Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts

Heavy metals contamination has become an important risk factor for public health and the environment. Chromium is a frequent industrial contaminant and is also used in orthopaedic joint replacements made from cobalt-chromium-alloy. Since hexavalent chromium (Cr(VI)) was reported as genotoxic and car...

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Autores principales:	Martini, C.N., Brandani, J.N., Gabrielli, M., Vila, M.D.C.
Formato:	JOUR
Materias:	3T3-L1 fibroblasts Adipogenesis Hexavalent chromium PPAR gamma Proliferation chromium dexamethasone insulin isobutylmethylxanthine peroxisome proliferator activated receptor gamma triacylglycerol chromium hexavalent ion pollutant adipocyte animal cell article cell count cell differentiation cell expansion cell proliferation cell strain 3T3 comparative study controlled study cytotoxicity test embryo immunofluorescence lipid storage micronucleus mitotic clonal expansion molecular dynamics mouse nonhuman Western blotting 3T3 cell line animal cytology drug effects fibroblast physiology toxicity 3T3-L1 Cells Adipocytes Animals Cell Differentiation Cell Proliferation Chromium Environmental Pollutants Fibroblasts Mice
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08872333_v28_n4_p700_Martini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_08872333_v28_n4_p700_Martini2023-10-03T15:40:48Z Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts Martini, C.N. Brandani, J.N. Gabrielli, M. Vila, M.D.C. 3T3-L1 fibroblasts Adipogenesis Hexavalent chromium PPAR gamma Proliferation chromium dexamethasone insulin isobutylmethylxanthine peroxisome proliferator activated receptor gamma triacylglycerol chromium chromium hexavalent ion pollutant adipocyte animal cell article cell count cell differentiation cell expansion cell proliferation cell strain 3T3 comparative study controlled study cytotoxicity test embryo immunofluorescence lipid storage micronucleus mitotic clonal expansion molecular dynamics mouse nonhuman Western blotting 3T3 cell line adipocyte animal cell differentiation cell proliferation cytology drug effects fibroblast physiology pollutant toxicity 3T3-L1 Cells Adipocytes Animals Cell Differentiation Cell Proliferation Chromium Environmental Pollutants Fibroblasts Mice Heavy metals contamination has become an important risk factor for public health and the environment. Chromium is a frequent industrial contaminant and is also used in orthopaedic joint replacements made from cobalt-chromium-alloy. Since hexavalent chromium (Cr(VI)) was reported as genotoxic and carcinogenic in different mammals, to further evaluate its cytotoxicity, we investigated the effect of this heavy metal in the proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. These cells, after the addition of a mixture containing insulin, dexamethasone and methylisobutylxanthine, first proliferate, a process known as mitotic clonal expansion (MCE), and then differentiate to adipocytes. In this differentiation process a key transcription factor is induced: peroxisome proliferator-activated receptor gamma (PPAR gamma). We found that treatment of 3T3-L1 fibroblasts with potassium chromate inhibited proliferation in exponentially growing cells and MCE as well as differentiation. A decrease in PPAR gamma content, evaluated by western blot and immunofluorescence, was found in cells differentiated in the presence of chromium. On the other hand, after inhibition of differentiation with chromium, when the metal was removed, differentiation was recovered, which indicates that this may be a reversible effect. We also found an increase in the number of micronucleated cells after treatment with Cr(VI) which is associated with genotoxic effects. According to our results, Cr(VI) is able to inhibit proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts and to increase micronucleated cells, which are all indicative of alterations in cellular physiology and therefore, contributes to further elucidate the cytotoxic effects of this heavy metal. © 2014 Elsevier Ltd. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabrielli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08872333_v28_n4_p700_Martini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3-L1 fibroblasts Adipogenesis Hexavalent chromium PPAR gamma Proliferation chromium dexamethasone insulin isobutylmethylxanthine peroxisome proliferator activated receptor gamma triacylglycerol chromium chromium hexavalent ion pollutant adipocyte animal cell article cell count cell differentiation cell expansion cell proliferation cell strain 3T3 comparative study controlled study cytotoxicity test embryo immunofluorescence lipid storage micronucleus mitotic clonal expansion molecular dynamics mouse nonhuman Western blotting 3T3 cell line adipocyte animal cell differentiation cell proliferation cytology drug effects fibroblast physiology pollutant toxicity 3T3-L1 Cells Adipocytes Animals Cell Differentiation Cell Proliferation Chromium Environmental Pollutants Fibroblasts Mice
spellingShingle	3T3-L1 fibroblasts Adipogenesis Hexavalent chromium PPAR gamma Proliferation chromium dexamethasone insulin isobutylmethylxanthine peroxisome proliferator activated receptor gamma triacylglycerol chromium chromium hexavalent ion pollutant adipocyte animal cell article cell count cell differentiation cell expansion cell proliferation cell strain 3T3 comparative study controlled study cytotoxicity test embryo immunofluorescence lipid storage micronucleus mitotic clonal expansion molecular dynamics mouse nonhuman Western blotting 3T3 cell line adipocyte animal cell differentiation cell proliferation cytology drug effects fibroblast physiology pollutant toxicity 3T3-L1 Cells Adipocytes Animals Cell Differentiation Cell Proliferation Chromium Environmental Pollutants Fibroblasts Mice Martini, C.N. Brandani, J.N. Gabrielli, M. Vila, M.D.C. Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
topic_facet	3T3-L1 fibroblasts Adipogenesis Hexavalent chromium PPAR gamma Proliferation chromium dexamethasone insulin isobutylmethylxanthine peroxisome proliferator activated receptor gamma triacylglycerol chromium chromium hexavalent ion pollutant adipocyte animal cell article cell count cell differentiation cell expansion cell proliferation cell strain 3T3 comparative study controlled study cytotoxicity test embryo immunofluorescence lipid storage micronucleus mitotic clonal expansion molecular dynamics mouse nonhuman Western blotting 3T3 cell line adipocyte animal cell differentiation cell proliferation cytology drug effects fibroblast physiology pollutant toxicity 3T3-L1 Cells Adipocytes Animals Cell Differentiation Cell Proliferation Chromium Environmental Pollutants Fibroblasts Mice
description	Heavy metals contamination has become an important risk factor for public health and the environment. Chromium is a frequent industrial contaminant and is also used in orthopaedic joint replacements made from cobalt-chromium-alloy. Since hexavalent chromium (Cr(VI)) was reported as genotoxic and carcinogenic in different mammals, to further evaluate its cytotoxicity, we investigated the effect of this heavy metal in the proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. These cells, after the addition of a mixture containing insulin, dexamethasone and methylisobutylxanthine, first proliferate, a process known as mitotic clonal expansion (MCE), and then differentiate to adipocytes. In this differentiation process a key transcription factor is induced: peroxisome proliferator-activated receptor gamma (PPAR gamma). We found that treatment of 3T3-L1 fibroblasts with potassium chromate inhibited proliferation in exponentially growing cells and MCE as well as differentiation. A decrease in PPAR gamma content, evaluated by western blot and immunofluorescence, was found in cells differentiated in the presence of chromium. On the other hand, after inhibition of differentiation with chromium, when the metal was removed, differentiation was recovered, which indicates that this may be a reversible effect. We also found an increase in the number of micronucleated cells after treatment with Cr(VI) which is associated with genotoxic effects. According to our results, Cr(VI) is able to inhibit proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts and to increase micronucleated cells, which are all indicative of alterations in cellular physiology and therefore, contributes to further elucidate the cytotoxic effects of this heavy metal. © 2014 Elsevier Ltd.
format	JOUR
author	Martini, C.N. Brandani, J.N. Gabrielli, M. Vila, M.D.C.
author_facet	Martini, C.N. Brandani, J.N. Gabrielli, M. Vila, M.D.C.
author_sort	Martini, C.N.
title	Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
title_short	Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
title_full	Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
title_fullStr	Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
title_full_unstemmed	Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
title_sort	effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3t3-l1 fibroblasts
url	http://hdl.handle.net/20.500.12110/paper_08872333_v28_n4_p700_Martini
work_keys_str_mv	AT martinicn effectofhexavalentchromiumonproliferationanddifferentiationtoadipocytesof3t3l1fibroblasts AT brandanijn effectofhexavalentchromiumonproliferationanddifferentiationtoadipocytesof3t3l1fibroblasts AT gabriellim effectofhexavalentchromiumonproliferationanddifferentiationtoadipocytesof3t3l1fibroblasts AT vilamdc effectofhexavalentchromiumonproliferationanddifferentiationtoadipocytesof3t3l1fibroblasts
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Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts

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