Ribosomal DNA variation in the grasshopper, Dichroplus elongatus
We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that s...
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08312796_v45_n6_p1125_Clemente |
| Aporte de: |
| id |
todo:paper_08312796_v45_n6_p1125_Clemente |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_08312796_v45_n6_p1125_Clemente2023-10-03T15:40:14Z Ribosomal DNA variation in the grasshopper, Dichroplus elongatus Clemente, M. Remis, M.I. Vilardi, J.C. Dichroplus elongatus Environmental variables RFLP Ribosomal DNA Dichroplus elongatus ribosome DNA animal article genetics grasshopper restriction fragment length polymorphism Southern blotting Animals Blotting, Southern DNA, Ribosomal Grasshoppers Polymorphism, Restriction Fragment Length We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that showed no variation. The remaining enzymes showed fragment size variation at both intra- and interpopulation levels. Stepwise regression analysis revealed that the average number of length variants per individual is significantly associated with altitude. Moreover, the same analysis indicated that the frequency of some restriction variants exhibits a significant regression on both geographic and climatic variables. The intra- and interpopulation variability of rDNA was analysed by Lynch's and Hedrick's similarity indices using presence or absence of a fragment and band intensities, respectively. The corresponding neighbour-joining (N-J) trees based on Lynch's and Hedrick's genetic distances resulted in similar topologies. However, these trees were not in agreement with the N-J dendrogram obtained from mtDNA data previously reported by Clemente et al. (2000). The disagreement between mtDNA and rDNA phenograms along with the observed correlation between rDNA variability and geographical and climatic variables suggest some form of selection, besides genetic drift and migration, is involved in the pattern of rDNA variation. © 2002 NRC. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08312796_v45_n6_p1125_Clemente |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dichroplus elongatus Environmental variables RFLP Ribosomal DNA Dichroplus elongatus ribosome DNA animal article genetics grasshopper restriction fragment length polymorphism Southern blotting Animals Blotting, Southern DNA, Ribosomal Grasshoppers Polymorphism, Restriction Fragment Length |
| spellingShingle |
Dichroplus elongatus Environmental variables RFLP Ribosomal DNA Dichroplus elongatus ribosome DNA animal article genetics grasshopper restriction fragment length polymorphism Southern blotting Animals Blotting, Southern DNA, Ribosomal Grasshoppers Polymorphism, Restriction Fragment Length Clemente, M. Remis, M.I. Vilardi, J.C. Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| topic_facet |
Dichroplus elongatus Environmental variables RFLP Ribosomal DNA Dichroplus elongatus ribosome DNA animal article genetics grasshopper restriction fragment length polymorphism Southern blotting Animals Blotting, Southern DNA, Ribosomal Grasshoppers Polymorphism, Restriction Fragment Length |
| description |
We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that showed no variation. The remaining enzymes showed fragment size variation at both intra- and interpopulation levels. Stepwise regression analysis revealed that the average number of length variants per individual is significantly associated with altitude. Moreover, the same analysis indicated that the frequency of some restriction variants exhibits a significant regression on both geographic and climatic variables. The intra- and interpopulation variability of rDNA was analysed by Lynch's and Hedrick's similarity indices using presence or absence of a fragment and band intensities, respectively. The corresponding neighbour-joining (N-J) trees based on Lynch's and Hedrick's genetic distances resulted in similar topologies. However, these trees were not in agreement with the N-J dendrogram obtained from mtDNA data previously reported by Clemente et al. (2000). The disagreement between mtDNA and rDNA phenograms along with the observed correlation between rDNA variability and geographical and climatic variables suggest some form of selection, besides genetic drift and migration, is involved in the pattern of rDNA variation. © 2002 NRC. |
| format |
JOUR |
| author |
Clemente, M. Remis, M.I. Vilardi, J.C. |
| author_facet |
Clemente, M. Remis, M.I. Vilardi, J.C. |
| author_sort |
Clemente, M. |
| title |
Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| title_short |
Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| title_full |
Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| title_fullStr |
Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| title_full_unstemmed |
Ribosomal DNA variation in the grasshopper, Dichroplus elongatus |
| title_sort |
ribosomal dna variation in the grasshopper, dichroplus elongatus |
| url |
http://hdl.handle.net/20.500.12110/paper_08312796_v45_n6_p1125_Clemente |
| work_keys_str_mv |
AT clementem ribosomaldnavariationinthegrasshopperdichropluselongatus AT remismi ribosomaldnavariationinthegrasshopperdichropluselongatus AT vilardijc ribosomaldnavariationinthegrasshopperdichropluselongatus |
| _version_ |
1807319523540336640 |