Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione

The fungicide agents are a key component in the fruits and vegetables production. The Iprodione residues are one of the pesticide more frequently found in food products. The available data about the cytotoxicity of iprodione and its metabolites are scarce and do not allow characterization of its gen...

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Autores principales:	Chaufan, G., Galvano, C., Nieves, M., Mudry, M.D., Ríos De Molina, M.D.C., Andrioli, N.B.
Formato:	JOUR
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0893228X_v_n_p_Chaufan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0893228X_v_n_p_Chaufan
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spelling	todo:paper_0893228X_v_n_p_Chaufan2023-10-03T15:41:44Z Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione Chaufan, G. Galvano, C. Nieves, M. Mudry, M.D. Ríos De Molina, M.D.C. Andrioli, N.B. The fungicide agents are a key component in the fruits and vegetables production. The Iprodione residues are one of the pesticide more frequently found in food products. The available data about the cytotoxicity of iprodione and its metabolites are scarce and do not allow characterization of its genotoxic potential and define the risk assessment.The human larynx epidermoid carcinoma cell line (HEp-2) has been shown to be sensitive to the toxic effects of xenobiotics of different origin and have been often used in citotoxicity and genotoxicity studies. The purpose of this paper is to evaluate the induction of genotoxicity and the role of oxidative stress in HEp-2cell line by exposure to the IP. The MTT test for viability resulted in CL 50 85.86 (77.05-95.68) μg/mL of Iprodione. On the basis of this result, we proceeded to expose the cells to the sublethal concentrations (below the CL 50 ) during 24 h to analyze the mitotic index and nuclear division index in order to determine the subcytotoxic concentrations of IP which the genotoxicity was evaluated. The subcytotoxic concentrations of 7, 17, and 25 μg/mL IP induced aneugenic effects as micronuclei centromere positive whereas 17 μg/mL was a threshold for centromere negative micronuclei induction in HEp-2 cells. The abnormal mitosis was induced for exposition of Hep-2 cells to the three concentrations. According to the result obtained, citotoxicity and genotoxicity oxidative stress studies were performed in 1.5, 7.0, and 25 μg/mL of IP. The results showed that the GSH intracellular content, the SOD activity and the levels of oxidative damage of the proteins were affected lead to redox imbalance. The decreased in the SOD activity and protein oxidation were in according to the result obtained to genotoxicity, suggesting that different biological targets could be affected. © 2019 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0893228X_v_n_p_Chaufan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description	The fungicide agents are a key component in the fruits and vegetables production. The Iprodione residues are one of the pesticide more frequently found in food products. The available data about the cytotoxicity of iprodione and its metabolites are scarce and do not allow characterization of its genotoxic potential and define the risk assessment.The human larynx epidermoid carcinoma cell line (HEp-2) has been shown to be sensitive to the toxic effects of xenobiotics of different origin and have been often used in citotoxicity and genotoxicity studies. The purpose of this paper is to evaluate the induction of genotoxicity and the role of oxidative stress in HEp-2cell line by exposure to the IP. The MTT test for viability resulted in CL 50 85.86 (77.05-95.68) μg/mL of Iprodione. On the basis of this result, we proceeded to expose the cells to the sublethal concentrations (below the CL 50 ) during 24 h to analyze the mitotic index and nuclear division index in order to determine the subcytotoxic concentrations of IP which the genotoxicity was evaluated. The subcytotoxic concentrations of 7, 17, and 25 μg/mL IP induced aneugenic effects as micronuclei centromere positive whereas 17 μg/mL was a threshold for centromere negative micronuclei induction in HEp-2 cells. The abnormal mitosis was induced for exposition of Hep-2 cells to the three concentrations. According to the result obtained, citotoxicity and genotoxicity oxidative stress studies were performed in 1.5, 7.0, and 25 μg/mL of IP. The results showed that the GSH intracellular content, the SOD activity and the levels of oxidative damage of the proteins were affected lead to redox imbalance. The decreased in the SOD activity and protein oxidation were in according to the result obtained to genotoxicity, suggesting that different biological targets could be affected. © 2019 American Chemical Society.
format	JOUR
author	Chaufan, G. Galvano, C. Nieves, M. Mudry, M.D. Ríos De Molina, M.D.C. Andrioli, N.B.
spellingShingle	Chaufan, G. Galvano, C. Nieves, M. Mudry, M.D. Ríos De Molina, M.D.C. Andrioli, N.B. Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
author_facet	Chaufan, G. Galvano, C. Nieves, M. Mudry, M.D. Ríos De Molina, M.D.C. Andrioli, N.B.
author_sort	Chaufan, G.
title	Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
title_short	Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
title_full	Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
title_fullStr	Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
title_full_unstemmed	Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione
title_sort	oxidative response and micronucleus centromere assay in hep-2 cells exposed to fungicide iprodione
url	http://hdl.handle.net/20.500.12110/paper_0893228X_v_n_p_Chaufan
work_keys_str_mv	AT chaufang oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione AT galvanoc oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione AT nievesm oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione AT mudrymd oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione AT riosdemolinamdc oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione AT andriolinb oxidativeresponseandmicronucleuscentromereassayinhep2cellsexposedtofungicideiprodione
_version_	1782026217247997952

Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione

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