Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage

Neural stem cells (NSCs) of the olfactory epithelium (OE) are responsible for tissue maintenance and the neural regeneration after severe damage of the tissue. In the normal OE, NSCs are located in the basal layer, olfactory receptor neurons (ORNs) mainly in the middle layer, and sustentacular (SUS)...

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Autores principales:	Frontera, J.L., Raices, M., Cervino, A.S., Pozzi, A.G., Paz, D.A.
Formato:	JOUR
Materias:	Neural stem cells Neurogenesis Olfaction Olfactory injury Olfactory receptor neurons zinc sulfate animal cell animal experiment animal model animal tissue Article basal cell basement membrane cell activation cell loss cell maturation cell proliferation cell regeneration cell structure cells cellular distribution concentration (parameters) controlled study convalescence embryo epithelium cell nerve cell differentiation nerve regeneration neural stem cell neuroepithelium nonhuman olfactory bulb olfactory epithelium olfactory nerve injury olfactory receptor priority journal sensory stimulation smelling sustentacular cell tadpole thickness tissue regeneration tissue structure Xenopus laevis animal cheek drug effects growth, development and aging nervous system development olfactory mucosa olfactory receptor neuron physiology Animals Cell Proliferation Cheek Nerve Regeneration Neural Stem Cells Olfactory Bulb Olfactory Mucosa Olfactory Receptor Neurons Zinc Sulfate
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08910618_v77_n_p1_Frontera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_08910618_v77_n_p1_Frontera
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spelling	todo:paper_08910618_v77_n_p1_Frontera2023-10-03T15:41:16Z Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage Frontera, J.L. Raices, M. Cervino, A.S. Pozzi, A.G. Paz, D.A. Neural stem cells Neurogenesis Olfaction Olfactory injury Olfactory receptor neurons zinc sulfate zinc sulfate animal cell animal experiment animal model animal tissue Article basal cell basement membrane cell activation cell loss cell maturation cell proliferation cell regeneration cell structure cells cellular distribution concentration (parameters) controlled study convalescence embryo epithelium cell nerve cell differentiation nerve regeneration neural stem cell neuroepithelium nonhuman olfactory bulb olfactory epithelium olfactory nerve injury olfactory receptor priority journal sensory stimulation smelling sustentacular cell tadpole thickness tissue regeneration tissue structure Xenopus laevis animal cheek drug effects growth, development and aging nervous system development olfactory mucosa olfactory receptor neuron physiology Animals Cell Proliferation Cheek Nerve Regeneration Neural Stem Cells Neurogenesis Olfactory Bulb Olfactory Mucosa Olfactory Receptor Neurons Xenopus laevis Zinc Sulfate Neural stem cells (NSCs) of the olfactory epithelium (OE) are responsible for tissue maintenance and the neural regeneration after severe damage of the tissue. In the normal OE, NSCs are located in the basal layer, olfactory receptor neurons (ORNs) mainly in the middle layer, and sustentacular (SUS) cells in the most apical olfactory layer. In this work, we induced severe damage of the OE through treatment with a zinc sulfate (ZnSO4) solution directly in the medium, which resulted in the loss of ORNs and SUS cells, but retention of the basal layer. During recovery following injury, the OE exhibited increased proliferation of NSCs and rapid neural regeneration. After 24 h of recovery, new ORNs and SUS cells were observed. Normal morphology and olfactory function were reached after 168 h (7 days) of recovery after ZnSO4 treatment. Taken together, these data support the hypothesis that NSCs in the basal layer activate after OE injury and that these are sufficient for complete neural regeneration and olfactory function restoration. Our analysis provides histological and functional insights into the dynamics between olfactory neurogenesis and the neuronal integration into the neuronal circuitry of the olfactory bulb that restores the function of the olfactory system. © 2016. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08910618_v77_n_p1_Frontera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Neural stem cells Neurogenesis Olfaction Olfactory injury Olfactory receptor neurons zinc sulfate zinc sulfate animal cell animal experiment animal model animal tissue Article basal cell basement membrane cell activation cell loss cell maturation cell proliferation cell regeneration cell structure cells cellular distribution concentration (parameters) controlled study convalescence embryo epithelium cell nerve cell differentiation nerve regeneration neural stem cell neuroepithelium nonhuman olfactory bulb olfactory epithelium olfactory nerve injury olfactory receptor priority journal sensory stimulation smelling sustentacular cell tadpole thickness tissue regeneration tissue structure Xenopus laevis animal cheek drug effects growth, development and aging nervous system development olfactory mucosa olfactory receptor neuron physiology Animals Cell Proliferation Cheek Nerve Regeneration Neural Stem Cells Neurogenesis Olfactory Bulb Olfactory Mucosa Olfactory Receptor Neurons Xenopus laevis Zinc Sulfate
spellingShingle	Neural stem cells Neurogenesis Olfaction Olfactory injury Olfactory receptor neurons zinc sulfate zinc sulfate animal cell animal experiment animal model animal tissue Article basal cell basement membrane cell activation cell loss cell maturation cell proliferation cell regeneration cell structure cells cellular distribution concentration (parameters) controlled study convalescence embryo epithelium cell nerve cell differentiation nerve regeneration neural stem cell neuroepithelium nonhuman olfactory bulb olfactory epithelium olfactory nerve injury olfactory receptor priority journal sensory stimulation smelling sustentacular cell tadpole thickness tissue regeneration tissue structure Xenopus laevis animal cheek drug effects growth, development and aging nervous system development olfactory mucosa olfactory receptor neuron physiology Animals Cell Proliferation Cheek Nerve Regeneration Neural Stem Cells Neurogenesis Olfactory Bulb Olfactory Mucosa Olfactory Receptor Neurons Xenopus laevis Zinc Sulfate Frontera, J.L. Raices, M. Cervino, A.S. Pozzi, A.G. Paz, D.A. Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
topic_facet	Neural stem cells Neurogenesis Olfaction Olfactory injury Olfactory receptor neurons zinc sulfate zinc sulfate animal cell animal experiment animal model animal tissue Article basal cell basement membrane cell activation cell loss cell maturation cell proliferation cell regeneration cell structure cells cellular distribution concentration (parameters) controlled study convalescence embryo epithelium cell nerve cell differentiation nerve regeneration neural stem cell neuroepithelium nonhuman olfactory bulb olfactory epithelium olfactory nerve injury olfactory receptor priority journal sensory stimulation smelling sustentacular cell tadpole thickness tissue regeneration tissue structure Xenopus laevis animal cheek drug effects growth, development and aging nervous system development olfactory mucosa olfactory receptor neuron physiology Animals Cell Proliferation Cheek Nerve Regeneration Neural Stem Cells Neurogenesis Olfactory Bulb Olfactory Mucosa Olfactory Receptor Neurons Xenopus laevis Zinc Sulfate
description	Neural stem cells (NSCs) of the olfactory epithelium (OE) are responsible for tissue maintenance and the neural regeneration after severe damage of the tissue. In the normal OE, NSCs are located in the basal layer, olfactory receptor neurons (ORNs) mainly in the middle layer, and sustentacular (SUS) cells in the most apical olfactory layer. In this work, we induced severe damage of the OE through treatment with a zinc sulfate (ZnSO4) solution directly in the medium, which resulted in the loss of ORNs and SUS cells, but retention of the basal layer. During recovery following injury, the OE exhibited increased proliferation of NSCs and rapid neural regeneration. After 24 h of recovery, new ORNs and SUS cells were observed. Normal morphology and olfactory function were reached after 168 h (7 days) of recovery after ZnSO4 treatment. Taken together, these data support the hypothesis that NSCs in the basal layer activate after OE injury and that these are sufficient for complete neural regeneration and olfactory function restoration. Our analysis provides histological and functional insights into the dynamics between olfactory neurogenesis and the neuronal integration into the neuronal circuitry of the olfactory bulb that restores the function of the olfactory system. © 2016.
format	JOUR
author	Frontera, J.L. Raices, M. Cervino, A.S. Pozzi, A.G. Paz, D.A.
author_facet	Frontera, J.L. Raices, M. Cervino, A.S. Pozzi, A.G. Paz, D.A.
author_sort	Frontera, J.L.
title	Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
title_short	Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
title_full	Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
title_fullStr	Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
title_full_unstemmed	Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
title_sort	neural regeneration dynamics of xenopus laevis olfactory epithelium after zinc sulfate-induced damage
url	http://hdl.handle.net/20.500.12110/paper_08910618_v77_n_p1_Frontera
work_keys_str_mv	AT fronterajl neuralregenerationdynamicsofxenopuslaevisolfactoryepitheliumafterzincsulfateinduceddamage AT raicesm neuralregenerationdynamicsofxenopuslaevisolfactoryepitheliumafterzincsulfateinduceddamage AT cervinoas neuralregenerationdynamicsofxenopuslaevisolfactoryepitheliumafterzincsulfateinduceddamage AT pozziag neuralregenerationdynamicsofxenopuslaevisolfactoryepitheliumafterzincsulfateinduceddamage AT pazda neuralregenerationdynamicsofxenopuslaevisolfactoryepitheliumafterzincsulfateinduceddamage
_version_	1807314724130390016

Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage

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