Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes
Coagulase-negative staphylococci (CNS) are the most frequently isolated bacteria in cases of subclinical mastitis in dairy cows. CNS species may differ in their pathogenicity, but very little is known about their virulence factors or their immune response in intramammary infections. To our knowledge...
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todo:paper_08824010_v116_n_p146_Lasagno2023-10-03T15:40:22Z Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes Lasagno, M. Ortiz, M. Vissio, C. Yaciuk, R. Bonetto, C. Pellegrino, M. Bogni, C. Odierno, L. Raspanti, C. Bacterial growth Goat mastitis model Histopathology Somatic cell count Staphylococcus chromogenes animal experiment animal tissue Article bacterial shedding bacterial strain bovine mastitis controlled study inoculation lactation limit of quantitation milk nonhuman pathogenesis priority journal Saanen goat somatic cell Staphylococcus Staphylococcus chromogenes Coagulase-negative staphylococci (CNS) are the most frequently isolated bacteria in cases of subclinical mastitis in dairy cows. CNS species may differ in their pathogenicity, but very little is known about their virulence factors or their immune response in intramammary infections. To our knowledge, no experimental studies into the mastitis pathogenesis caused by CNS have been described in lactating goats. The aim of this study was to induce an experimentally Staphylococcus chromogenes mastitis in lactating goats aimed at verifying if the model can be used to evaluate the inflammatory response, the dynamics of infection and the pathological findings within the first hours of intramammary inoculation. Six Saanen goats in mid-lactation were inoculated with 1 × 107 colony forming units of S. chromogenes. Bacterial growth peaked in milk from the challenged right halves of the mammary glands (RMG) at 4 h post inoculation (PI). Shedding of viable bacteria showed a marked decrease at 12 h PI. An increase in mean somatic cell counts was observed in the milk samples from 8 h PI onwards. Mild clinical signs were evoked by intramammary inoculation. Staphylococcus chromogenes could be isolated in tissue from all RMG. Histological examination of specimens of the RMG and lymph nodes of the goats showed an increased inflammatory response throughout the experiment with respect to control halves. In conclusion, the experimental inoculation of S. chromogenes in lactating goats is capable of eliciting an inflammatory response and capable of causing pathological changes. This research represents a preliminary study for a better knowledge of the mastitis pathogenesis caused by S. chromogenes. © 2018 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08824010_v116_n_p146_Lasagno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacterial growth Goat mastitis model Histopathology Somatic cell count Staphylococcus chromogenes animal experiment animal tissue Article bacterial shedding bacterial strain bovine mastitis controlled study inoculation lactation limit of quantitation milk nonhuman pathogenesis priority journal Saanen goat somatic cell Staphylococcus Staphylococcus chromogenes |
| spellingShingle |
Bacterial growth Goat mastitis model Histopathology Somatic cell count Staphylococcus chromogenes animal experiment animal tissue Article bacterial shedding bacterial strain bovine mastitis controlled study inoculation lactation limit of quantitation milk nonhuman pathogenesis priority journal Saanen goat somatic cell Staphylococcus Staphylococcus chromogenes Lasagno, M. Ortiz, M. Vissio, C. Yaciuk, R. Bonetto, C. Pellegrino, M. Bogni, C. Odierno, L. Raspanti, C. Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| topic_facet |
Bacterial growth Goat mastitis model Histopathology Somatic cell count Staphylococcus chromogenes animal experiment animal tissue Article bacterial shedding bacterial strain bovine mastitis controlled study inoculation lactation limit of quantitation milk nonhuman pathogenesis priority journal Saanen goat somatic cell Staphylococcus Staphylococcus chromogenes |
| description |
Coagulase-negative staphylococci (CNS) are the most frequently isolated bacteria in cases of subclinical mastitis in dairy cows. CNS species may differ in their pathogenicity, but very little is known about their virulence factors or their immune response in intramammary infections. To our knowledge, no experimental studies into the mastitis pathogenesis caused by CNS have been described in lactating goats. The aim of this study was to induce an experimentally Staphylococcus chromogenes mastitis in lactating goats aimed at verifying if the model can be used to evaluate the inflammatory response, the dynamics of infection and the pathological findings within the first hours of intramammary inoculation. Six Saanen goats in mid-lactation were inoculated with 1 × 107 colony forming units of S. chromogenes. Bacterial growth peaked in milk from the challenged right halves of the mammary glands (RMG) at 4 h post inoculation (PI). Shedding of viable bacteria showed a marked decrease at 12 h PI. An increase in mean somatic cell counts was observed in the milk samples from 8 h PI onwards. Mild clinical signs were evoked by intramammary inoculation. Staphylococcus chromogenes could be isolated in tissue from all RMG. Histological examination of specimens of the RMG and lymph nodes of the goats showed an increased inflammatory response throughout the experiment with respect to control halves. In conclusion, the experimental inoculation of S. chromogenes in lactating goats is capable of eliciting an inflammatory response and capable of causing pathological changes. This research represents a preliminary study for a better knowledge of the mastitis pathogenesis caused by S. chromogenes. © 2018 Elsevier Ltd |
| format |
JOUR |
| author |
Lasagno, M. Ortiz, M. Vissio, C. Yaciuk, R. Bonetto, C. Pellegrino, M. Bogni, C. Odierno, L. Raspanti, C. |
| author_facet |
Lasagno, M. Ortiz, M. Vissio, C. Yaciuk, R. Bonetto, C. Pellegrino, M. Bogni, C. Odierno, L. Raspanti, C. |
| author_sort |
Lasagno, M. |
| title |
Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| title_short |
Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| title_full |
Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| title_fullStr |
Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| title_full_unstemmed |
Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes |
| title_sort |
pathogenesis and inflammatory response in experimental caprine mastitis due to staphylococcus chromogenes |
| url |
http://hdl.handle.net/20.500.12110/paper_08824010_v116_n_p146_Lasagno |
| work_keys_str_mv |
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| _version_ |
1782030478341046272 |