silicas
Glutaraldehyde
Mass transports
Model substrates
Mono-dispersed
Nucleation and growths
Silica particles
Silica spheres
Silica surfaces
Silica
Ortega, I.
Jobbágy, M.
Ferrer, M.L.
Del Monte, F.
Urease functionalized silica: A biohybrid substrate to drive self-mineralization
topic_facet Adsorption
Aldehydes
Amines
Coupling agents
Hazardous materials
Mineralogy
Polymers
Covalent attachments
Enzyme loadings
Film depositions
Functionalization
Functionalized silicas
Glutaraldehyde
Mass transports
Model substrates
Mono-dispersed
Nucleation and growths
Silica particles
Silica spheres
Silica surfaces
Silica
description A study was conducted to demonstrate the use of urease to drive HAP-like film deposition over the silica surface under mild conditions. The study used a synthetic monodispersed submicrometric silica spheres as a model substrate to simplify mass transport boundary conditions. Direct adsorption of urease on the silica surface was carried out to achieve silica functionalization. Covalent anchorage was also used to perform proper active enzyme loadings on the silica surface without shielding it with polyelectrolytes. Amino-capped silica particles were synthesized and biofunctionalized with urease during the study using direct covalent attachment with glutaraldehyde as coupling agent. This method proved that urease functionalized silica can be used as an interface for nucleation and growth of HAP-like coatings under mild conditions and short aging times.
format JOUR
author Ortega, I.
Jobbágy, M.
Ferrer, M.L.
Del Monte, F.
author_facet Ortega, I.
Jobbágy, M.
Ferrer, M.L.
Del Monte, F.
author_sort Ortega, I.
title Urease functionalized silica: A biohybrid substrate to drive self-mineralization
title_short Urease functionalized silica: A biohybrid substrate to drive self-mineralization
title_full Urease functionalized silica: A biohybrid substrate to drive self-mineralization
title_fullStr Urease functionalized silica: A biohybrid substrate to drive self-mineralization
title_full_unstemmed Urease functionalized silica: A biohybrid substrate to drive self-mineralization
title_sort urease functionalized silica: a biohybrid substrate to drive self-mineralization
url http://hdl.handle.net/20.500.12110/paper_08974756_v20_n24_p7368_Ortega
work_keys_str_mv AT ortegai ureasefunctionalizedsilicaabiohybridsubstratetodriveselfmineralization
AT jobbagym ureasefunctionalizedsilicaabiohybridsubstratetodriveselfmineralization
AT ferrerml ureasefunctionalizedsilicaabiohybridsubstratetodriveselfmineralization
AT delmontef ureasefunctionalizedsilicaabiohybridsubstratetodriveselfmineralization
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