The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm du...
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todo:paper_08926638_v29_n10_p4189_Vasen2023-10-03T15:41:37Z The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization Vasen, G. Battistone, M.A. Croci, D.O. Brukman, N.G. Muñoz, M.W. Stupirski, J.C. Rabinovich, G.A. Cuasnicú, P.S. Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycandependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals12/2 ) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals12/2 sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals12/2 sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.-Vasen, G., Battistone, M. A., Croci, D. O., Brukman, N. G., Weigel Muńoz, M., Stupirski, J. C., Rabinovich, G. A., Cuasnicú, P. S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization. © FASEB. Fil:Croci, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Brukman, N.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cuasnicú, P.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08926638_v29_n10_p4189_Vasen |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis |
| spellingShingle |
Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis Vasen, G. Battistone, M.A. Croci, D.O. Brukman, N.G. Muñoz, M.W. Stupirski, J.C. Rabinovich, G.A. Cuasnicú, P.S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| topic_facet |
Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis |
| description |
Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycandependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals12/2 ) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals12/2 sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals12/2 sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.-Vasen, G., Battistone, M. A., Croci, D. O., Brukman, N. G., Weigel Muńoz, M., Stupirski, J. C., Rabinovich, G. A., Cuasnicú, P. S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization. © FASEB. |
| format |
JOUR |
| author |
Vasen, G. Battistone, M.A. Croci, D.O. Brukman, N.G. Muñoz, M.W. Stupirski, J.C. Rabinovich, G.A. Cuasnicú, P.S. |
| author_facet |
Vasen, G. Battistone, M.A. Croci, D.O. Brukman, N.G. Muñoz, M.W. Stupirski, J.C. Rabinovich, G.A. Cuasnicú, P.S. |
| author_sort |
Vasen, G. |
| title |
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| title_short |
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| title_full |
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| title_fullStr |
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| title_full_unstemmed |
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| title_sort |
galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization |
| url |
http://hdl.handle.net/20.500.12110/paper_08926638_v29_n10_p4189_Vasen |
| work_keys_str_mv |
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