Cytogenetic evaluation of two nitroimidazole derivatives
5-Nitroimidazoles are a well-established group of antiprotozoal and antibacterial agents. Thanks to their antimicrobial activity these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa such as Trichomonas vaginalis, Entamoeba histolytica and Giardia...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08872333_v17_n1_p35_LopezNigro |
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todo:paper_08872333_v17_n1_p35_LopezNigro2023-10-03T15:40:48Z Cytogenetic evaluation of two nitroimidazole derivatives López Nigro, M.M. Palermo, A.M. Mudry, M.D. Carballo, M.A. Biomarkers Cytogenetics Genetic toxicology Metronidazole Ornidazole metronidazole nitroimidazole derivative ornidazol richet ornidazole adult analysis of variance article cell culture cell division chemical analysis chromosome aberration chromosome analysis clinical trial controlled clinical trial controlled study cytogenetics female genotoxicity human human cell lymphocyte male mitosis normal human risk assessment sister chromatid exchange Adult Antitrichomonal Agents Cell Culture Techniques Cell Division Chromosome Aberrations DNA Damage Dose-Response Relationship, Drug Female Humans Lymphocytes Male Metronidazole Mitosis Mutagenicity Tests Ornidazole Risk Assessment Sister Chromatid Exchange Entamoeba histolytica Giardia intestinalis Protozoa Trichomonas vaginalis 5-Nitroimidazoles are a well-established group of antiprotozoal and antibacterial agents. Thanks to their antimicrobial activity these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa such as Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. The aim of the present study is to achieve a precise characterization of the genotoxic activity of these compounds and to establish the value of cytogenetic assays in order to determine the effect of these drugs, at therapeutic doses, to settle an improved risk assessment. Two nitroimidazole were studied, metronidazole and ornidazole, at four different concentrations (0.1, 1, 10 and 50 μg/ml of peripheral blood lymphocyte culture). Endpoints analyzed included: mitotic index (MI), replication index (RI), sister chromatid exchange (SCE) and chromosomal aberrations (CA). An analysis of variance test (ANOVA) was performed to evaluate the results. A significant decrease (P<0.0001) in MI as well as an increase in SCE (P<0.0001) and CA (0.0001) frequencies for both drugs was observed. No modifications in RI were found. The results suggest a genotoxic and cytotoxic effect of MTZ and ONZ in human peripheral blood cultures in vitro. © 2002 Elsevier Science Ltd. All rights reserved. Fil:Palermo, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08872333_v17_n1_p35_LopezNigro |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomarkers Cytogenetics Genetic toxicology Metronidazole Ornidazole metronidazole nitroimidazole derivative ornidazol richet ornidazole adult analysis of variance article cell culture cell division chemical analysis chromosome aberration chromosome analysis clinical trial controlled clinical trial controlled study cytogenetics female genotoxicity human human cell lymphocyte male mitosis normal human risk assessment sister chromatid exchange Adult Antitrichomonal Agents Cell Culture Techniques Cell Division Chromosome Aberrations DNA Damage Dose-Response Relationship, Drug Female Humans Lymphocytes Male Metronidazole Mitosis Mutagenicity Tests Ornidazole Risk Assessment Sister Chromatid Exchange Entamoeba histolytica Giardia intestinalis Protozoa Trichomonas vaginalis |
| spellingShingle |
Biomarkers Cytogenetics Genetic toxicology Metronidazole Ornidazole metronidazole nitroimidazole derivative ornidazol richet ornidazole adult analysis of variance article cell culture cell division chemical analysis chromosome aberration chromosome analysis clinical trial controlled clinical trial controlled study cytogenetics female genotoxicity human human cell lymphocyte male mitosis normal human risk assessment sister chromatid exchange Adult Antitrichomonal Agents Cell Culture Techniques Cell Division Chromosome Aberrations DNA Damage Dose-Response Relationship, Drug Female Humans Lymphocytes Male Metronidazole Mitosis Mutagenicity Tests Ornidazole Risk Assessment Sister Chromatid Exchange Entamoeba histolytica Giardia intestinalis Protozoa Trichomonas vaginalis López Nigro, M.M. Palermo, A.M. Mudry, M.D. Carballo, M.A. Cytogenetic evaluation of two nitroimidazole derivatives |
| topic_facet |
Biomarkers Cytogenetics Genetic toxicology Metronidazole Ornidazole metronidazole nitroimidazole derivative ornidazol richet ornidazole adult analysis of variance article cell culture cell division chemical analysis chromosome aberration chromosome analysis clinical trial controlled clinical trial controlled study cytogenetics female genotoxicity human human cell lymphocyte male mitosis normal human risk assessment sister chromatid exchange Adult Antitrichomonal Agents Cell Culture Techniques Cell Division Chromosome Aberrations DNA Damage Dose-Response Relationship, Drug Female Humans Lymphocytes Male Metronidazole Mitosis Mutagenicity Tests Ornidazole Risk Assessment Sister Chromatid Exchange Entamoeba histolytica Giardia intestinalis Protozoa Trichomonas vaginalis |
| description |
5-Nitroimidazoles are a well-established group of antiprotozoal and antibacterial agents. Thanks to their antimicrobial activity these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa such as Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. The aim of the present study is to achieve a precise characterization of the genotoxic activity of these compounds and to establish the value of cytogenetic assays in order to determine the effect of these drugs, at therapeutic doses, to settle an improved risk assessment. Two nitroimidazole were studied, metronidazole and ornidazole, at four different concentrations (0.1, 1, 10 and 50 μg/ml of peripheral blood lymphocyte culture). Endpoints analyzed included: mitotic index (MI), replication index (RI), sister chromatid exchange (SCE) and chromosomal aberrations (CA). An analysis of variance test (ANOVA) was performed to evaluate the results. A significant decrease (P<0.0001) in MI as well as an increase in SCE (P<0.0001) and CA (0.0001) frequencies for both drugs was observed. No modifications in RI were found. The results suggest a genotoxic and cytotoxic effect of MTZ and ONZ in human peripheral blood cultures in vitro. © 2002 Elsevier Science Ltd. All rights reserved. |
| format |
JOUR |
| author |
López Nigro, M.M. Palermo, A.M. Mudry, M.D. Carballo, M.A. |
| author_facet |
López Nigro, M.M. Palermo, A.M. Mudry, M.D. Carballo, M.A. |
| author_sort |
López Nigro, M.M. |
| title |
Cytogenetic evaluation of two nitroimidazole derivatives |
| title_short |
Cytogenetic evaluation of two nitroimidazole derivatives |
| title_full |
Cytogenetic evaluation of two nitroimidazole derivatives |
| title_fullStr |
Cytogenetic evaluation of two nitroimidazole derivatives |
| title_full_unstemmed |
Cytogenetic evaluation of two nitroimidazole derivatives |
| title_sort |
cytogenetic evaluation of two nitroimidazole derivatives |
| url |
http://hdl.handle.net/20.500.12110/paper_08872333_v17_n1_p35_LopezNigro |
| work_keys_str_mv |
AT lopeznigromm cytogeneticevaluationoftwonitroimidazolederivatives AT palermoam cytogeneticevaluationoftwonitroimidazolederivatives AT mudrymd cytogeneticevaluationoftwonitroimidazolederivatives AT carballoma cytogeneticevaluationoftwonitroimidazolederivatives |
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1807318539720196096 |